An Antarctic molluscan biomineralisation tool-kit
The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, p...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Publishing Group
2016
|
Subjects: | |
Online Access: | http://nora.nerc.ac.uk/id/eprint/513908/ https://nora.nerc.ac.uk/id/eprint/513908/1/srep36978.pdf http://www.nature.com/articles/srep36978 |
id |
ftnerc:oai:nora.nerc.ac.uk:513908 |
---|---|
record_format |
openpolar |
spelling |
ftnerc:oai:nora.nerc.ac.uk:513908 2023-05-15T13:49:32+02:00 An Antarctic molluscan biomineralisation tool-kit Sleight, Victoria A. Marie, Benjamin Jackson, Daniel J. Dyrynda, Elizabeth A. Marie, Arul Clark, Melody S. 2016-11 text http://nora.nerc.ac.uk/id/eprint/513908/ https://nora.nerc.ac.uk/id/eprint/513908/1/srep36978.pdf http://www.nature.com/articles/srep36978 en eng Nature Publishing Group https://nora.nerc.ac.uk/id/eprint/513908/1/srep36978.pdf Sleight, Victoria A. orcid:0000-0003-0550-8500 Marie, Benjamin; Jackson, Daniel J.; Dyrynda, Elizabeth A.; Marie, Arul; Clark, Melody S. orcid:0000-0002-3442-3824 . 2016 An Antarctic molluscan biomineralisation tool-kit. Scientific Reports, 6, 36978. 13, pp. https://doi.org/10.1038/srep36978 <https://doi.org/10.1038/srep36978> cc_by_4 CC-BY Publication - Article PeerReviewed 2016 ftnerc https://doi.org/10.1038/srep36978 2023-02-04T19:43:14Z The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, proteomics and gene expression to investigate this process. Thirty seven proteins were identified via proteomic extraction of the nacreous shell layer, including two not previously found in nacre; a novel T-rich Mucin-like protein and a Zinc-dependent metalloprotease. In situ hybridisation of seven candidate biomineralisation genes revealed discrete spatial expression patterns within the mantle tissue, hinting at modular organisation, which is also observed in the mantle tissues of other molluscs. All seven of these biomineralisation candidates displayed evidence of multifunctionality and strong association with vesicles, which are potentially involved in shell secretion in this species. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic The Antarctic Scientific Reports 6 1 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, proteomics and gene expression to investigate this process. Thirty seven proteins were identified via proteomic extraction of the nacreous shell layer, including two not previously found in nacre; a novel T-rich Mucin-like protein and a Zinc-dependent metalloprotease. In situ hybridisation of seven candidate biomineralisation genes revealed discrete spatial expression patterns within the mantle tissue, hinting at modular organisation, which is also observed in the mantle tissues of other molluscs. All seven of these biomineralisation candidates displayed evidence of multifunctionality and strong association with vesicles, which are potentially involved in shell secretion in this species. |
format |
Article in Journal/Newspaper |
author |
Sleight, Victoria A. Marie, Benjamin Jackson, Daniel J. Dyrynda, Elizabeth A. Marie, Arul Clark, Melody S. |
spellingShingle |
Sleight, Victoria A. Marie, Benjamin Jackson, Daniel J. Dyrynda, Elizabeth A. Marie, Arul Clark, Melody S. An Antarctic molluscan biomineralisation tool-kit |
author_facet |
Sleight, Victoria A. Marie, Benjamin Jackson, Daniel J. Dyrynda, Elizabeth A. Marie, Arul Clark, Melody S. |
author_sort |
Sleight, Victoria A. |
title |
An Antarctic molluscan biomineralisation tool-kit |
title_short |
An Antarctic molluscan biomineralisation tool-kit |
title_full |
An Antarctic molluscan biomineralisation tool-kit |
title_fullStr |
An Antarctic molluscan biomineralisation tool-kit |
title_full_unstemmed |
An Antarctic molluscan biomineralisation tool-kit |
title_sort |
antarctic molluscan biomineralisation tool-kit |
publisher |
Nature Publishing Group |
publishDate |
2016 |
url |
http://nora.nerc.ac.uk/id/eprint/513908/ https://nora.nerc.ac.uk/id/eprint/513908/1/srep36978.pdf http://www.nature.com/articles/srep36978 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://nora.nerc.ac.uk/id/eprint/513908/1/srep36978.pdf Sleight, Victoria A. orcid:0000-0003-0550-8500 Marie, Benjamin; Jackson, Daniel J.; Dyrynda, Elizabeth A.; Marie, Arul; Clark, Melody S. orcid:0000-0002-3442-3824 . 2016 An Antarctic molluscan biomineralisation tool-kit. Scientific Reports, 6, 36978. 13, pp. https://doi.org/10.1038/srep36978 <https://doi.org/10.1038/srep36978> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/srep36978 |
container_title |
Scientific Reports |
container_volume |
6 |
container_issue |
1 |
_version_ |
1766251535183904768 |