Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress
Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve t...
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Pascasarjana Universitas Negeri Medan
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Online Access: | https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779 https://doi.org/10.24114/jpkim.v11i3.15779 |
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ftnegerimedanojs:oai:ojs.103.242.233.20:article/15779 2023-05-15T14:55:06+02:00 Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress Silaban, Saronom Simorangkir, Murniaty Gaffar, Shabarni Maksum, Iman Permana Subroto, Toto 2019-12-09 application/pdf https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779 https://doi.org/10.24114/jpkim.v11i3.15779 eng eng Pascasarjana Universitas Negeri Medan https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779/12417 https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779 doi:10.24114/jpkim.v11i3.15779 Copyright (c) 2019 JURNAL PENDIDIKAN KIMIA Jurnal Pendidikan Kimia (JPKim); Vol 11, No 3 (2019): December; 122-128 2549-3116 2085-3653 Kimia Terapan info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article 2019 ftnegerimedanojs https://doi.org/10.24114/jpkim.v11i3.15779 2022-12-27T19:26:23Z Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 was successfully expressed as protein soluble using an optimum temperature of 12°C in E. coli BL21 (DE3) Arctic Express. It was indicated from the 63kDa protein band obtained from the soluble fraction on SDS-PAGE. The higher temperature of fermentation increased the amount of protein in the insoluble fraction due. It concluded that the fermentation temperature affect the rate of prethrombin-2 expression.Keywords: E. coli BL21(DE3) ArcticExpress, prethrombin-2, soluble, temperature Article in Journal/Newspaper Arctic E-Journal Universitas Negeri Medan (Unimed) Arctic Jurnal Pendidikan Kimia 11 3 122 128 |
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Open Polar |
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E-Journal Universitas Negeri Medan (Unimed) |
op_collection_id |
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language |
English |
topic |
Kimia Terapan |
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Kimia Terapan Silaban, Saronom Simorangkir, Murniaty Gaffar, Shabarni Maksum, Iman Permana Subroto, Toto Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress |
topic_facet |
Kimia Terapan |
description |
Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 was successfully expressed as protein soluble using an optimum temperature of 12°C in E. coli BL21 (DE3) Arctic Express. It was indicated from the 63kDa protein band obtained from the soluble fraction on SDS-PAGE. The higher temperature of fermentation increased the amount of protein in the insoluble fraction due. It concluded that the fermentation temperature affect the rate of prethrombin-2 expression.Keywords: E. coli BL21(DE3) ArcticExpress, prethrombin-2, soluble, temperature |
format |
Article in Journal/Newspaper |
author |
Silaban, Saronom Simorangkir, Murniaty Gaffar, Shabarni Maksum, Iman Permana Subroto, Toto |
author_facet |
Silaban, Saronom Simorangkir, Murniaty Gaffar, Shabarni Maksum, Iman Permana Subroto, Toto |
author_sort |
Silaban, Saronom |
title |
Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress |
title_short |
Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress |
title_full |
Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress |
title_fullStr |
Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress |
title_full_unstemmed |
Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress |
title_sort |
temperature effect on expression of recombinant human prethrombin-2 in escherichia coli bl21(de3) arcticexpress |
publisher |
Pascasarjana Universitas Negeri Medan |
publishDate |
2019 |
url |
https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779 https://doi.org/10.24114/jpkim.v11i3.15779 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Jurnal Pendidikan Kimia (JPKim); Vol 11, No 3 (2019): December; 122-128 2549-3116 2085-3653 |
op_relation |
https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779/12417 https://jurnal.unimed.ac.id/2012/index.php/jpk/article/view/15779 doi:10.24114/jpkim.v11i3.15779 |
op_rights |
Copyright (c) 2019 JURNAL PENDIDIKAN KIMIA |
op_doi |
https://doi.org/10.24114/jpkim.v11i3.15779 |
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Jurnal Pendidikan Kimia |
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11 |
container_issue |
3 |
container_start_page |
122 |
op_container_end_page |
128 |
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1766326879180029952 |