Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic
The leafy liverwort Lophozia excisa, which is colonised by basidiomycete fungi in other biomes and which evidence suggests may be colonised by mycorrhizal fungi in Antarctica, was sampled from L,onie Island in the southern maritime Antarctic (67A degrees 36' S, 68A degrees 21' W). Microsco...
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ftnerc:oai:nora.nerc.ac.uk:10607 2023-05-15T13:45:10+02:00 Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic Newsham, Kevin K. Bridge, Paul D. 2010 http://nora.nerc.ac.uk/id/eprint/10607/ unknown SPRINGER Newsham, Kevin K. orcid:0000-0002-9108-0936 Bridge, Paul D. 2010 Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic. Mycorrhiza, 20 (5). 307-313. https://doi.org/10.1007/s00572-009-0283-9 <https://doi.org/10.1007/s00572-009-0283-9> Biology and Microbiology Ecology and Environment Publication - Article PeerReviewed 2010 ftnerc https://doi.org/10.1007/s00572-009-0283-9 2023-02-04T19:26:49Z The leafy liverwort Lophozia excisa, which is colonised by basidiomycete fungi in other biomes and which evidence suggests may be colonised by mycorrhizal fungi in Antarctica, was sampled from L,onie Island in the southern maritime Antarctic (67A degrees 36' S, 68A degrees 21' W). Microscopic examination of plants indicated that fungal hyphae colonised 78% of the rhizoids of the liverwort, apparently by entering the tips of rhizoids prior to growing into their bases, where they formed hyphal coils. Extensive colonisation of stem medullary cells by hyphae was also observed. DNA was extracted from surface-sterilised liverwort tissues and sequenced following nested PCR, using the primer set ITS1F/TW14, followed by a second round of amplification using the ITSSeb3/TW13 primer set. Neighbour-joining analyses showed that the sequences obtained nested in Sebacinales clade B as a 100% supported sister group to Sebacinales sequences from the leafy liverworts Lophozia sudetica, L. incisa and Calypogeia muelleriana sampled from Europe. Direct PCR using the fungal specific primer set ITS1F/ITS4 similarly identified fungi belonging to Sebacinales clade B as the principal colonists of L. excisa tissues. These observations indicate the presence of a second mycothallus in Antarctica and support the previous suggestion that the Sebacinales has a wide geographical distribution. Article in Journal/Newspaper Antarc* Antarctic Antarctica Natural Environment Research Council: NERC Open Research Archive Antarctic Mycorrhiza 20 5 307 313 |
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Open Polar |
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Natural Environment Research Council: NERC Open Research Archive |
op_collection_id |
ftnerc |
language |
unknown |
topic |
Biology and Microbiology Ecology and Environment |
spellingShingle |
Biology and Microbiology Ecology and Environment Newsham, Kevin K. Bridge, Paul D. Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic |
topic_facet |
Biology and Microbiology Ecology and Environment |
description |
The leafy liverwort Lophozia excisa, which is colonised by basidiomycete fungi in other biomes and which evidence suggests may be colonised by mycorrhizal fungi in Antarctica, was sampled from L,onie Island in the southern maritime Antarctic (67A degrees 36' S, 68A degrees 21' W). Microscopic examination of plants indicated that fungal hyphae colonised 78% of the rhizoids of the liverwort, apparently by entering the tips of rhizoids prior to growing into their bases, where they formed hyphal coils. Extensive colonisation of stem medullary cells by hyphae was also observed. DNA was extracted from surface-sterilised liverwort tissues and sequenced following nested PCR, using the primer set ITS1F/TW14, followed by a second round of amplification using the ITSSeb3/TW13 primer set. Neighbour-joining analyses showed that the sequences obtained nested in Sebacinales clade B as a 100% supported sister group to Sebacinales sequences from the leafy liverworts Lophozia sudetica, L. incisa and Calypogeia muelleriana sampled from Europe. Direct PCR using the fungal specific primer set ITS1F/ITS4 similarly identified fungi belonging to Sebacinales clade B as the principal colonists of L. excisa tissues. These observations indicate the presence of a second mycothallus in Antarctica and support the previous suggestion that the Sebacinales has a wide geographical distribution. |
format |
Article in Journal/Newspaper |
author |
Newsham, Kevin K. Bridge, Paul D. |
author_facet |
Newsham, Kevin K. Bridge, Paul D. |
author_sort |
Newsham, Kevin K. |
title |
Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic |
title_short |
Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic |
title_full |
Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic |
title_fullStr |
Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic |
title_full_unstemmed |
Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic |
title_sort |
sebacinales are associates of the leafy liverwort lophozia excisa in the southern maritime antarctic |
publisher |
SPRINGER |
publishDate |
2010 |
url |
http://nora.nerc.ac.uk/id/eprint/10607/ |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
Newsham, Kevin K. orcid:0000-0002-9108-0936 Bridge, Paul D. 2010 Sebacinales are associates of the leafy liverwort Lophozia excisa in the southern maritime Antarctic. Mycorrhiza, 20 (5). 307-313. https://doi.org/10.1007/s00572-009-0283-9 <https://doi.org/10.1007/s00572-009-0283-9> |
op_doi |
https://doi.org/10.1007/s00572-009-0283-9 |
container_title |
Mycorrhiza |
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20 |
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5 |
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307 |
op_container_end_page |
313 |
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1766214224211607552 |