Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp.
Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antar...
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Online Access: | http://nora.nerc.ac.uk/id/eprint/520620/ https://nora.nerc.ac.uk/id/eprint/520620/1/catalysts-12-01422.pdf https://doi.org/10.3390/catal12111422 |
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ftnerc:oai:nora.nerc.ac.uk:520620 2023-05-15T13:49:35+02:00 Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Zhari, Khadijah NabilahMohd Ahmad, Siti Aqlima 2022-11-12 text http://nora.nerc.ac.uk/id/eprint/520620/ https://nora.nerc.ac.uk/id/eprint/520620/1/catalysts-12-01422.pdf https://doi.org/10.3390/catal12111422 en eng MDPI https://nora.nerc.ac.uk/id/eprint/520620/1/catalysts-12-01422.pdf Lee, Gillian Li Yin; Zakaria, Nur Nadhirah; Futamata, Hiroyuki; Suzuki, Kenshi; Zulkharnain, Azham; Shaharuddin, Noor Azmi; Convey, Peter orcid:0000-0001-8497-9903 Zhari, Khadijah NabilahMohd; Ahmad, Siti Aqlima. 2022 Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. Catalysts, 12, 1422. 16, pp. https://doi.org/10.3390/catal12111422 <https://doi.org/10.3390/catal12111422> cc_by_4 CC-BY Publication - Article PeerReviewed 2022 ftnerc https://doi.org/10.3390/catal12111422 2023-02-04T19:46:51Z Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding phenol degradative enzymes identified using whole genome sequencing (WGS) were investigated to determine the pathway(s) of phenol degradation of Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctica. Complete phenol degradative genes involved only in the ortho-cleavage were detected in both strains. This was validated using assays of the enzymes catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, which indicated the activity of only catechol 1,2-dioxygenase in both strains, in agreement with the results from the WGS. Both strains were psychrotolerant with the optimum temperature for phenol degradation, being between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol pollution in cold environments. Article in Journal/Newspaper Antarc* Antarctic Antarctica Natural Environment Research Council: NERC Open Research Archive Antarctic Catalysts 12 11 1422 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding phenol degradative enzymes identified using whole genome sequencing (WGS) were investigated to determine the pathway(s) of phenol degradation of Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctica. Complete phenol degradative genes involved only in the ortho-cleavage were detected in both strains. This was validated using assays of the enzymes catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, which indicated the activity of only catechol 1,2-dioxygenase in both strains, in agreement with the results from the WGS. Both strains were psychrotolerant with the optimum temperature for phenol degradation, being between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol pollution in cold environments. |
format |
Article in Journal/Newspaper |
author |
Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Zhari, Khadijah NabilahMohd Ahmad, Siti Aqlima |
spellingShingle |
Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Zhari, Khadijah NabilahMohd Ahmad, Siti Aqlima Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
author_facet |
Lee, Gillian Li Yin Zakaria, Nur Nadhirah Futamata, Hiroyuki Suzuki, Kenshi Zulkharnain, Azham Shaharuddin, Noor Azmi Convey, Peter Zhari, Khadijah NabilahMohd Ahmad, Siti Aqlima |
author_sort |
Lee, Gillian Li Yin |
title |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_short |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_full |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_fullStr |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_full_unstemmed |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_sort |
metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, arthrobacter sp. |
publisher |
MDPI |
publishDate |
2022 |
url |
http://nora.nerc.ac.uk/id/eprint/520620/ https://nora.nerc.ac.uk/id/eprint/520620/1/catalysts-12-01422.pdf https://doi.org/10.3390/catal12111422 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
https://nora.nerc.ac.uk/id/eprint/520620/1/catalysts-12-01422.pdf Lee, Gillian Li Yin; Zakaria, Nur Nadhirah; Futamata, Hiroyuki; Suzuki, Kenshi; Zulkharnain, Azham; Shaharuddin, Noor Azmi; Convey, Peter orcid:0000-0001-8497-9903 Zhari, Khadijah NabilahMohd; Ahmad, Siti Aqlima. 2022 Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. Catalysts, 12, 1422. 16, pp. https://doi.org/10.3390/catal12111422 <https://doi.org/10.3390/catal12111422> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/catal12111422 |
container_title |
Catalysts |
container_volume |
12 |
container_issue |
11 |
container_start_page |
1422 |
_version_ |
1766251798530621440 |