Lactate dehydrogenases in antarctic and temperate fish species
1. The kinetics of lactate dehydrogenase (both forward and back reaction) in cardiac and skeletal muscle of an Antarctic teleost have been compared with a temperate teleost of comparable morphology and ecology. 2. In both species the forward reaction (pyruvate to lactate) is maximally activated at 2...
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1988
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ftnerc:oai:nora.nerc.ac.uk:521624 2023-05-15T13:41:42+02:00 Lactate dehydrogenases in antarctic and temperate fish species Fitch, N.A. 1988 http://nora.nerc.ac.uk/id/eprint/521624/ https://doi.org/10.1016/0305-0491(88)90190-3 unknown Elsevier Fitch, N.A. 1988 Lactate dehydrogenases in antarctic and temperate fish species. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 91 (4). 671-676. https://doi.org/10.1016/0305-0491(88)90190-3 <https://doi.org/10.1016/0305-0491(88)90190-3> Publication - Article PeerReviewed 1988 ftnerc https://doi.org/10.1016/0305-0491(88)90190-3 2023-02-04T19:47:27Z 1. The kinetics of lactate dehydrogenase (both forward and back reaction) in cardiac and skeletal muscle of an Antarctic teleost have been compared with a temperate teleost of comparable morphology and ecology. 2. In both species the forward reaction (pyruvate to lactate) is maximally activated at 2.5–4 mM pyruvate and inhibited above this level. 3. The Michaelis constant (Km) for pyruvate is not significantly different between muscle types or between species when measured at their normal environmental temperature. 4. Km for pyruvate varies with temperature in a positive direction. 5. The back reaction (lactate to pyruvate) is maximally activated by 12–16 mM lactate but only in skeletal muscle of the antarctic species is there inhibition above this level. 6. 6. The Km for lactate is significantly (P<0.05) lower in the Antarctic fish cardiac muscle. 7. 7. While the two species are morphologically and ecologically similar, differences at the biochemical level are discussed with respect to environmental temperature range and conservation of enzymic characteristics. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic The Antarctic Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 91 4 671 676 |
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Natural Environment Research Council: NERC Open Research Archive |
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1. The kinetics of lactate dehydrogenase (both forward and back reaction) in cardiac and skeletal muscle of an Antarctic teleost have been compared with a temperate teleost of comparable morphology and ecology. 2. In both species the forward reaction (pyruvate to lactate) is maximally activated at 2.5–4 mM pyruvate and inhibited above this level. 3. The Michaelis constant (Km) for pyruvate is not significantly different between muscle types or between species when measured at their normal environmental temperature. 4. Km for pyruvate varies with temperature in a positive direction. 5. The back reaction (lactate to pyruvate) is maximally activated by 12–16 mM lactate but only in skeletal muscle of the antarctic species is there inhibition above this level. 6. 6. The Km for lactate is significantly (P<0.05) lower in the Antarctic fish cardiac muscle. 7. 7. While the two species are morphologically and ecologically similar, differences at the biochemical level are discussed with respect to environmental temperature range and conservation of enzymic characteristics. |
format |
Article in Journal/Newspaper |
author |
Fitch, N.A. |
spellingShingle |
Fitch, N.A. Lactate dehydrogenases in antarctic and temperate fish species |
author_facet |
Fitch, N.A. |
author_sort |
Fitch, N.A. |
title |
Lactate dehydrogenases in antarctic and temperate fish species |
title_short |
Lactate dehydrogenases in antarctic and temperate fish species |
title_full |
Lactate dehydrogenases in antarctic and temperate fish species |
title_fullStr |
Lactate dehydrogenases in antarctic and temperate fish species |
title_full_unstemmed |
Lactate dehydrogenases in antarctic and temperate fish species |
title_sort |
lactate dehydrogenases in antarctic and temperate fish species |
publisher |
Elsevier |
publishDate |
1988 |
url |
http://nora.nerc.ac.uk/id/eprint/521624/ https://doi.org/10.1016/0305-0491(88)90190-3 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Fitch, N.A. 1988 Lactate dehydrogenases in antarctic and temperate fish species. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 91 (4). 671-676. https://doi.org/10.1016/0305-0491(88)90190-3 <https://doi.org/10.1016/0305-0491(88)90190-3> |
op_doi |
https://doi.org/10.1016/0305-0491(88)90190-3 |
container_title |
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry |
container_volume |
91 |
container_issue |
4 |
container_start_page |
671 |
op_container_end_page |
676 |
_version_ |
1766154123338579968 |