Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata
Members of the Myidae family are ecologically and economically important, but there is currently very little molecular data on these species. The present study sequenced and assembled the mantle transcriptome of Mya truncata from the North West coast of Scotland and identified candidate biomineralis...
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Online Access: | http://nora.nerc.ac.uk/id/eprint/512717/ https://nora.nerc.ac.uk/id/eprint/512717/1/Sleight.pdf https://doi.org/10.1016/j.margen.2016.01.003 |
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ftnerc:oai:nora.nerc.ac.uk:512717 2023-05-15T13:49:32+02:00 Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata Sleight, Victoria A. Thorne, Michael A.S. Peck, Lloyd S. Arivalagan, Jaison Berland, Sophie Marie, Arul Clark, Melody S. 2016-01-14 text http://nora.nerc.ac.uk/id/eprint/512717/ https://nora.nerc.ac.uk/id/eprint/512717/1/Sleight.pdf https://doi.org/10.1016/j.margen.2016.01.003 en eng Elsevier https://nora.nerc.ac.uk/id/eprint/512717/1/Sleight.pdf Sleight, Victoria A. orcid:0000-0003-0550-8500 Thorne, Michael A.S. orcid:0000-0001-7759-612X Peck, Lloyd S. orcid:0000-0003-3479-6791 Arivalagan, Jaison; Berland, Sophie; Marie, Arul; Clark, Melody S. orcid:0000-0002-3442-3824 . 2016 Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata. Marine Genomics, 27. 47-55. https://doi.org/10.1016/j.margen.2016.01.003 <https://doi.org/10.1016/j.margen.2016.01.003> cc_by_4 CC-BY Publication - Article PeerReviewed 2016 ftnerc https://doi.org/10.1016/j.margen.2016.01.003 2023-02-04T19:42:35Z Members of the Myidae family are ecologically and economically important, but there is currently very little molecular data on these species. The present study sequenced and assembled the mantle transcriptome of Mya truncata from the North West coast of Scotland and identified candidate biomineralisation genes. RNA-Seq reads were assembled to create 20,106 contigs in a de novo transciptome, 18.81% of which were assigned putative functions using BLAST sequence similarity searching (cuttoff E-value 1E − 10). The most highly expressed genes were compared to the Antarctic clam (Laternula elliptica) and showed that many of the dominant biological functions (muscle contraction, energy production, biomineralisation) in the mantle were conserved. There were however, differences in the constitutive expression of heat shock proteins, which were possibly due to the M. truncata sampling location being at a relatively low latitude, and hence relatively warm, in terms of the global distribution of the species. Phylogenetic analyses of the Tyrosinase proteins from M. truncata showed a gene expansion which was absent in L. elliptica. The tissue distribution expression patterns of putative biomineralisation genes were investigated using quantitative PCR, all genes showed a mantle specific expression pattern supporting their hypothesised role in shell secretion. The present study provides some preliminary insights into how clams from different environments – temperate versus polar – build their shells. In addition, the transcriptome data provides a valuable resource for future comparative studies investigating biomineralisation. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic The Antarctic Marine Genomics 27 47 55 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
description |
Members of the Myidae family are ecologically and economically important, but there is currently very little molecular data on these species. The present study sequenced and assembled the mantle transcriptome of Mya truncata from the North West coast of Scotland and identified candidate biomineralisation genes. RNA-Seq reads were assembled to create 20,106 contigs in a de novo transciptome, 18.81% of which were assigned putative functions using BLAST sequence similarity searching (cuttoff E-value 1E − 10). The most highly expressed genes were compared to the Antarctic clam (Laternula elliptica) and showed that many of the dominant biological functions (muscle contraction, energy production, biomineralisation) in the mantle were conserved. There were however, differences in the constitutive expression of heat shock proteins, which were possibly due to the M. truncata sampling location being at a relatively low latitude, and hence relatively warm, in terms of the global distribution of the species. Phylogenetic analyses of the Tyrosinase proteins from M. truncata showed a gene expansion which was absent in L. elliptica. The tissue distribution expression patterns of putative biomineralisation genes were investigated using quantitative PCR, all genes showed a mantle specific expression pattern supporting their hypothesised role in shell secretion. The present study provides some preliminary insights into how clams from different environments – temperate versus polar – build their shells. In addition, the transcriptome data provides a valuable resource for future comparative studies investigating biomineralisation. |
format |
Article in Journal/Newspaper |
author |
Sleight, Victoria A. Thorne, Michael A.S. Peck, Lloyd S. Arivalagan, Jaison Berland, Sophie Marie, Arul Clark, Melody S. |
spellingShingle |
Sleight, Victoria A. Thorne, Michael A.S. Peck, Lloyd S. Arivalagan, Jaison Berland, Sophie Marie, Arul Clark, Melody S. Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata |
author_facet |
Sleight, Victoria A. Thorne, Michael A.S. Peck, Lloyd S. Arivalagan, Jaison Berland, Sophie Marie, Arul Clark, Melody S. |
author_sort |
Sleight, Victoria A. |
title |
Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata |
title_short |
Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata |
title_full |
Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata |
title_fullStr |
Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata |
title_full_unstemmed |
Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata |
title_sort |
characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, mya truncata |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://nora.nerc.ac.uk/id/eprint/512717/ https://nora.nerc.ac.uk/id/eprint/512717/1/Sleight.pdf https://doi.org/10.1016/j.margen.2016.01.003 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://nora.nerc.ac.uk/id/eprint/512717/1/Sleight.pdf Sleight, Victoria A. orcid:0000-0003-0550-8500 Thorne, Michael A.S. orcid:0000-0001-7759-612X Peck, Lloyd S. orcid:0000-0003-3479-6791 Arivalagan, Jaison; Berland, Sophie; Marie, Arul; Clark, Melody S. orcid:0000-0002-3442-3824 . 2016 Characterisation of the mantle transcriptome and biomineralisation genes in the blunt-gaper clam, Mya truncata. Marine Genomics, 27. 47-55. https://doi.org/10.1016/j.margen.2016.01.003 <https://doi.org/10.1016/j.margen.2016.01.003> |
op_rights |
cc_by_4 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.margen.2016.01.003 |
container_title |
Marine Genomics |
container_volume |
27 |
container_start_page |
47 |
op_container_end_page |
55 |
_version_ |
1766251519412273152 |