Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica
Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms an...
Published in: | Electronic Journal of Biotechnology |
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UNIV CATOLICA DE VALPARAISO
2020
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Online Access: | http://nora.nerc.ac.uk/id/eprint/523480/ https://nora.nerc.ac.uk/id/eprint/523480/1/1-s2.0-S0717345820300361-main.pdf https://www.sciencedirect.com/science/article/pii/S0717345820300361 |
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ftnerc:oai:nora.nerc.ac.uk:523480 2023-05-15T13:41:43+02:00 Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica Ibrahim, Salihu Zahri, Khadijah Nabilah Mohd Convey, Peter Khalil, Khalilah Abdul Gomez Fuentes, Claudio Zulkarnain, Azham Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima 2020-11-01 text http://nora.nerc.ac.uk/id/eprint/523480/ https://nora.nerc.ac.uk/id/eprint/523480/1/1-s2.0-S0717345820300361-main.pdf https://www.sciencedirect.com/science/article/pii/S0717345820300361 en eng UNIV CATOLICA DE VALPARAISO https://nora.nerc.ac.uk/id/eprint/523480/1/1-s2.0-S0717345820300361-main.pdf Ibrahim, Salihu; Zahri, Khadijah Nabilah Mohd; Convey, Peter orcid:0000-0001-8497-9903 Khalil, Khalilah Abdul; Gomez Fuentes, Claudio; Zulkarnain, Azham; Alias, Siti Aisyah; González-Rocha, Gerardo; Ahmad, Siti Aqlima. 2020 Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica. Electronic Journal of Biotechnology, 48. 1-12. https://doi.org/10.1016/j.ejbt.2020.07.005 <https://doi.org/10.1016/j.ejbt.2020.07.005> cc_by_nc_nd_4 CC-BY-NC-ND Publication - Article PeerReviewed 2020 ftnerc https://doi.org/10.1016/j.ejbt.2020.07.005 2023-02-04T19:48:25Z Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. Results: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. Conclusions: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctica Natural Environment Research Council: NERC Open Research Archive Antarctic Electronic Journal of Biotechnology 48 1 12 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
English |
description |
Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. Results: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. Conclusions: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica. |
format |
Article in Journal/Newspaper |
author |
Ibrahim, Salihu Zahri, Khadijah Nabilah Mohd Convey, Peter Khalil, Khalilah Abdul Gomez Fuentes, Claudio Zulkarnain, Azham Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
spellingShingle |
Ibrahim, Salihu Zahri, Khadijah Nabilah Mohd Convey, Peter Khalil, Khalilah Abdul Gomez Fuentes, Claudio Zulkarnain, Azham Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
author_facet |
Ibrahim, Salihu Zahri, Khadijah Nabilah Mohd Convey, Peter Khalil, Khalilah Abdul Gomez Fuentes, Claudio Zulkarnain, Azham Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
author_sort |
Ibrahim, Salihu |
title |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_short |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_full |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_fullStr |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_full_unstemmed |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_sort |
optimisation of biodegradation conditions for waste canola oil by cold-adapted rhodococcus sp. aq5-07 from antarctica |
publisher |
UNIV CATOLICA DE VALPARAISO |
publishDate |
2020 |
url |
http://nora.nerc.ac.uk/id/eprint/523480/ https://nora.nerc.ac.uk/id/eprint/523480/1/1-s2.0-S0717345820300361-main.pdf https://www.sciencedirect.com/science/article/pii/S0717345820300361 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
https://nora.nerc.ac.uk/id/eprint/523480/1/1-s2.0-S0717345820300361-main.pdf Ibrahim, Salihu; Zahri, Khadijah Nabilah Mohd; Convey, Peter orcid:0000-0001-8497-9903 Khalil, Khalilah Abdul; Gomez Fuentes, Claudio; Zulkarnain, Azham; Alias, Siti Aisyah; González-Rocha, Gerardo; Ahmad, Siti Aqlima. 2020 Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica. Electronic Journal of Biotechnology, 48. 1-12. https://doi.org/10.1016/j.ejbt.2020.07.005 <https://doi.org/10.1016/j.ejbt.2020.07.005> |
op_rights |
cc_by_nc_nd_4 |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.ejbt.2020.07.005 |
container_title |
Electronic Journal of Biotechnology |
container_volume |
48 |
container_start_page |
1 |
op_container_end_page |
12 |
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1766155026828361728 |