Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba)
Background: All crustaceans periodically moult to renew their exoskeleton. In krill this involves partial digestion and resorption of the old exoskeleton and synthesis of new cuticle. Molecular events that underlie the moult cycle are poorly understood in calcifying crustaceans and even less so in n...
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Biomed Central Ltd
2010
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ftnerc:oai:nora.nerc.ac.uk:14832 2023-05-15T13:45:11+02:00 Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) Seear, Paul J. Tarling, Geraint A. Burns, Gavin Goodall-Copestake, Will P. Gaten, Edward Ozkaya, Ozge Rosato, Ezio 2010 text http://nora.nerc.ac.uk/id/eprint/14832/ https://nora.nerc.ac.uk/id/eprint/14832/1/1471-2164-11-582.pdf http://www.biomedcentral.com/1471-2164/11/582 en eng Biomed Central Ltd https://nora.nerc.ac.uk/id/eprint/14832/1/1471-2164-11-582.pdf Seear, Paul J.; Tarling, Geraint A. orcid:0000-0002-3753-5899 Burns, Gavin; Goodall-Copestake, Will P. orcid:0000-0003-3586-9091 Gaten, Edward; Ozkaya, Ozge; Rosato, Ezio. 2010 Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba). BMC Genomics, 11, 582. 13, pp. https://doi.org/10.1186/1471-2164-11-582 <https://doi.org/10.1186/1471-2164-11-582> cc_by CC-BY Marine Sciences Biology and Microbiology Publication - Article PeerReviewed 2010 ftnerc https://doi.org/10.1186/1471-2164-11-582 2023-02-04T19:29:27Z Background: All crustaceans periodically moult to renew their exoskeleton. In krill this involves partial digestion and resorption of the old exoskeleton and synthesis of new cuticle. Molecular events that underlie the moult cycle are poorly understood in calcifying crustaceans and even less so in non-calcifying organisms such as krill. To address this we constructed an Antarctic krill cDNA microarray in order to generate gene expression profiles across the moult cycle and identify possible activation pathways. Results: A total of 26 different cuticle genes were identified that showed differential gene expression across the moult cycle. Almost all cuticle genes were up regulated during premoult and down regulated during late intermoult. There were a number of transcripts with significant sequence homology to genes potentially involved in the synthesis, breakdown and resorption of chitin. During early premoult glutamine synthetase, a gene involved in generating an amino acid used in the synthesis of glucosamine, a constituent of chitin, was up regulated more than twofold. Mannosyltransferase 1, a member of the glycosyltransferase family of enzymes that includes chitin synthase was also up regulated during early premoult. Transcripts homologous to a beta-N-acetylglucosaminidase (beta-NAGase) precursor were expressed at a higher level during late intermoult (prior to apolysis) than during premoult. This observation coincided with the up regulation during late intermoult, of a coatomer subunit epsilon involved in the production of vesicles that maybe used to transport the beta-NAGase precursors into the exuvial cleft. Trypsin, known to activate the beta-NAGase precursor, was up regulated more than fourfold during premoult. The up regulation of a predicted oligopeptide transporter during premoult may allow the transport of chitin breakdown products across the newly synthesised epi- and exocuticle layers. Conclusion: We have identified many genes differentially expressed across the moult cycle of krill that correspond ... Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Natural Environment Research Council: NERC Open Research Archive Antarctic BMC Genomics 11 1 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
English |
topic |
Marine Sciences Biology and Microbiology |
spellingShingle |
Marine Sciences Biology and Microbiology Seear, Paul J. Tarling, Geraint A. Burns, Gavin Goodall-Copestake, Will P. Gaten, Edward Ozkaya, Ozge Rosato, Ezio Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) |
topic_facet |
Marine Sciences Biology and Microbiology |
description |
Background: All crustaceans periodically moult to renew their exoskeleton. In krill this involves partial digestion and resorption of the old exoskeleton and synthesis of new cuticle. Molecular events that underlie the moult cycle are poorly understood in calcifying crustaceans and even less so in non-calcifying organisms such as krill. To address this we constructed an Antarctic krill cDNA microarray in order to generate gene expression profiles across the moult cycle and identify possible activation pathways. Results: A total of 26 different cuticle genes were identified that showed differential gene expression across the moult cycle. Almost all cuticle genes were up regulated during premoult and down regulated during late intermoult. There were a number of transcripts with significant sequence homology to genes potentially involved in the synthesis, breakdown and resorption of chitin. During early premoult glutamine synthetase, a gene involved in generating an amino acid used in the synthesis of glucosamine, a constituent of chitin, was up regulated more than twofold. Mannosyltransferase 1, a member of the glycosyltransferase family of enzymes that includes chitin synthase was also up regulated during early premoult. Transcripts homologous to a beta-N-acetylglucosaminidase (beta-NAGase) precursor were expressed at a higher level during late intermoult (prior to apolysis) than during premoult. This observation coincided with the up regulation during late intermoult, of a coatomer subunit epsilon involved in the production of vesicles that maybe used to transport the beta-NAGase precursors into the exuvial cleft. Trypsin, known to activate the beta-NAGase precursor, was up regulated more than fourfold during premoult. The up regulation of a predicted oligopeptide transporter during premoult may allow the transport of chitin breakdown products across the newly synthesised epi- and exocuticle layers. Conclusion: We have identified many genes differentially expressed across the moult cycle of krill that correspond ... |
format |
Article in Journal/Newspaper |
author |
Seear, Paul J. Tarling, Geraint A. Burns, Gavin Goodall-Copestake, Will P. Gaten, Edward Ozkaya, Ozge Rosato, Ezio |
author_facet |
Seear, Paul J. Tarling, Geraint A. Burns, Gavin Goodall-Copestake, Will P. Gaten, Edward Ozkaya, Ozge Rosato, Ezio |
author_sort |
Seear, Paul J. |
title |
Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) |
title_short |
Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) |
title_full |
Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) |
title_fullStr |
Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) |
title_full_unstemmed |
Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) |
title_sort |
differential gene expression during the moult cycle of antarctic krill (euphausia superba) |
publisher |
Biomed Central Ltd |
publishDate |
2010 |
url |
http://nora.nerc.ac.uk/id/eprint/14832/ https://nora.nerc.ac.uk/id/eprint/14832/1/1471-2164-11-582.pdf http://www.biomedcentral.com/1471-2164/11/582 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba |
op_relation |
https://nora.nerc.ac.uk/id/eprint/14832/1/1471-2164-11-582.pdf Seear, Paul J.; Tarling, Geraint A. orcid:0000-0002-3753-5899 Burns, Gavin; Goodall-Copestake, Will P. orcid:0000-0003-3586-9091 Gaten, Edward; Ozkaya, Ozge; Rosato, Ezio. 2010 Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba). BMC Genomics, 11, 582. 13, pp. https://doi.org/10.1186/1471-2164-11-582 <https://doi.org/10.1186/1471-2164-11-582> |
op_rights |
cc_by |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/1471-2164-11-582 |
container_title |
BMC Genomics |
container_volume |
11 |
container_issue |
1 |
_version_ |
1766215769470795776 |