Biodegradation of phenol by cold-adapted bacteria from Antarctic soils
Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is a great challenge due to the harsh environmental conditions. To date, there is little information available concerning the biodegradation of phenol by indigenous Antarctic b...
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Online Access: | http://nora.nerc.ac.uk/id/eprint/515984/ https://nora.nerc.ac.uk/id/eprint/515984/1/Lee%20et%20al%20%28Revised%20manuscript%29%20PB%2025.11.17%20%282%29.docx https://nora.nerc.ac.uk/id/eprint/515984/2/Lee%20et%20al%20%28Electronic%20Supplementary%20Material%29%20PB%2015.08.2017.docx https://nora.nerc.ac.uk/id/eprint/515984/3/Lee%20et%20al%20%28Revised%20Figures%29%20PB%2015.08.17.docx https://link.springer.com/article/10.1007/s00300-017-2216-y |
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ftnerc:oai:nora.nerc.ac.uk:515984 2023-05-15T13:49:33+02:00 Biodegradation of phenol by cold-adapted bacteria from Antarctic soils Lee, Gillian Li Yin Ahmad, Siti Aqlima Yasid, Nur Adeela Zulkharnain, Azham Convey, Peter Johari, Wan Lutfi Wan Alias, Siti Aisyah Gonzalez-Rocha, Gerardo Shukor, Mohd Yunus 2018-03 text http://nora.nerc.ac.uk/id/eprint/515984/ https://nora.nerc.ac.uk/id/eprint/515984/1/Lee%20et%20al%20%28Revised%20manuscript%29%20PB%2025.11.17%20%282%29.docx https://nora.nerc.ac.uk/id/eprint/515984/2/Lee%20et%20al%20%28Electronic%20Supplementary%20Material%29%20PB%2015.08.2017.docx https://nora.nerc.ac.uk/id/eprint/515984/3/Lee%20et%20al%20%28Revised%20Figures%29%20PB%2015.08.17.docx https://link.springer.com/article/10.1007/s00300-017-2216-y en eng Springer https://nora.nerc.ac.uk/id/eprint/515984/1/Lee%20et%20al%20%28Revised%20manuscript%29%20PB%2025.11.17%20%282%29.docx https://nora.nerc.ac.uk/id/eprint/515984/2/Lee%20et%20al%20%28Electronic%20Supplementary%20Material%29%20PB%2015.08.2017.docx https://nora.nerc.ac.uk/id/eprint/515984/3/Lee%20et%20al%20%28Revised%20Figures%29%20PB%2015.08.17.docx Lee, Gillian Li Yin; Ahmad, Siti Aqlima; Yasid, Nur Adeela; Zulkharnain, Azham; Convey, Peter orcid:0000-0001-8497-9903 Johari, Wan Lutfi Wan; Alias, Siti Aisyah; Gonzalez-Rocha, Gerardo; Shukor, Mohd Yunus. 2018 Biodegradation of phenol by cold-adapted bacteria from Antarctic soils. Polar Biology, 41 (3). 553-562. https://doi.org/10.1007/s00300-017-2216-y <https://doi.org/10.1007/s00300-017-2216-y> Publication - Article PeerReviewed 2018 ftnerc https://doi.org/10.1007/s00300-017-2216-y 2023-02-04T19:44:20Z Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is a great challenge due to the harsh environmental conditions. To date, there is little information available concerning the biodegradation of phenol by indigenous Antarctic bacteria. This study addresses the isolation of three phenol-degrading bacterial strains from King George Island, Antarctica. Based on preliminary screening, three isolates (AQ5-05, AQ5-06 and AQ5-07) capable of completely degrading 0.5 g/L phenol within 120 h at 10 °C were selected for detailed study. Two were identified as Arthrobacter spp., and one Rhodococcus sp., based on 16S rRNA sequences. All strains were non-motile, Gram positive, oxidase negative and catalase positive. A study on the effects of parameters including temperature, pH, salinity and nitrogen source was conducted to optimise the conditions for phenol degradation. This revealed that the three isolates were psychrotolerant with the optimum temperature for phenol degradation between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol over a wide range of low temperatures. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Polar Biology Natural Environment Research Council: NERC Open Research Archive Antarctic King George Island Polar Biology 41 3 553 562 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is a great challenge due to the harsh environmental conditions. To date, there is little information available concerning the biodegradation of phenol by indigenous Antarctic bacteria. This study addresses the isolation of three phenol-degrading bacterial strains from King George Island, Antarctica. Based on preliminary screening, three isolates (AQ5-05, AQ5-06 and AQ5-07) capable of completely degrading 0.5 g/L phenol within 120 h at 10 °C were selected for detailed study. Two were identified as Arthrobacter spp., and one Rhodococcus sp., based on 16S rRNA sequences. All strains were non-motile, Gram positive, oxidase negative and catalase positive. A study on the effects of parameters including temperature, pH, salinity and nitrogen source was conducted to optimise the conditions for phenol degradation. This revealed that the three isolates were psychrotolerant with the optimum temperature for phenol degradation between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol over a wide range of low temperatures. |
format |
Article in Journal/Newspaper |
author |
Lee, Gillian Li Yin Ahmad, Siti Aqlima Yasid, Nur Adeela Zulkharnain, Azham Convey, Peter Johari, Wan Lutfi Wan Alias, Siti Aisyah Gonzalez-Rocha, Gerardo Shukor, Mohd Yunus |
spellingShingle |
Lee, Gillian Li Yin Ahmad, Siti Aqlima Yasid, Nur Adeela Zulkharnain, Azham Convey, Peter Johari, Wan Lutfi Wan Alias, Siti Aisyah Gonzalez-Rocha, Gerardo Shukor, Mohd Yunus Biodegradation of phenol by cold-adapted bacteria from Antarctic soils |
author_facet |
Lee, Gillian Li Yin Ahmad, Siti Aqlima Yasid, Nur Adeela Zulkharnain, Azham Convey, Peter Johari, Wan Lutfi Wan Alias, Siti Aisyah Gonzalez-Rocha, Gerardo Shukor, Mohd Yunus |
author_sort |
Lee, Gillian Li Yin |
title |
Biodegradation of phenol by cold-adapted bacteria from Antarctic soils |
title_short |
Biodegradation of phenol by cold-adapted bacteria from Antarctic soils |
title_full |
Biodegradation of phenol by cold-adapted bacteria from Antarctic soils |
title_fullStr |
Biodegradation of phenol by cold-adapted bacteria from Antarctic soils |
title_full_unstemmed |
Biodegradation of phenol by cold-adapted bacteria from Antarctic soils |
title_sort |
biodegradation of phenol by cold-adapted bacteria from antarctic soils |
publisher |
Springer |
publishDate |
2018 |
url |
http://nora.nerc.ac.uk/id/eprint/515984/ https://nora.nerc.ac.uk/id/eprint/515984/1/Lee%20et%20al%20%28Revised%20manuscript%29%20PB%2025.11.17%20%282%29.docx https://nora.nerc.ac.uk/id/eprint/515984/2/Lee%20et%20al%20%28Electronic%20Supplementary%20Material%29%20PB%2015.08.2017.docx https://nora.nerc.ac.uk/id/eprint/515984/3/Lee%20et%20al%20%28Revised%20Figures%29%20PB%2015.08.17.docx https://link.springer.com/article/10.1007/s00300-017-2216-y |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island Polar Biology |
genre_facet |
Antarc* Antarctic Antarctica King George Island Polar Biology |
op_relation |
https://nora.nerc.ac.uk/id/eprint/515984/1/Lee%20et%20al%20%28Revised%20manuscript%29%20PB%2025.11.17%20%282%29.docx https://nora.nerc.ac.uk/id/eprint/515984/2/Lee%20et%20al%20%28Electronic%20Supplementary%20Material%29%20PB%2015.08.2017.docx https://nora.nerc.ac.uk/id/eprint/515984/3/Lee%20et%20al%20%28Revised%20Figures%29%20PB%2015.08.17.docx Lee, Gillian Li Yin; Ahmad, Siti Aqlima; Yasid, Nur Adeela; Zulkharnain, Azham; Convey, Peter orcid:0000-0001-8497-9903 Johari, Wan Lutfi Wan; Alias, Siti Aisyah; Gonzalez-Rocha, Gerardo; Shukor, Mohd Yunus. 2018 Biodegradation of phenol by cold-adapted bacteria from Antarctic soils. Polar Biology, 41 (3). 553-562. https://doi.org/10.1007/s00300-017-2216-y <https://doi.org/10.1007/s00300-017-2216-y> |
op_doi |
https://doi.org/10.1007/s00300-017-2216-y |
container_title |
Polar Biology |
container_volume |
41 |
container_issue |
3 |
container_start_page |
553 |
op_container_end_page |
562 |
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1766251639883169792 |