Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias
Background The Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and, in particular, anticipat...
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ftnerc:oai:nora.nerc.ac.uk:500361 2023-05-15T13:48:07+02:00 Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias Toullec, Jean-Yves Corre, Erwan Bernay, Benoît Thorne, Michael A.S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. 2013-08-21 text http://nora.nerc.ac.uk/id/eprint/500361/ https://nora.nerc.ac.uk/id/eprint/500361/1/journal.pone.0071609.pdf en eng https://nora.nerc.ac.uk/id/eprint/500361/1/journal.pone.0071609.pdf Toullec, Jean-Yves; Corre, Erwan; Bernay, Benoît; Thorne, Michael A.S. orcid:0000-0001-7759-612X Cascella, Kévin; Ollivaux, Céline; Henry, Joël; Clark, Melody S. orcid:0000-0002-3442-3824 . 2013 Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias. PLoS One, 8 (8). e71609. https://doi.org/10.1371/journal.pone.0071609 <https://doi.org/10.1371/journal.pone.0071609> cc_by CC-BY Publication - Article PeerReviewed 2013 ftnerc https://doi.org/10.1371/journal.pone.0071609 2023-02-04T19:36:21Z Background The Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and, in particular, anticipate their responses to climate change effects in the warming seas around Antarctica. Methodology/Principal Findings Illumina sequencing was used to produce a transcriptome of the ice krill. Analysis of the assembled contigs via two different methods, produced 36 new pre-pro-peptides, coding for 61 neuropeptides or peptide hormones belonging to the following families: Allatostatins (A, B et C), Bursicon (α and β), Crustacean Hyperglycemic Hormones (CHH and MIH/VIHs), Crustacean Cardioactive Peptide (CCAP), Corazonin, Diuretic Hormones (DH), the Eclosion Hormone (EH), Neuroparsin, Neuropeptide F (NPF), small Neuropeptide F (sNPF), Pigment Dispersing Hormone (PDH), Red Pigment Concentrating Hormone (RPCH) and finally Tachykinin. LC/MS/MS proteomics was also carried out on eyestalk extracts, which are the major site of neuropeptide synthesis in decapod crustaceans. Results confirmed the presence of six neuropeptides and six precursor-related peptides previously identified in the transcriptome analyses. Conclusions This study represents the first comprehensive analysis of neuropeptide hormones in a Eucarida non-decapod Malacostraca, several of which are described for the first time in a non-decapod crustacean. Additionally, there is a potential expansion of PDH and Neuropeptide F family members, which may reflect certain life history traits such as circadian rhythms associated with diurnal migrations and also the confirmation via mass spectrometry of several novel pre-pro-peptides, of unknown function. Knowledge of these essential hormones provides a vital framework for understanding the physiological response of this key Southern Ocean species to climate change and provides a valuable resource for studies into the molecular ... Article in Journal/Newspaper Antarc* Antarctica Southern Ocean Natural Environment Research Council: NERC Open Research Archive Southern Ocean PLoS ONE 8 8 e71609 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
English |
description |
Background The Ice krill, Euphausia crystallorophias is one of the species at the base of the Southern Ocean food chain. Given their significant contribution to the biomass of the Southern Ocean, it is vitally important to gain a better understanding of their physiology and, in particular, anticipate their responses to climate change effects in the warming seas around Antarctica. Methodology/Principal Findings Illumina sequencing was used to produce a transcriptome of the ice krill. Analysis of the assembled contigs via two different methods, produced 36 new pre-pro-peptides, coding for 61 neuropeptides or peptide hormones belonging to the following families: Allatostatins (A, B et C), Bursicon (α and β), Crustacean Hyperglycemic Hormones (CHH and MIH/VIHs), Crustacean Cardioactive Peptide (CCAP), Corazonin, Diuretic Hormones (DH), the Eclosion Hormone (EH), Neuroparsin, Neuropeptide F (NPF), small Neuropeptide F (sNPF), Pigment Dispersing Hormone (PDH), Red Pigment Concentrating Hormone (RPCH) and finally Tachykinin. LC/MS/MS proteomics was also carried out on eyestalk extracts, which are the major site of neuropeptide synthesis in decapod crustaceans. Results confirmed the presence of six neuropeptides and six precursor-related peptides previously identified in the transcriptome analyses. Conclusions This study represents the first comprehensive analysis of neuropeptide hormones in a Eucarida non-decapod Malacostraca, several of which are described for the first time in a non-decapod crustacean. Additionally, there is a potential expansion of PDH and Neuropeptide F family members, which may reflect certain life history traits such as circadian rhythms associated with diurnal migrations and also the confirmation via mass spectrometry of several novel pre-pro-peptides, of unknown function. Knowledge of these essential hormones provides a vital framework for understanding the physiological response of this key Southern Ocean species to climate change and provides a valuable resource for studies into the molecular ... |
format |
Article in Journal/Newspaper |
author |
Toullec, Jean-Yves Corre, Erwan Bernay, Benoît Thorne, Michael A.S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. |
spellingShingle |
Toullec, Jean-Yves Corre, Erwan Bernay, Benoît Thorne, Michael A.S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias |
author_facet |
Toullec, Jean-Yves Corre, Erwan Bernay, Benoît Thorne, Michael A.S. Cascella, Kévin Ollivaux, Céline Henry, Joël Clark, Melody S. |
author_sort |
Toullec, Jean-Yves |
title |
Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias |
title_short |
Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias |
title_full |
Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias |
title_fullStr |
Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias |
title_full_unstemmed |
Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias |
title_sort |
transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a euphausiid: the ice krill euphausia crystallorophias |
publishDate |
2013 |
url |
http://nora.nerc.ac.uk/id/eprint/500361/ https://nora.nerc.ac.uk/id/eprint/500361/1/journal.pone.0071609.pdf |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctica Southern Ocean |
op_relation |
https://nora.nerc.ac.uk/id/eprint/500361/1/journal.pone.0071609.pdf Toullec, Jean-Yves; Corre, Erwan; Bernay, Benoît; Thorne, Michael A.S. orcid:0000-0001-7759-612X Cascella, Kévin; Ollivaux, Céline; Henry, Joël; Clark, Melody S. orcid:0000-0002-3442-3824 . 2013 Transcriptome and peptidome characterisation of the main neuropeptides and peptidic hormones of a Euphausiid: the ice krill Euphausia crystallorophias. PLoS One, 8 (8). e71609. https://doi.org/10.1371/journal.pone.0071609 <https://doi.org/10.1371/journal.pone.0071609> |
op_rights |
cc_by |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0071609 |
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PLoS ONE |
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