Characterisation of the warm acclimated protein gene (wap65) in the Antarctic plunderfish (Harpagifer antarcticus)

• Published in: DNA Sequence
• Main Authors: Clark, Melody S., Burns, Gavin
• Format: Article in Journal/Newspaper
• Language: English
• Published: Informa Healthcare 2008
• Subjects: Zoology; Biology and Microbiology; Antarctic; The Antarctic; Antarc*; antarcticus
• Online Access: http://nora.nerc.ac.uk/id/eprint/3956/; https://nora.nerc.ac.uk/id/eprint/3956/1/Wap_65.pdf; https://doi.org/10.1080/10425170701388586

Physiological adaptation to increased environmental temperatures has been studied experimentally in a number of fish species, with the up-regulation of several genes identified as being associated with the process, such as the warm-acclimated protein (wap-65). This article describes the cloning and characterisation of the wap65-2 gene from the Antarctic plunderfish (Harpagifer antarcticus). The transcriptional expression of this gene in response to elevated seawater temperatures over a time course series is presented. Initially there is strong down-regulation of this gene to a maximum of 40-fold within 4 h, followed by recovery to almost control levels within 48 h, indicating that this gene does not play a role in the potential temperature adaptation of H. antarcticus.