Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques
The biodiversity of the pelagic bacterioplankton community of a maritime Antarctic freshwater lake was examined by cultivation-dependent and cultivation-independent techniques to determine predominant bacterioplankton populations present. The culture-dependent techniques used were direct culture and...
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2003
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ftnerc:oai:nora.nerc.ac.uk:12842 2023-05-15T13:45:11+02:00 Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques Pearce, D.A. van der Gast, C.J. Lawley, B. Ellis-Evans, J.C. 2003 http://nora.nerc.ac.uk/id/eprint/12842/ http://onlinelibrary.wiley.com/doi/10.1016/S0168-6496%2803%2900110-7/full unknown Elsevier Pearce, D.A. orcid:0000-0001-5292-4596 van der Gast, C.J.; Lawley, B.; Ellis-Evans, J.C. 2003 Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques. FEMS Microbiology Ecology, 45 (1). 59-70. https://doi.org/10.1016/S0168-6496(03)00110-7 <https://doi.org/10.1016/S0168-6496(03)00110-7> Biology and Microbiology Ecology and Environment Publication - Article PeerReviewed 2003 ftnerc https://doi.org/10.1016/S0168-6496(03)00110-7 2023-02-04T19:28:13Z The biodiversity of the pelagic bacterioplankton community of a maritime Antarctic freshwater lake was examined by cultivation-dependent and cultivation-independent techniques to determine predominant bacterioplankton populations present. The culture-dependent techniques used were direct culture and observation, polymerase chain reaction amplification of 16S rRNA gene fragments, restriction fragment length polymorphism (RFLP) analysis followed by selective sequencing and fatty acid methyl ester analysis. The culture-independent techniques used were 16S ribosomal DNA gene cloning, RFLP analysis and sequencing, in situ hybridisation with group-specific, fluorescently labelled oligonucleotide probes and cloning and sequencing of dominant denaturing gradient gel electrophoresis products. Significant differences occurred between the results obtained with each method. However, sufficient overlap existed between the different methods to identify potentially significant groups. At least six different bacterial divisions including 24 genera were identified using culture-dependent techniques, and eight different bacterial divisions, including 23 genera, were identified using culture-independent techniques. Only five genera, Corynebacterium, Cytophaga, Flavobacterium, Janthinobacterium and Pseudomonas, could be identified using both sets of techniques, which represented four different bacterial divisions. Significantly for Antarctic freshwater lakes, pigment production is found within members of each of these genera. This work illustrates the importance of a comprehensive polyphasic approach in the analysis of lake bacterioplankton, and supports the ecological relevance of results obtained in earlier entirely culture-based studies. (C) 2003 Federation of European Microbiological Societies. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic FEMS Microbiology Ecology 45 1 59 70 |
institution |
Open Polar |
collection |
Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
unknown |
topic |
Biology and Microbiology Ecology and Environment |
spellingShingle |
Biology and Microbiology Ecology and Environment Pearce, D.A. van der Gast, C.J. Lawley, B. Ellis-Evans, J.C. Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques |
topic_facet |
Biology and Microbiology Ecology and Environment |
description |
The biodiversity of the pelagic bacterioplankton community of a maritime Antarctic freshwater lake was examined by cultivation-dependent and cultivation-independent techniques to determine predominant bacterioplankton populations present. The culture-dependent techniques used were direct culture and observation, polymerase chain reaction amplification of 16S rRNA gene fragments, restriction fragment length polymorphism (RFLP) analysis followed by selective sequencing and fatty acid methyl ester analysis. The culture-independent techniques used were 16S ribosomal DNA gene cloning, RFLP analysis and sequencing, in situ hybridisation with group-specific, fluorescently labelled oligonucleotide probes and cloning and sequencing of dominant denaturing gradient gel electrophoresis products. Significant differences occurred between the results obtained with each method. However, sufficient overlap existed between the different methods to identify potentially significant groups. At least six different bacterial divisions including 24 genera were identified using culture-dependent techniques, and eight different bacterial divisions, including 23 genera, were identified using culture-independent techniques. Only five genera, Corynebacterium, Cytophaga, Flavobacterium, Janthinobacterium and Pseudomonas, could be identified using both sets of techniques, which represented four different bacterial divisions. Significantly for Antarctic freshwater lakes, pigment production is found within members of each of these genera. This work illustrates the importance of a comprehensive polyphasic approach in the analysis of lake bacterioplankton, and supports the ecological relevance of results obtained in earlier entirely culture-based studies. (C) 2003 Federation of European Microbiological Societies. |
format |
Article in Journal/Newspaper |
author |
Pearce, D.A. van der Gast, C.J. Lawley, B. Ellis-Evans, J.C. |
author_facet |
Pearce, D.A. van der Gast, C.J. Lawley, B. Ellis-Evans, J.C. |
author_sort |
Pearce, D.A. |
title |
Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques |
title_short |
Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques |
title_full |
Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques |
title_fullStr |
Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques |
title_full_unstemmed |
Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques |
title_sort |
bacterioplankton community diversity in a maritime antarctic lake, determined by culture-dependent and culture-independent techniques |
publisher |
Elsevier |
publishDate |
2003 |
url |
http://nora.nerc.ac.uk/id/eprint/12842/ http://onlinelibrary.wiley.com/doi/10.1016/S0168-6496%2803%2900110-7/full |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Pearce, D.A. orcid:0000-0001-5292-4596 van der Gast, C.J.; Lawley, B.; Ellis-Evans, J.C. 2003 Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques. FEMS Microbiology Ecology, 45 (1). 59-70. https://doi.org/10.1016/S0168-6496(03)00110-7 <https://doi.org/10.1016/S0168-6496(03)00110-7> |
op_doi |
https://doi.org/10.1016/S0168-6496(03)00110-7 |
container_title |
FEMS Microbiology Ecology |
container_volume |
45 |
container_issue |
1 |
container_start_page |
59 |
op_container_end_page |
70 |
_version_ |
1766215277188481024 |