Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica
In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarcti...
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ftnerc:oai:nora.nerc.ac.uk:521659 2023-05-15T13:41:42+02:00 Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica Salwoom, Leelatulasi Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Shariff, Fairolniza Mohd Convey, Peter Pearce, David Mohamad Ali, Mohd Shukuri 2019-02-16 text http://nora.nerc.ac.uk/id/eprint/521659/ https://nora.nerc.ac.uk/id/eprint/521659/1/molecules-24-00715.pdf https://www.mdpi.com/1420-3049/24/4/715 en eng https://nora.nerc.ac.uk/id/eprint/521659/1/molecules-24-00715.pdf Salwoom, Leelatulasi; Raja Abd Rahman, Raja Noor Zaliha; Salleh, Abu Bakar; Shariff, Fairolniza Mohd; Convey, Peter orcid:0000-0001-8497-9903 Pearce, David orcid:0000-0001-5292-4596 Mohamad Ali, Mohd Shukuri. 2019 Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica. Molecules, 24 (4), 715. https://doi.org/10.3390/molecules24040715 <https://doi.org/10.3390/molecules24040715> cc_by_4 CC-BY Publication - Article PeerReviewed 2019 ftnerc https://doi.org/10.3390/molecules24040715 2023-02-04T19:47:27Z In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation. Article in Journal/Newspaper Antarc* Antarctic Antarctica Signy Island South Orkney Islands Natural Environment Research Council: NERC Open Research Archive Antarctic South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) Signy Station ENVELOPE(-45.600,-45.600,-60.717,-60.717) Molecules 24 4 715 |
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Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation. |
format |
Article in Journal/Newspaper |
author |
Salwoom, Leelatulasi Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Shariff, Fairolniza Mohd Convey, Peter Pearce, David Mohamad Ali, Mohd Shukuri |
spellingShingle |
Salwoom, Leelatulasi Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Shariff, Fairolniza Mohd Convey, Peter Pearce, David Mohamad Ali, Mohd Shukuri Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica |
author_facet |
Salwoom, Leelatulasi Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Shariff, Fairolniza Mohd Convey, Peter Pearce, David Mohamad Ali, Mohd Shukuri |
author_sort |
Salwoom, Leelatulasi |
title |
Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica |
title_short |
Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica |
title_full |
Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica |
title_fullStr |
Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica |
title_full_unstemmed |
Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica |
title_sort |
isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from signy island, antarctica |
publishDate |
2019 |
url |
http://nora.nerc.ac.uk/id/eprint/521659/ https://nora.nerc.ac.uk/id/eprint/521659/1/molecules-24-00715.pdf https://www.mdpi.com/1420-3049/24/4/715 |
long_lat |
ENVELOPE(-45.500,-45.500,-60.583,-60.583) ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(-45.600,-45.600,-60.717,-60.717) |
geographic |
Antarctic South Orkney Islands Signy Island Signy Station |
geographic_facet |
Antarctic South Orkney Islands Signy Island Signy Station |
genre |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
op_relation |
https://nora.nerc.ac.uk/id/eprint/521659/1/molecules-24-00715.pdf Salwoom, Leelatulasi; Raja Abd Rahman, Raja Noor Zaliha; Salleh, Abu Bakar; Shariff, Fairolniza Mohd; Convey, Peter orcid:0000-0001-8497-9903 Pearce, David orcid:0000-0001-5292-4596 Mohamad Ali, Mohd Shukuri. 2019 Isolation, characterisation and lipase production of a cold-adapted bacterial strain isolated from Signy Island, Antarctica. Molecules, 24 (4), 715. https://doi.org/10.3390/molecules24040715 <https://doi.org/10.3390/molecules24040715> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/molecules24040715 |
container_title |
Molecules |
container_volume |
24 |
container_issue |
4 |
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715 |
_version_ |
1766154155046469632 |