Antimicrobial activity of microfungi from maritime Antarctic soil
The search for cold-adapted and cold-active fungi in extreme environments provides the potential for discovering new species and novel bioactive compounds. In this study, soil samples were collected from Deception Island, Wilhelmina Bay (north-west Antarctic Peninsula, Graham Land) and Yankee Bay (G...
Published in: | Czech Polar Reports |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
muniPress Masaryk University Brno
2016
|
Subjects: | |
Online Access: | http://nora.nerc.ac.uk/id/eprint/504449/ http://www.sci.muni.cz/CPR/ |
id |
ftnerc:oai:nora.nerc.ac.uk:504449 |
---|---|
record_format |
openpolar |
spelling |
ftnerc:oai:nora.nerc.ac.uk:504449 2023-05-15T13:48:08+02:00 Antimicrobial activity of microfungi from maritime Antarctic soil Abneuf, Mohammed A. Khrishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Alias, Siti Aisyah 2016-10 http://nora.nerc.ac.uk/id/eprint/504449/ http://www.sci.muni.cz/CPR/ unknown muniPress Masaryk University Brno Abneuf, Mohammed A.; Khrishnan, Abiramy; Aravena, Marcelo Gonzalez; Pang, Ka-Lai; Convey, Peter orcid:0000-0001-8497-9903 Alias, Siti Aisyah. 2016 Antimicrobial activity of microfungi from maritime Antarctic soil. Czech Polar Reports, 6 (2). 141-154. https://doi.org/10.5817/CPR2016-2-13 <https://doi.org/10.5817/CPR2016-2-13> Publication - Article PeerReviewed 2016 ftnerc https://doi.org/10.5817/CPR2016-2-13 2023-02-04T19:38:27Z The search for cold-adapted and cold-active fungi in extreme environments provides the potential for discovering new species and novel bioactive compounds. In this study, soil samples were collected from Deception Island, Wilhelmina Bay (north-west Antarctic Peninsula, Graham Land) and Yankee Bay (Greenwich Island), maritime Antarctica, for the isolation of soil fungi and determination of their antimicrobial activity. The soil-plate method, agar block, disc diffusion and broth micro-dilution assays were applied to characterize the thermal classes and antimicrobial activity of the isolated fungi. A total of 27 isolates of fungi were obtained from 14 soil samples, including 13 Ascomycota, 4 Zygomycota and 10 anamorphic fungi. Cold-active (psychrotolerant) fungi predominated over cold-adapted (psychrophilic) fungi. In the antimicrobial assay, 16 isolates showed substantial inhibitory activity against test bacterial pathogens. Ethyl acetate extracts of 10 competent isolates showed significant inhibition of bacterial pathogens. Antifungal activity was observed in the disc diffusion assay, but not in the agar block assay. Minimum inhibitory, bactericidal and fungicidal concentrations were determined using the broth micro-dilution method, with an average in the range of 0.78-25 mg ml-1 on the test microorganisms. Isolate WHB-sp. 7 showed the best broad spectrum antimicrobial activity, with the potential for biotechnological studies in antibiotic development. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island Graham Land Greenwich Island Natural Environment Research Council: NERC Open Research Archive Antarctic Antarctic Peninsula Greenwich Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Graham Land ENVELOPE(-63.500,-63.500,-66.000,-66.000) Yankee ENVELOPE(-59.769,-59.769,-62.526,-62.526) Wilhelmina ENVELOPE(-62.160,-62.160,-64.642,-64.642) Greenwich Island ENVELOPE(-59.783,-59.783,-62.517,-62.517) Wilhelmina Bay ENVELOPE(-62.160,-62.160,-64.642,-64.642) Czech Polar Reports 6 2 141 154 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
unknown |
description |
The search for cold-adapted and cold-active fungi in extreme environments provides the potential for discovering new species and novel bioactive compounds. In this study, soil samples were collected from Deception Island, Wilhelmina Bay (north-west Antarctic Peninsula, Graham Land) and Yankee Bay (Greenwich Island), maritime Antarctica, for the isolation of soil fungi and determination of their antimicrobial activity. The soil-plate method, agar block, disc diffusion and broth micro-dilution assays were applied to characterize the thermal classes and antimicrobial activity of the isolated fungi. A total of 27 isolates of fungi were obtained from 14 soil samples, including 13 Ascomycota, 4 Zygomycota and 10 anamorphic fungi. Cold-active (psychrotolerant) fungi predominated over cold-adapted (psychrophilic) fungi. In the antimicrobial assay, 16 isolates showed substantial inhibitory activity against test bacterial pathogens. Ethyl acetate extracts of 10 competent isolates showed significant inhibition of bacterial pathogens. Antifungal activity was observed in the disc diffusion assay, but not in the agar block assay. Minimum inhibitory, bactericidal and fungicidal concentrations were determined using the broth micro-dilution method, with an average in the range of 0.78-25 mg ml-1 on the test microorganisms. Isolate WHB-sp. 7 showed the best broad spectrum antimicrobial activity, with the potential for biotechnological studies in antibiotic development. |
format |
Article in Journal/Newspaper |
author |
Abneuf, Mohammed A. Khrishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Alias, Siti Aisyah |
spellingShingle |
Abneuf, Mohammed A. Khrishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Alias, Siti Aisyah Antimicrobial activity of microfungi from maritime Antarctic soil |
author_facet |
Abneuf, Mohammed A. Khrishnan, Abiramy Aravena, Marcelo Gonzalez Pang, Ka-Lai Convey, Peter Alias, Siti Aisyah |
author_sort |
Abneuf, Mohammed A. |
title |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_short |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_full |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_fullStr |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_full_unstemmed |
Antimicrobial activity of microfungi from maritime Antarctic soil |
title_sort |
antimicrobial activity of microfungi from maritime antarctic soil |
publisher |
muniPress Masaryk University Brno |
publishDate |
2016 |
url |
http://nora.nerc.ac.uk/id/eprint/504449/ http://www.sci.muni.cz/CPR/ |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(-63.500,-63.500,-66.000,-66.000) ENVELOPE(-59.769,-59.769,-62.526,-62.526) ENVELOPE(-62.160,-62.160,-64.642,-64.642) ENVELOPE(-59.783,-59.783,-62.517,-62.517) ENVELOPE(-62.160,-62.160,-64.642,-64.642) |
geographic |
Antarctic Antarctic Peninsula Greenwich Deception Island Graham Land Yankee Wilhelmina Greenwich Island Wilhelmina Bay |
geographic_facet |
Antarctic Antarctic Peninsula Greenwich Deception Island Graham Land Yankee Wilhelmina Greenwich Island Wilhelmina Bay |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island Graham Land Greenwich Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Deception Island Graham Land Greenwich Island |
op_relation |
Abneuf, Mohammed A.; Khrishnan, Abiramy; Aravena, Marcelo Gonzalez; Pang, Ka-Lai; Convey, Peter orcid:0000-0001-8497-9903 Alias, Siti Aisyah. 2016 Antimicrobial activity of microfungi from maritime Antarctic soil. Czech Polar Reports, 6 (2). 141-154. https://doi.org/10.5817/CPR2016-2-13 <https://doi.org/10.5817/CPR2016-2-13> |
op_doi |
https://doi.org/10.5817/CPR2016-2-13 |
container_title |
Czech Polar Reports |
container_volume |
6 |
container_issue |
2 |
container_start_page |
141 |
op_container_end_page |
154 |
_version_ |
1766248733264052224 |