Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently eme...
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ftnerc:oai:nora.nerc.ac.uk:519781 2023-05-15T13:49:35+02:00 Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method Kleinteich, Julia Puddick, Jonathan Wood, Susanna Hildebrand, Falk Laughinghouse IV, H. Pearce, David Dietrich, Daniel Wilmotte, Annick 2018-04 text http://nora.nerc.ac.uk/id/eprint/519781/ https://nora.nerc.ac.uk/id/eprint/519781/1/toxins-10-00147-v2.pdf https://doi.org/10.3390/toxins10040147 en eng MDPI AG https://nora.nerc.ac.uk/id/eprint/519781/1/toxins-10-00147-v2.pdf Kleinteich, Julia; Puddick, Jonathan; Wood, Susanna; Hildebrand, Falk; Laughinghouse IV, H.; Pearce, David orcid:0000-0001-5292-4596 Dietrich, Daniel; Wilmotte, Annick. 2018 Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method. Toxins, 10 (4), 147. 15, pp. https://doi.org/10.3390/toxins10040147 <https://doi.org/10.3390/toxins10040147> cc_by_4 CC-BY Publication - Article PeerReviewed 2018 ftnerc https://doi.org/10.3390/toxins10040147 2023-02-04T19:46:24Z Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp3, ADMAdda5, Dhb7] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen Article in Journal/Newspaper Antarc* Antarctic Arctic Svalbard Natural Environment Research Council: NERC Open Research Archive Arctic Antarctic Svalbard Svalbard Archipelago Toxins 10 4 147 |
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Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp3, ADMAdda5, Dhb7] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen |
format |
Article in Journal/Newspaper |
author |
Kleinteich, Julia Puddick, Jonathan Wood, Susanna Hildebrand, Falk Laughinghouse IV, H. Pearce, David Dietrich, Daniel Wilmotte, Annick |
spellingShingle |
Kleinteich, Julia Puddick, Jonathan Wood, Susanna Hildebrand, Falk Laughinghouse IV, H. Pearce, David Dietrich, Daniel Wilmotte, Annick Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
author_facet |
Kleinteich, Julia Puddick, Jonathan Wood, Susanna Hildebrand, Falk Laughinghouse IV, H. Pearce, David Dietrich, Daniel Wilmotte, Annick |
author_sort |
Kleinteich, Julia |
title |
Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
title_short |
Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
title_full |
Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
title_fullStr |
Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
title_full_unstemmed |
Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
title_sort |
toxic cyanobacteria in svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
publisher |
MDPI AG |
publishDate |
2018 |
url |
http://nora.nerc.ac.uk/id/eprint/519781/ https://nora.nerc.ac.uk/id/eprint/519781/1/toxins-10-00147-v2.pdf https://doi.org/10.3390/toxins10040147 |
geographic |
Arctic Antarctic Svalbard Svalbard Archipelago |
geographic_facet |
Arctic Antarctic Svalbard Svalbard Archipelago |
genre |
Antarc* Antarctic Arctic Svalbard |
genre_facet |
Antarc* Antarctic Arctic Svalbard |
op_relation |
https://nora.nerc.ac.uk/id/eprint/519781/1/toxins-10-00147-v2.pdf Kleinteich, Julia; Puddick, Jonathan; Wood, Susanna; Hildebrand, Falk; Laughinghouse IV, H.; Pearce, David orcid:0000-0001-5292-4596 Dietrich, Daniel; Wilmotte, Annick. 2018 Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method. Toxins, 10 (4), 147. 15, pp. https://doi.org/10.3390/toxins10040147 <https://doi.org/10.3390/toxins10040147> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/toxins10040147 |
container_title |
Toxins |
container_volume |
10 |
container_issue |
4 |
container_start_page |
147 |
_version_ |
1766251759925198848 |