Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola)
The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure o...
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ftnerc:oai:nora.nerc.ac.uk:500338 2024-02-11T10:00:48+01:00 Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola) Grubor-Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. 2013-02 http://nora.nerc.ac.uk/id/eprint/500338/ https://doi.org/10.1002/arch.21073 unknown Wiley Grubor-Lajšić, Gordana; Petri, Edward T.; Kojić, Danijela; Purać, Jelena; Popović, Željko D.; Worland, Roger M.; Clark, Melody S. orcid:0000-0002-3442-3824 Mojović, Miloš; Blagojević, Duško P. 2013 Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola). Archives of Insect Biochemistry and Physiology, 82 (2). 59-70. https://doi.org/10.1002/arch.21073 <https://doi.org/10.1002/arch.21073> Publication - Article PeerReviewed 2013 ftnerc https://doi.org/10.1002/arch.21073 2024-01-19T00:03:13Z The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 °C (control) versus −2 °C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 °C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 °C versus 5 °C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 °C (controls) versus −2 °C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 °C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis. Article in Journal/Newspaper Arctic Springtail Natural Environment Research Council: NERC Open Research Archive Arctic Archives of Insect Biochemistry and Physiology 82 2 59 70 |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
unknown |
description |
The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 °C (control) versus −2 °C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 °C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 °C versus 5 °C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 °C (controls) versus −2 °C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 °C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis. |
format |
Article in Journal/Newspaper |
author |
Grubor-Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. |
spellingShingle |
Grubor-Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola) |
author_facet |
Grubor-Lajšić, Gordana Petri, Edward T. Kojić, Danijela Purać, Jelena Popović, Željko D. Worland, Roger M. Clark, Melody S. Mojović, Miloš Blagojević, Duško P. |
author_sort |
Grubor-Lajšić, Gordana |
title |
Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola) |
title_short |
Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola) |
title_full |
Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola) |
title_fullStr |
Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola) |
title_full_unstemmed |
Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola) |
title_sort |
hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of megaphorura arctica(onychiuridae: collembola) |
publisher |
Wiley |
publishDate |
2013 |
url |
http://nora.nerc.ac.uk/id/eprint/500338/ https://doi.org/10.1002/arch.21073 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Springtail |
genre_facet |
Arctic Springtail |
op_relation |
Grubor-Lajšić, Gordana; Petri, Edward T.; Kojić, Danijela; Purać, Jelena; Popović, Željko D.; Worland, Roger M.; Clark, Melody S. orcid:0000-0002-3442-3824 Mojović, Miloš; Blagojević, Duško P. 2013 Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica(Onychiuridae: Collembola). Archives of Insect Biochemistry and Physiology, 82 (2). 59-70. https://doi.org/10.1002/arch.21073 <https://doi.org/10.1002/arch.21073> |
op_doi |
https://doi.org/10.1002/arch.21073 |
container_title |
Archives of Insect Biochemistry and Physiology |
container_volume |
82 |
container_issue |
2 |
container_start_page |
59 |
op_container_end_page |
70 |
_version_ |
1790596520253325312 |