Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds
This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, S...
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Online Access: | http://nora.nerc.ac.uk/id/eprint/1893/ https://doi.org/10.1016/j.syapm.2005.03.002 |
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ftnerc:oai:nora.nerc.ac.uk:1893 2024-06-09T07:41:10+00:00 Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds Moosvi, S. Azra McDonald, Ian R. Pearce, David A. Kelly, Donovan P. Wood, Ann P. 2005 http://nora.nerc.ac.uk/id/eprint/1893/ https://doi.org/10.1016/j.syapm.2005.03.002 unknown Elsevier Moosvi, S. Azra; McDonald, Ian R.; Pearce, David A. orcid:0000-0001-5292-4596 Kelly, Donovan P.; Wood, Ann P. 2005 Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds. Systematic and Applied Microbiology, 28 (6). 541-554. https://doi.org/10.1016/j.syapm.2005.03.002 <https://doi.org/10.1016/j.syapm.2005.03.002> Biology and Microbiology Publication - Article PeerReviewed 2005 ftnerc https://doi.org/10.1016/j.syapm.2005.03.002 2024-05-15T08:42:16Z This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, South Orkney Islands, Antarctica. Genes encoding the mxaF of methanol dehydrogenase, the fdxA for Afipia ferredoxin, the msmA of methanesulfonate monooxygenase, and the 16S rRNA gene of Methylobacterium were detected in all samples tested. The mxaF gene sequences corresponded to those of Hyphomicrobium, Methylobacterium, and Methylomonas. Over 30 pure cultures of methylotrophs were isolated on methanesulfonate, dimethylsulfone, or dimethylsulfide from ten Signy Island lakes. Some were identified from 16S rRNA gene sequences (and morphology) as Hyphomicrobium species, strains of Afipia felis, and a methylotrophic Flavobacterium strain. Antarctic environments thus contain diverse methylotrophic bacteria, growing on various C1-substrates, including C1-sulfur compounds. Article in Journal/Newspaper Antarc* Antarctic Antarctica Signy Island South Orkney Islands Natural Environment Research Council: NERC Open Research Archive Antarctic Island Lakes ENVELOPE(-128.226,-128.226,62.344,62.344) Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Systematic and Applied Microbiology 28 6 541 554 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
unknown |
topic |
Biology and Microbiology |
spellingShingle |
Biology and Microbiology Moosvi, S. Azra McDonald, Ian R. Pearce, David A. Kelly, Donovan P. Wood, Ann P. Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
topic_facet |
Biology and Microbiology |
description |
This study is the first demonstration that a diverse facultatively methylotrophic microbiota exists in some Antarctic locations. PCR amplification of genes diagnostic for methylotrophs was carried out with bacterial DNA isolated from 14 soil and sediment samples from ten locations on Signy Island, South Orkney Islands, Antarctica. Genes encoding the mxaF of methanol dehydrogenase, the fdxA for Afipia ferredoxin, the msmA of methanesulfonate monooxygenase, and the 16S rRNA gene of Methylobacterium were detected in all samples tested. The mxaF gene sequences corresponded to those of Hyphomicrobium, Methylobacterium, and Methylomonas. Over 30 pure cultures of methylotrophs were isolated on methanesulfonate, dimethylsulfone, or dimethylsulfide from ten Signy Island lakes. Some were identified from 16S rRNA gene sequences (and morphology) as Hyphomicrobium species, strains of Afipia felis, and a methylotrophic Flavobacterium strain. Antarctic environments thus contain diverse methylotrophic bacteria, growing on various C1-substrates, including C1-sulfur compounds. |
format |
Article in Journal/Newspaper |
author |
Moosvi, S. Azra McDonald, Ian R. Pearce, David A. Kelly, Donovan P. Wood, Ann P. |
author_facet |
Moosvi, S. Azra McDonald, Ian R. Pearce, David A. Kelly, Donovan P. Wood, Ann P. |
author_sort |
Moosvi, S. Azra |
title |
Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_short |
Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_full |
Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_fullStr |
Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_full_unstemmed |
Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
title_sort |
molecular detection and isolation from antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds |
publisher |
Elsevier |
publishDate |
2005 |
url |
http://nora.nerc.ac.uk/id/eprint/1893/ https://doi.org/10.1016/j.syapm.2005.03.002 |
long_lat |
ENVELOPE(-128.226,-128.226,62.344,62.344) ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(-45.500,-45.500,-60.583,-60.583) |
geographic |
Antarctic Island Lakes Signy Island South Orkney Islands |
geographic_facet |
Antarctic Island Lakes Signy Island South Orkney Islands |
genre |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
op_relation |
Moosvi, S. Azra; McDonald, Ian R.; Pearce, David A. orcid:0000-0001-5292-4596 Kelly, Donovan P.; Wood, Ann P. 2005 Molecular detection and isolation from Antarctica of methylotrophic bacteria able to grow with methylated sulfur compounds. Systematic and Applied Microbiology, 28 (6). 541-554. https://doi.org/10.1016/j.syapm.2005.03.002 <https://doi.org/10.1016/j.syapm.2005.03.002> |
op_doi |
https://doi.org/10.1016/j.syapm.2005.03.002 |
container_title |
Systematic and Applied Microbiology |
container_volume |
28 |
container_issue |
6 |
container_start_page |
541 |
op_container_end_page |
554 |
_version_ |
1801369600137363456 |