Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens
Lichens in Antarctic habitats are subjected to environmental extremes, including UVB radiation, desiccation and low temperatures, as well as to rapid fluctuations in these. Lichens synthesise a variety of chemical compounds in response to their environmental conditions which contribute towards their...
Published in: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
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Online Access: | http://nora.nerc.ac.uk/id/eprint/17590/ http://www.sciencedirect.com/science/article/pii/S1386142503000738 |
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ftnerc:oai:nora.nerc.ac.uk:17590 2023-05-15T13:45:12+02:00 Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens Edwards, H.G.M. Newton, E.M. Wynn-Williams, D.D. Smith, R.I.L. 2003 http://nora.nerc.ac.uk/id/eprint/17590/ http://www.sciencedirect.com/science/article/pii/S1386142503000738 unknown Elsevier Edwards, H.G.M.; Newton, E.M.; Wynn-Williams, D.D.; Smith, R.I.L. 2003 Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens. Spectrochimica Acta Part A, 59 (10). 2301-2309. https://doi.org/10.1016/S1386-1425(03)00073-8 <https://doi.org/10.1016/S1386-1425(03)00073-8> Botany Publication - Article PeerReviewed 2003 ftnerc https://doi.org/10.1016/S1386-1425(03)00073-8 2023-02-04T19:31:13Z Lichens in Antarctic habitats are subjected to environmental extremes, including UVB radiation, desiccation and low temperatures, as well as to rapid fluctuations in these. Lichens synthesise a variety of chemical compounds in response to their environmental conditions which contribute towards their colour, and which act as protectants against physiological stresses. The fluorescence generated by the lichens at 532 nm can be used in epifluorescence microscopy to identify their presence on substrata but this can severely affect the Raman spectra using visible excitation. The advantage of the near infrared excitation used in FT-Raman spectroscopy in minimising fluorescence emission facilitates the molecular characterisation of lichen encrustations without having to remove the thallus from its substrate or remove or otherwise damage any part of the thallus. Spectroscopic biomarkers are proposed which allow the lichens to be characterised by the identification of characteristic lichen substances; the use of these biomarkers for the preliminary taxonomic identification of Antarctic lichens is examined and some potential pitfalls are described. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 59 10 2301 2309 |
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Natural Environment Research Council: NERC Open Research Archive |
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ftnerc |
language |
unknown |
topic |
Botany |
spellingShingle |
Botany Edwards, H.G.M. Newton, E.M. Wynn-Williams, D.D. Smith, R.I.L. Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens |
topic_facet |
Botany |
description |
Lichens in Antarctic habitats are subjected to environmental extremes, including UVB radiation, desiccation and low temperatures, as well as to rapid fluctuations in these. Lichens synthesise a variety of chemical compounds in response to their environmental conditions which contribute towards their colour, and which act as protectants against physiological stresses. The fluorescence generated by the lichens at 532 nm can be used in epifluorescence microscopy to identify their presence on substrata but this can severely affect the Raman spectra using visible excitation. The advantage of the near infrared excitation used in FT-Raman spectroscopy in minimising fluorescence emission facilitates the molecular characterisation of lichen encrustations without having to remove the thallus from its substrate or remove or otherwise damage any part of the thallus. Spectroscopic biomarkers are proposed which allow the lichens to be characterised by the identification of characteristic lichen substances; the use of these biomarkers for the preliminary taxonomic identification of Antarctic lichens is examined and some potential pitfalls are described. |
format |
Article in Journal/Newspaper |
author |
Edwards, H.G.M. Newton, E.M. Wynn-Williams, D.D. Smith, R.I.L. |
author_facet |
Edwards, H.G.M. Newton, E.M. Wynn-Williams, D.D. Smith, R.I.L. |
author_sort |
Edwards, H.G.M. |
title |
Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens |
title_short |
Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens |
title_full |
Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens |
title_fullStr |
Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens |
title_full_unstemmed |
Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens |
title_sort |
non-destructive analysis of pigments and other organic compounds in lichens using fourier-transform raman spectroscopy: a study of antarctic epilithic lichens |
publisher |
Elsevier |
publishDate |
2003 |
url |
http://nora.nerc.ac.uk/id/eprint/17590/ http://www.sciencedirect.com/science/article/pii/S1386142503000738 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Edwards, H.G.M.; Newton, E.M.; Wynn-Williams, D.D.; Smith, R.I.L. 2003 Non-destructive analysis of pigments and other organic compounds in lichens using Fourier-transform Raman spectroscopy: a study of Antarctic epilithic lichens. Spectrochimica Acta Part A, 59 (10). 2301-2309. https://doi.org/10.1016/S1386-1425(03)00073-8 <https://doi.org/10.1016/S1386-1425(03)00073-8> |
op_doi |
https://doi.org/10.1016/S1386-1425(03)00073-8 |
container_title |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
container_volume |
59 |
container_issue |
10 |
container_start_page |
2301 |
op_container_end_page |
2309 |
_version_ |
1766216402285363200 |