Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray.
The physiology of the Antarctic microarthropod, Cryptopygus antarcticus, has been well studied, particularly with regard to its ability to withstand low winter temperatures. However, the molecular mechanisms underlying this phenomenon are still poorly understood. 1180 sequences (Expressed Sequence T...
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ftnerc:oai:nora.nerc.ac.uk:4231 2024-06-09T07:39:24+00:00 Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. Purac, Jelena Burns, Gavin Thorne, Michael A.S. Grubor-Lajsic, Gordana Worland, M. Roger Clark, Melody S. 2008 text http://nora.nerc.ac.uk/id/eprint/4231/ https://nora.nerc.ac.uk/id/eprint/4231/1/array.pdf https://doi.org/10.1016/j.jinsphys.2008.07.012 en eng Elsevier https://nora.nerc.ac.uk/id/eprint/4231/1/array.pdf Purac, Jelena; Burns, Gavin; Thorne, Michael A.S. orcid:0000-0001-7759-612X Grubor-Lajsic, Gordana; Worland, M. Roger; Clark, Melody S. orcid:0000-0002-3442-3824 . 2008 Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. Journal of Insect Physiology, 54 (9). 1356-1362. https://doi.org/10.1016/j.jinsphys.2008.07.012 <https://doi.org/10.1016/j.jinsphys.2008.07.012> Zoology Biology and Microbiology Publication - Article PeerReviewed 2008 ftnerc https://doi.org/10.1016/j.jinsphys.2008.07.012 2024-05-15T08:46:53Z The physiology of the Antarctic microarthropod, Cryptopygus antarcticus, has been well studied, particularly with regard to its ability to withstand low winter temperatures. However, the molecular mechanisms underlying this phenomenon are still poorly understood. 1180 sequences (Expressed Sequence Tags or ESTs) were generated and analysed, from populations of C. antarcticus. This represents the first publicly available sequence data for this species. A sub-set (672 clones) were used to generate a small microarray to examine the differences in gene expression between summer acclimated cold tolerant and non-cold tolerant springtails. Although 60% of the clones showed no sequence similarity to annotated genes in the datasets, of those where putative function could be inferred via database homology, there was a clear pattern of up-regulation of structural proteins being associated with the cold tolerant group. These structural proteins mainly comprised cuticle proteins and provide support for the recent theory that summer SCP variation within Collembola species could be a consequence of moulting, with moulting population having lowered SCPs Article in Journal/Newspaper Antarc* Antarctic Antarctic Springtail antarcticus Cryptopygus antarcticus Springtail Natural Environment Research Council: NERC Open Research Archive Antarctic The Antarctic Journal of Insect Physiology 54 9 1356 1362 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
topic |
Zoology Biology and Microbiology |
spellingShingle |
Zoology Biology and Microbiology Purac, Jelena Burns, Gavin Thorne, Michael A.S. Grubor-Lajsic, Gordana Worland, M. Roger Clark, Melody S. Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. |
topic_facet |
Zoology Biology and Microbiology |
description |
The physiology of the Antarctic microarthropod, Cryptopygus antarcticus, has been well studied, particularly with regard to its ability to withstand low winter temperatures. However, the molecular mechanisms underlying this phenomenon are still poorly understood. 1180 sequences (Expressed Sequence Tags or ESTs) were generated and analysed, from populations of C. antarcticus. This represents the first publicly available sequence data for this species. A sub-set (672 clones) were used to generate a small microarray to examine the differences in gene expression between summer acclimated cold tolerant and non-cold tolerant springtails. Although 60% of the clones showed no sequence similarity to annotated genes in the datasets, of those where putative function could be inferred via database homology, there was a clear pattern of up-regulation of structural proteins being associated with the cold tolerant group. These structural proteins mainly comprised cuticle proteins and provide support for the recent theory that summer SCP variation within Collembola species could be a consequence of moulting, with moulting population having lowered SCPs |
format |
Article in Journal/Newspaper |
author |
Purac, Jelena Burns, Gavin Thorne, Michael A.S. Grubor-Lajsic, Gordana Worland, M. Roger Clark, Melody S. |
author_facet |
Purac, Jelena Burns, Gavin Thorne, Michael A.S. Grubor-Lajsic, Gordana Worland, M. Roger Clark, Melody S. |
author_sort |
Purac, Jelena |
title |
Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. |
title_short |
Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. |
title_full |
Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. |
title_fullStr |
Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. |
title_full_unstemmed |
Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. |
title_sort |
cold hardening processes in the antarctic springtail, cryptopygus antarcticus: clues from a microarray. |
publisher |
Elsevier |
publishDate |
2008 |
url |
http://nora.nerc.ac.uk/id/eprint/4231/ https://nora.nerc.ac.uk/id/eprint/4231/1/array.pdf https://doi.org/10.1016/j.jinsphys.2008.07.012 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Springtail antarcticus Cryptopygus antarcticus Springtail |
genre_facet |
Antarc* Antarctic Antarctic Springtail antarcticus Cryptopygus antarcticus Springtail |
op_relation |
https://nora.nerc.ac.uk/id/eprint/4231/1/array.pdf Purac, Jelena; Burns, Gavin; Thorne, Michael A.S. orcid:0000-0001-7759-612X Grubor-Lajsic, Gordana; Worland, M. Roger; Clark, Melody S. orcid:0000-0002-3442-3824 . 2008 Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: clues from a microarray. Journal of Insect Physiology, 54 (9). 1356-1362. https://doi.org/10.1016/j.jinsphys.2008.07.012 <https://doi.org/10.1016/j.jinsphys.2008.07.012> |
op_doi |
https://doi.org/10.1016/j.jinsphys.2008.07.012 |
container_title |
Journal of Insect Physiology |
container_volume |
54 |
container_issue |
9 |
container_start_page |
1356 |
op_container_end_page |
1362 |
_version_ |
1801379303295811584 |