Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing
Differential scanning calorimetry was used to characterize thermal events associated with freezing and melting of suspensions and extracts of Panagrolaimus davidi, an Antarctic nematode which can survive intracellular freezing. Nematode suspensions produced a single freezing exotherm with a shoulder...
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Academic Press
1997
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Online Access: | http://nora.nerc.ac.uk/id/eprint/514777/ https://doi.org/10.1006/cryo.1996.1989 |
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ftnerc:oai:nora.nerc.ac.uk:514777 2023-05-15T13:49:33+02:00 Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing Wharton, David A. Block, William 1997 http://nora.nerc.ac.uk/id/eprint/514777/ https://doi.org/10.1006/cryo.1996.1989 unknown Academic Press Wharton, David A.; Block, William. 1997 Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing. Cryobiology, 34 (2). 114-121. https://doi.org/10.1006/cryo.1996.1989 <https://doi.org/10.1006/cryo.1996.1989> Publication - Article PeerReviewed 1997 ftnerc https://doi.org/10.1006/cryo.1996.1989 2023-02-04T19:43:40Z Differential scanning calorimetry was used to characterize thermal events associated with freezing and melting of suspensions and extracts of Panagrolaimus davidi, an Antarctic nematode which can survive intracellular freezing. Nematode suspensions produced a single freezing exotherm with a shoulder on the peak representing the freezing of the nematodes. A shoulder on the peak of melting endotherms indicates the melting of the nematodes and of the water surrounding them. Exotherms were also detected from individual nematodes mounted in liquid paraffin. The freezing of nematodes was very rapid and in marked contrast to that of freezing-tolerant insects and vertebrates, which take hours or days to freeze. Eighty-two percent of the nematodes' body water froze. High levels of survival were obtained in nematodes exposed to temperatures down to -40 degrees C. No additional thermal events were observed after the freezing event and before the melting of samples cooled to -40 degrees C, indicating no changes in the proportion of body water frozen. Ice nucleating activity is present in nematode suspensions but not in supernatants from nematode extracts. No thermal hysteresis activity was detected in nematode extracts. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic Cryobiology 34 2 114 121 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
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unknown |
description |
Differential scanning calorimetry was used to characterize thermal events associated with freezing and melting of suspensions and extracts of Panagrolaimus davidi, an Antarctic nematode which can survive intracellular freezing. Nematode suspensions produced a single freezing exotherm with a shoulder on the peak representing the freezing of the nematodes. A shoulder on the peak of melting endotherms indicates the melting of the nematodes and of the water surrounding them. Exotherms were also detected from individual nematodes mounted in liquid paraffin. The freezing of nematodes was very rapid and in marked contrast to that of freezing-tolerant insects and vertebrates, which take hours or days to freeze. Eighty-two percent of the nematodes' body water froze. High levels of survival were obtained in nematodes exposed to temperatures down to -40 degrees C. No additional thermal events were observed after the freezing event and before the melting of samples cooled to -40 degrees C, indicating no changes in the proportion of body water frozen. Ice nucleating activity is present in nematode suspensions but not in supernatants from nematode extracts. No thermal hysteresis activity was detected in nematode extracts. |
format |
Article in Journal/Newspaper |
author |
Wharton, David A. Block, William |
spellingShingle |
Wharton, David A. Block, William Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing |
author_facet |
Wharton, David A. Block, William |
author_sort |
Wharton, David A. |
title |
Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing |
title_short |
Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing |
title_full |
Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing |
title_fullStr |
Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing |
title_full_unstemmed |
Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing |
title_sort |
differential scanning calorimetry studies on an antarctic nematode (panagrolaimus davidi) which survives intracellular freezing |
publisher |
Academic Press |
publishDate |
1997 |
url |
http://nora.nerc.ac.uk/id/eprint/514777/ https://doi.org/10.1006/cryo.1996.1989 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Wharton, David A.; Block, William. 1997 Differential scanning calorimetry studies on an Antarctic nematode (Panagrolaimus davidi) which survives intracellular freezing. Cryobiology, 34 (2). 114-121. https://doi.org/10.1006/cryo.1996.1989 <https://doi.org/10.1006/cryo.1996.1989> |
op_doi |
https://doi.org/10.1006/cryo.1996.1989 |
container_title |
Cryobiology |
container_volume |
34 |
container_issue |
2 |
container_start_page |
114 |
op_container_end_page |
121 |
_version_ |
1766251569116872704 |