Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake
Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repeatability, which is critical for understanding the...
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ftnerc:oai:nora.nerc.ac.uk:522956 2023-05-15T14:30:11+02:00 Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake Handley, Lori Lawson Read, Daniel S. Winfield, Ian J. Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd 2019-05 text http://nora.nerc.ac.uk/id/eprint/522956/ https://nora.nerc.ac.uk/id/eprint/522956/1/N522956JA.pdf https://doi.org/10.1002/edn3.5 en eng Wiley https://nora.nerc.ac.uk/id/eprint/522956/1/N522956JA.pdf Handley, Lori Lawson; Read, Daniel S.; Winfield, Ian J.; Kimbell, Helen; Johnson, Harriet; Li, Jianlong; Hahn, Christoph; Blackman, Rosetta; Wilcox, Rose; Donnelly, Rob; Szitenberg, Amir; Hänfling, Bernd. 2019 Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake. Environmental DNA, 1 (1). 26-39. https://doi.org/10.1002/edn3.5 <https://doi.org/10.1002/edn3.5> cc_by_4 CC-BY Ecology and Environment Publication - Article PeerReviewed 2019 ftnerc https://doi.org/10.1002/edn3.5 2023-02-04T19:48:08Z Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repeatability, which is critical for understanding the ecology of eDNA and developing effective sampling strategies. Here, we carried out comprehensive spatial sampling of England's largest lake, Windermere, during summer and winter to (1) examine repeatability of the method, (2) compare eDNA results with contemporary gill‐net survey data, (3) test the hypothesis of greater spatial structure of eDNA in summer compared to winter due to differences in water mixing between seasons, and (4) compare the effectiveness of shore and offshore sampling for species detection. We find broad consistency between the results from three sampling events in terms of species detection and abundance, with eDNA detecting more species than established methods and being significantly correlated with rank abundance determined by long‐term data. As predicted, spatial structure was much greater in the summer, reflecting less mixing of eDNA than in the winter. For example Arctic charr, a deepwater species, was only detected in deep, midlake samples in the summer, while littoral or benthic species such as minnow and stickleback were more frequently detected in shore samples. By contrast in winter, the eDNA of these species was more uniformly distributed. This has important implications for design of sampling campaigns, for example, deep‐water species could be missed and littoral/benthic species overrepresented by focusing exclusively on shoreline samples collected in the summer. Article in Journal/Newspaper Arctic charr Arctic Natural Environment Research Council: NERC Open Research Archive Arctic Environmental DNA 1 1 26 39 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
topic |
Ecology and Environment |
spellingShingle |
Ecology and Environment Handley, Lori Lawson Read, Daniel S. Winfield, Ian J. Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
topic_facet |
Ecology and Environment |
description |
Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repeatability, which is critical for understanding the ecology of eDNA and developing effective sampling strategies. Here, we carried out comprehensive spatial sampling of England's largest lake, Windermere, during summer and winter to (1) examine repeatability of the method, (2) compare eDNA results with contemporary gill‐net survey data, (3) test the hypothesis of greater spatial structure of eDNA in summer compared to winter due to differences in water mixing between seasons, and (4) compare the effectiveness of shore and offshore sampling for species detection. We find broad consistency between the results from three sampling events in terms of species detection and abundance, with eDNA detecting more species than established methods and being significantly correlated with rank abundance determined by long‐term data. As predicted, spatial structure was much greater in the summer, reflecting less mixing of eDNA than in the winter. For example Arctic charr, a deepwater species, was only detected in deep, midlake samples in the summer, while littoral or benthic species such as minnow and stickleback were more frequently detected in shore samples. By contrast in winter, the eDNA of these species was more uniformly distributed. This has important implications for design of sampling campaigns, for example, deep‐water species could be missed and littoral/benthic species overrepresented by focusing exclusively on shoreline samples collected in the summer. |
format |
Article in Journal/Newspaper |
author |
Handley, Lori Lawson Read, Daniel S. Winfield, Ian J. Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd |
author_facet |
Handley, Lori Lawson Read, Daniel S. Winfield, Ian J. Kimbell, Helen Johnson, Harriet Li, Jianlong Hahn, Christoph Blackman, Rosetta Wilcox, Rose Donnelly, Rob Szitenberg, Amir Hänfling, Bernd |
author_sort |
Handley, Lori Lawson |
title |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_short |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_full |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_fullStr |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_full_unstemmed |
Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake |
title_sort |
temporal and spatial variation in distribution of fish environmental dna in england’s largest lake |
publisher |
Wiley |
publishDate |
2019 |
url |
http://nora.nerc.ac.uk/id/eprint/522956/ https://nora.nerc.ac.uk/id/eprint/522956/1/N522956JA.pdf https://doi.org/10.1002/edn3.5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic |
genre_facet |
Arctic charr Arctic |
op_relation |
https://nora.nerc.ac.uk/id/eprint/522956/1/N522956JA.pdf Handley, Lori Lawson; Read, Daniel S.; Winfield, Ian J.; Kimbell, Helen; Johnson, Harriet; Li, Jianlong; Hahn, Christoph; Blackman, Rosetta; Wilcox, Rose; Donnelly, Rob; Szitenberg, Amir; Hänfling, Bernd. 2019 Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake. Environmental DNA, 1 (1). 26-39. https://doi.org/10.1002/edn3.5 <https://doi.org/10.1002/edn3.5> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/edn3.5 |
container_title |
Environmental DNA |
container_volume |
1 |
container_issue |
1 |
container_start_page |
26 |
op_container_end_page |
39 |
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1766304083949387776 |