AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp.
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteri...
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Nature Research
2017
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Online Access: | http://nora.nerc.ac.uk/id/eprint/514831/ https://nora.nerc.ac.uk/id/eprint/514831/1/srep42968.pdf http://www.nature.com/articles/srep42968 |
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ftnerc:oai:nora.nerc.ac.uk:514831 2024-02-11T09:56:37+01:00 AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan 2017-02-22 text http://nora.nerc.ac.uk/id/eprint/514831/ https://nora.nerc.ac.uk/id/eprint/514831/1/srep42968.pdf http://www.nature.com/articles/srep42968 en eng Nature Research https://nora.nerc.ac.uk/id/eprint/514831/1/srep42968.pdf See-Too, Wah Seng; Ee, Robson; Lim, Yan-Lue; Convey, Peter orcid:0000-0001-8497-9903 Pearce, David orcid:0000-0001-5292-4596 Yin, Wai-Fong; Chan, Kok-Gan. 2017 AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. Scientific Reports, 7, 42968. https://doi.org/10.1038/srep42968 <https://doi.org/10.1038/srep42968> cc_by_4 Publication - Article PeerReviewed 2017 ftnerc https://doi.org/10.1038/srep42968 2024-01-19T00:03:13Z Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This study is the first report on QQ activity of bacteria isolated from the polar regions. Article in Journal/Newspaper Antarc* Antarctic Antarctica Elephant Seal Natural Environment Research Council: NERC Open Research Archive Antarctic Scientific Reports 7 1 |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
English |
description |
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This study is the first report on QQ activity of bacteria isolated from the polar regions. |
format |
Article in Journal/Newspaper |
author |
See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan |
spellingShingle |
See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. |
author_facet |
See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan |
author_sort |
See-Too, Wah Seng |
title |
AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. |
title_short |
AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. |
title_full |
AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. |
title_fullStr |
AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. |
title_full_unstemmed |
AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. |
title_sort |
aidp, a novel n-acyl homoserine lactonase gene from antarctic planococcus sp. |
publisher |
Nature Research |
publishDate |
2017 |
url |
http://nora.nerc.ac.uk/id/eprint/514831/ https://nora.nerc.ac.uk/id/eprint/514831/1/srep42968.pdf http://www.nature.com/articles/srep42968 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Elephant Seal |
genre_facet |
Antarc* Antarctic Antarctica Elephant Seal |
op_relation |
https://nora.nerc.ac.uk/id/eprint/514831/1/srep42968.pdf See-Too, Wah Seng; Ee, Robson; Lim, Yan-Lue; Convey, Peter orcid:0000-0001-8497-9903 Pearce, David orcid:0000-0001-5292-4596 Yin, Wai-Fong; Chan, Kok-Gan. 2017 AidP, a novel N-Acyl Homoserine Lactonase gene from Antarctic Planococcus sp. Scientific Reports, 7, 42968. https://doi.org/10.1038/srep42968 <https://doi.org/10.1038/srep42968> |
op_rights |
cc_by_4 |
op_doi |
https://doi.org/10.1038/srep42968 |
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Scientific Reports |
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7 |
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1 |
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1790604511328337920 |