Characterization and temperature dependence of Arctic Micromonas polaris viruses
Global climate change-induced warming of the Artic seas is predicted to shift the phytoplankton community towards dominance of smaller-sized species due to global warming. Yet, little is known about their viral mortality agents despite the ecological importance of viruses regulating phytoplankton ho...
Published in: | Viruses |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2017
|
Subjects: | |
Online Access: | http://nora.nerc.ac.uk/id/eprint/517558/ https://nora.nerc.ac.uk/id/eprint/517558/1/viruses-09-00134-v4.pdf https://doi.org/10.3390/v9060134 |
id |
ftnerc:oai:nora.nerc.ac.uk:517558 |
---|---|
record_format |
openpolar |
spelling |
ftnerc:oai:nora.nerc.ac.uk:517558 2023-05-15T14:26:44+02:00 Characterization and temperature dependence of Arctic Micromonas polaris viruses Maat, Douwe S. Biggs, Tristan Evans, Claire van Bleijswijk, Judith D.L. van der Wel, Nicole N. Dutilh, Bas E. Brussaard, Corina P.D. 2017-06 text http://nora.nerc.ac.uk/id/eprint/517558/ https://nora.nerc.ac.uk/id/eprint/517558/1/viruses-09-00134-v4.pdf https://doi.org/10.3390/v9060134 en eng https://nora.nerc.ac.uk/id/eprint/517558/1/viruses-09-00134-v4.pdf Maat, Douwe S.; Biggs, Tristan; Evans, Claire orcid:0000-0003-0569-7057 van Bleijswijk, Judith D.L.; van der Wel, Nicole N.; Dutilh, Bas E.; Brussaard, Corina P.D. 2017 Characterization and temperature dependence of Arctic Micromonas polaris viruses. Viruses, 9 (6). 134. https://doi.org/10.3390/v9060134 <https://doi.org/10.3390/v9060134> cc_by_4 CC-BY Publication - Article PeerReviewed 2017 ftnerc https://doi.org/10.3390/v9060134 2023-02-04T19:45:13Z Global climate change-induced warming of the Artic seas is predicted to shift the phytoplankton community towards dominance of smaller-sized species due to global warming. Yet, little is known about their viral mortality agents despite the ecological importance of viruses regulating phytoplankton host dynamics and diversity. Here we report the isolation and basic characterization of four prasinoviruses infectious to the common Arctic picophytoplankter Micromonas. We furthermore assessed how temperature influenced viral infectivity and production. Phylogenetic analysis indicated that the putative double-stranded DNA (dsDNA) Micromonas polaris viruses (MpoVs) are prasinoviruses (Phycodnaviridae) of approximately 120 nm in particle size. One MpoV showed intrinsic differences to the other three viruses, i.e., larger genome size (205 ± 2 vs. 191 ± 3 Kb), broader host range, and longer latent period (39 vs. 18 h). Temperature increase shortened the latent periods (up to 50%), increased the burst size (up to 40%), and affected viral infectivity. However, the variability in response to temperature was high for the different viruses and host strains assessed, likely affecting the Arctic picoeukaryote community structure both in the short term (seasonal cycles) and long term (global warming). Article in Journal/Newspaper Arctic Arctic Climate change Global warming Phytoplankton Natural Environment Research Council: NERC Open Research Archive Arctic Viruses 9 6 134 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Global climate change-induced warming of the Artic seas is predicted to shift the phytoplankton community towards dominance of smaller-sized species due to global warming. Yet, little is known about their viral mortality agents despite the ecological importance of viruses regulating phytoplankton host dynamics and diversity. Here we report the isolation and basic characterization of four prasinoviruses infectious to the common Arctic picophytoplankter Micromonas. We furthermore assessed how temperature influenced viral infectivity and production. Phylogenetic analysis indicated that the putative double-stranded DNA (dsDNA) Micromonas polaris viruses (MpoVs) are prasinoviruses (Phycodnaviridae) of approximately 120 nm in particle size. One MpoV showed intrinsic differences to the other three viruses, i.e., larger genome size (205 ± 2 vs. 191 ± 3 Kb), broader host range, and longer latent period (39 vs. 18 h). Temperature increase shortened the latent periods (up to 50%), increased the burst size (up to 40%), and affected viral infectivity. However, the variability in response to temperature was high for the different viruses and host strains assessed, likely affecting the Arctic picoeukaryote community structure both in the short term (seasonal cycles) and long term (global warming). |
format |
Article in Journal/Newspaper |
author |
Maat, Douwe S. Biggs, Tristan Evans, Claire van Bleijswijk, Judith D.L. van der Wel, Nicole N. Dutilh, Bas E. Brussaard, Corina P.D. |
spellingShingle |
Maat, Douwe S. Biggs, Tristan Evans, Claire van Bleijswijk, Judith D.L. van der Wel, Nicole N. Dutilh, Bas E. Brussaard, Corina P.D. Characterization and temperature dependence of Arctic Micromonas polaris viruses |
author_facet |
Maat, Douwe S. Biggs, Tristan Evans, Claire van Bleijswijk, Judith D.L. van der Wel, Nicole N. Dutilh, Bas E. Brussaard, Corina P.D. |
author_sort |
Maat, Douwe S. |
title |
Characterization and temperature dependence of Arctic Micromonas polaris viruses |
title_short |
Characterization and temperature dependence of Arctic Micromonas polaris viruses |
title_full |
Characterization and temperature dependence of Arctic Micromonas polaris viruses |
title_fullStr |
Characterization and temperature dependence of Arctic Micromonas polaris viruses |
title_full_unstemmed |
Characterization and temperature dependence of Arctic Micromonas polaris viruses |
title_sort |
characterization and temperature dependence of arctic micromonas polaris viruses |
publishDate |
2017 |
url |
http://nora.nerc.ac.uk/id/eprint/517558/ https://nora.nerc.ac.uk/id/eprint/517558/1/viruses-09-00134-v4.pdf https://doi.org/10.3390/v9060134 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic Climate change Global warming Phytoplankton |
genre_facet |
Arctic Arctic Climate change Global warming Phytoplankton |
op_relation |
https://nora.nerc.ac.uk/id/eprint/517558/1/viruses-09-00134-v4.pdf Maat, Douwe S.; Biggs, Tristan; Evans, Claire orcid:0000-0003-0569-7057 van Bleijswijk, Judith D.L.; van der Wel, Nicole N.; Dutilh, Bas E.; Brussaard, Corina P.D. 2017 Characterization and temperature dependence of Arctic Micromonas polaris viruses. Viruses, 9 (6). 134. https://doi.org/10.3390/v9060134 <https://doi.org/10.3390/v9060134> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/v9060134 |
container_title |
Viruses |
container_volume |
9 |
container_issue |
6 |
container_start_page |
134 |
_version_ |
1766300153013075968 |