Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities
Aims: To exploit the cyanobacterial diversity of microbial mats growing in the benthic environment of Antarctic lakes for the discovery of novel antibiotic and antitumour activities. Methods and results: In all, 51 Antarctic cyanobacteria isolated from benthic mats were cultivated in the laboratory...
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2008
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ftnerc:oai:nora.nerc.ac.uk:11399 2023-05-15T13:45:10+02:00 Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities Biondi, N. Tredici, M.R. Taton, A. Wilmotte, A. Hodgson, Dominic A. Losi, D. Marinelli, F. 2008 http://nora.nerc.ac.uk/id/eprint/11399/ unknown Blackwell Biondi, N.; Tredici, M.R.; Taton, A.; Wilmotte, A.; Hodgson, Dominic A. orcid:0000-0002-3841-3746 Losi, D.; Marinelli, F. 2008 Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities. Journal of Applied Microbiology, 105 (1). 105-115. https://doi.org/10.1111/j.1365-2672.2007.03716.x <https://doi.org/10.1111/j.1365-2672.2007.03716.x> Biology and Microbiology Health Publication - Article PeerReviewed 2008 ftnerc https://doi.org/10.1111/j.1365-2672.2007.03716.x 2023-02-04T19:27:21Z Aims: To exploit the cyanobacterial diversity of microbial mats growing in the benthic environment of Antarctic lakes for the discovery of novel antibiotic and antitumour activities. Methods and results: In all, 51 Antarctic cyanobacteria isolated from benthic mats were cultivated in the laboratory by optimizing temperature, irradiance and mixing. Productivity was generally very low (<= 60 mg l(-1) d(-1)) with growth rates (mu) in the range of 0.02-0.44 d(-1). Growth rates were limited by photosensitivity, sensitivity to air bubbling, polysaccharide production or cell aggregation. Despite this, 126 extracts were prepared from 48 strains and screened for antimicrobial and cytotoxic activities. Seventeen cyanobacteria showed antimicrobial activity (against the Gram-positive Staphylococcus aureus, the filamentous fungus Aspergillus fumigatus or the yeast Cryptococcus neoformans), and 25 were cytotoxic. The bioactivities were not in accordance with the phylogenetic grouping, but rather strain-specific. One active strain was cultivated in a 10-l photobioreactor. Conclusions: Isolation and mass cultivation of Antarctic cyanobacteria and LC-MS (liquid chromatography/mass spectrometry) fractionation of extracts from a subset of those strains (hits) that exhibited relatively potent antibacterial and/or antifungal activities, evidenced a chemical novelty worthy of further investigation. Significance and impact of the study: Development of isolation, cultivation and screening methods for Antarctic cyanobacteria has led to the discovery of strains endowed with interesting antimicrobial and antitumour activities. Article in Journal/Newspaper Antarc* Antarctic Natural Environment Research Council: NERC Open Research Archive Antarctic Journal of Applied Microbiology 105 1 105 115 |
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Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
unknown |
topic |
Biology and Microbiology Health |
spellingShingle |
Biology and Microbiology Health Biondi, N. Tredici, M.R. Taton, A. Wilmotte, A. Hodgson, Dominic A. Losi, D. Marinelli, F. Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities |
topic_facet |
Biology and Microbiology Health |
description |
Aims: To exploit the cyanobacterial diversity of microbial mats growing in the benthic environment of Antarctic lakes for the discovery of novel antibiotic and antitumour activities. Methods and results: In all, 51 Antarctic cyanobacteria isolated from benthic mats were cultivated in the laboratory by optimizing temperature, irradiance and mixing. Productivity was generally very low (<= 60 mg l(-1) d(-1)) with growth rates (mu) in the range of 0.02-0.44 d(-1). Growth rates were limited by photosensitivity, sensitivity to air bubbling, polysaccharide production or cell aggregation. Despite this, 126 extracts were prepared from 48 strains and screened for antimicrobial and cytotoxic activities. Seventeen cyanobacteria showed antimicrobial activity (against the Gram-positive Staphylococcus aureus, the filamentous fungus Aspergillus fumigatus or the yeast Cryptococcus neoformans), and 25 were cytotoxic. The bioactivities were not in accordance with the phylogenetic grouping, but rather strain-specific. One active strain was cultivated in a 10-l photobioreactor. Conclusions: Isolation and mass cultivation of Antarctic cyanobacteria and LC-MS (liquid chromatography/mass spectrometry) fractionation of extracts from a subset of those strains (hits) that exhibited relatively potent antibacterial and/or antifungal activities, evidenced a chemical novelty worthy of further investigation. Significance and impact of the study: Development of isolation, cultivation and screening methods for Antarctic cyanobacteria has led to the discovery of strains endowed with interesting antimicrobial and antitumour activities. |
format |
Article in Journal/Newspaper |
author |
Biondi, N. Tredici, M.R. Taton, A. Wilmotte, A. Hodgson, Dominic A. Losi, D. Marinelli, F. |
author_facet |
Biondi, N. Tredici, M.R. Taton, A. Wilmotte, A. Hodgson, Dominic A. Losi, D. Marinelli, F. |
author_sort |
Biondi, N. |
title |
Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities |
title_short |
Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities |
title_full |
Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities |
title_fullStr |
Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities |
title_full_unstemmed |
Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities |
title_sort |
cyanobacteria from benthic mats of antarctic lakes as a source of new bioactivities |
publisher |
Blackwell |
publishDate |
2008 |
url |
http://nora.nerc.ac.uk/id/eprint/11399/ |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Biondi, N.; Tredici, M.R.; Taton, A.; Wilmotte, A.; Hodgson, Dominic A. orcid:0000-0002-3841-3746 Losi, D.; Marinelli, F. 2008 Cyanobacteria from benthic mats of Antarctic lakes as a source of new bioactivities. Journal of Applied Microbiology, 105 (1). 105-115. https://doi.org/10.1111/j.1365-2672.2007.03716.x <https://doi.org/10.1111/j.1365-2672.2007.03716.x> |
op_doi |
https://doi.org/10.1111/j.1365-2672.2007.03716.x |
container_title |
Journal of Applied Microbiology |
container_volume |
105 |
container_issue |
1 |
container_start_page |
105 |
op_container_end_page |
115 |
_version_ |
1766214542524678144 |