Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics
With maritime transport of crude oil from Alaska to California, there is significant potential for a catastrophic spill which could impact migrating salmon. Therefore, this study compared the lethal and sublethal metabolic actions of the water-accommodated fraction (WAF) and the chemically enhanced...
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Online Access: | http://hdl.handle.net/11455/68085 https://doi.org/10.1016/j.aquatox.2009.09.006 |
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ftnchunghsing:oai:ir.lib.nchu.edu.tw:11455/68085 2023-05-15T18:03:38+02:00 Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics Lin, C.Y. Anderson, B.S. Phillips, B.M. Peng, A.C. Clark, S. Voorhees, J. Wu, H.D.I. Martin, M.J. McCall, J. Todd, C.R. Hsieh, F.S. Crane, D. Viant, M.R. Sowby, M.L. Tjeerdema, R.S. 2009 http://hdl.handle.net/11455/68085 https://doi.org/10.1016/j.aquatox.2009.09.006 en_US eng #PLACEHOLDER_PARENT_METADATA_VALUE# Aquatic Toxicology Aquatic Toxicology, Volume 95, Issue 3, Page(s) 230-238. http://dx.doi.org/10.1016/j.aquatox.2009.09.006 0166-445X http://hdl.handle.net/11455/68085 doi:10.1016/j.aquatox.2009.09.006 none NMR Metabolomics Metabonomics Chinook salmon Onchorhyncus tshawytscha Prudhoe Bay crude oil Corexit 9500 Toxicity Oil spill water-accommodated fractions environmental metabolomics h-1-nmr mass-spectrometry aquatic toxicity rat embryogenesis spectroscopy microflora Journal Article 2009 ftnchunghsing https://doi.org/10.1016/j.aquatox.2009.09.006 2021-06-26T20:39:16Z With maritime transport of crude oil from Alaska to California, there is significant potential for a catastrophic spill which could impact migrating salmon. Therefore, this study compared the lethal and sublethal metabolic actions of the water-accommodated fraction (WAF) and the chemically enhanced WAF (CEWAF, via Corexit 9500) of Prudhoe Bay crude oil in smolts of Chinook salmon (Onchorhyncus tshawytscha). After 96-h exposure to the CEWAF, the resulting LC50 was some 20 times higher (i.e., less toxic) than that of the WAR Muscle and liver samples from surviving fish were collected and low-molecular weight metabolites were analyzed using one-dimensional (1)H and projections of two-dimensional (1)H J-resolved NMR. Principal component analysis (PCA), employed to analyze NMR spectra and identify most variance from the samples, revealed age-related metabolic changes in the fish within the replicated studies, but few consistent metabolic effects from the treatments. However, ANOVA results demonstrated that the dose-response metabolite patterns are both metabolite- and organ-dependent. In general, exposure to either WAF or CEWAF resulted in an increase of amino acids (i.e., valine, glutamine and glutamate) and a decrease of both organic osmolytes (i.e., glycerophosphorylcholine) and energetic substrates (i.e., succinate). The simultaneous increase of formate and decrease of glycerophosphorylcholine in the liver, or the decrease of glycerophosphorylcholine in muscle, may serve as sensitive sublethal biomarkers for WAF or CEWAF exposures. respectively. In conclusion, dispersant treatment significantly decreased the lethal potency of crude oil to salmon smolts. and the NMR-based metabolomics approach provided a sensitive means to characterize the sublethal metabolic actions. Published by Elsevier B.V. Article in Journal/Newspaper Prudhoe Bay Alaska National Chung Hsing University Institutional Repository - NCHUIR Aquatic Toxicology 95 3 230 238 |
institution |
Open Polar |
collection |
National Chung Hsing University Institutional Repository - NCHUIR |
op_collection_id |
ftnchunghsing |
language |
English |
topic |
NMR Metabolomics Metabonomics Chinook salmon Onchorhyncus tshawytscha Prudhoe Bay crude oil Corexit 9500 Toxicity Oil spill water-accommodated fractions environmental metabolomics h-1-nmr mass-spectrometry aquatic toxicity rat embryogenesis spectroscopy microflora |
spellingShingle |
NMR Metabolomics Metabonomics Chinook salmon Onchorhyncus tshawytscha Prudhoe Bay crude oil Corexit 9500 Toxicity Oil spill water-accommodated fractions environmental metabolomics h-1-nmr mass-spectrometry aquatic toxicity rat embryogenesis spectroscopy microflora Lin, C.Y. Anderson, B.S. Phillips, B.M. Peng, A.C. Clark, S. Voorhees, J. Wu, H.D.I. Martin, M.J. McCall, J. Todd, C.R. Hsieh, F.S. Crane, D. Viant, M.R. Sowby, M.L. Tjeerdema, R.S. Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics |
topic_facet |
NMR Metabolomics Metabonomics Chinook salmon Onchorhyncus tshawytscha Prudhoe Bay crude oil Corexit 9500 Toxicity Oil spill water-accommodated fractions environmental metabolomics h-1-nmr mass-spectrometry aquatic toxicity rat embryogenesis spectroscopy microflora |
description |
With maritime transport of crude oil from Alaska to California, there is significant potential for a catastrophic spill which could impact migrating salmon. Therefore, this study compared the lethal and sublethal metabolic actions of the water-accommodated fraction (WAF) and the chemically enhanced WAF (CEWAF, via Corexit 9500) of Prudhoe Bay crude oil in smolts of Chinook salmon (Onchorhyncus tshawytscha). After 96-h exposure to the CEWAF, the resulting LC50 was some 20 times higher (i.e., less toxic) than that of the WAR Muscle and liver samples from surviving fish were collected and low-molecular weight metabolites were analyzed using one-dimensional (1)H and projections of two-dimensional (1)H J-resolved NMR. Principal component analysis (PCA), employed to analyze NMR spectra and identify most variance from the samples, revealed age-related metabolic changes in the fish within the replicated studies, but few consistent metabolic effects from the treatments. However, ANOVA results demonstrated that the dose-response metabolite patterns are both metabolite- and organ-dependent. In general, exposure to either WAF or CEWAF resulted in an increase of amino acids (i.e., valine, glutamine and glutamate) and a decrease of both organic osmolytes (i.e., glycerophosphorylcholine) and energetic substrates (i.e., succinate). The simultaneous increase of formate and decrease of glycerophosphorylcholine in the liver, or the decrease of glycerophosphorylcholine in muscle, may serve as sensitive sublethal biomarkers for WAF or CEWAF exposures. respectively. In conclusion, dispersant treatment significantly decreased the lethal potency of crude oil to salmon smolts. and the NMR-based metabolomics approach provided a sensitive means to characterize the sublethal metabolic actions. Published by Elsevier B.V. |
format |
Article in Journal/Newspaper |
author |
Lin, C.Y. Anderson, B.S. Phillips, B.M. Peng, A.C. Clark, S. Voorhees, J. Wu, H.D.I. Martin, M.J. McCall, J. Todd, C.R. Hsieh, F.S. Crane, D. Viant, M.R. Sowby, M.L. Tjeerdema, R.S. |
author_facet |
Lin, C.Y. Anderson, B.S. Phillips, B.M. Peng, A.C. Clark, S. Voorhees, J. Wu, H.D.I. Martin, M.J. McCall, J. Todd, C.R. Hsieh, F.S. Crane, D. Viant, M.R. Sowby, M.L. Tjeerdema, R.S. |
author_sort |
Lin, C.Y. |
title |
Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics |
title_short |
Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics |
title_full |
Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics |
title_fullStr |
Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics |
title_full_unstemmed |
Characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via NMR-based metabolomics |
title_sort |
characterization of the metabolic actions of crude versus dispersed oil in salmon smolts via nmr-based metabolomics |
publishDate |
2009 |
url |
http://hdl.handle.net/11455/68085 https://doi.org/10.1016/j.aquatox.2009.09.006 |
genre |
Prudhoe Bay Alaska |
genre_facet |
Prudhoe Bay Alaska |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# Aquatic Toxicology Aquatic Toxicology, Volume 95, Issue 3, Page(s) 230-238. http://dx.doi.org/10.1016/j.aquatox.2009.09.006 0166-445X http://hdl.handle.net/11455/68085 doi:10.1016/j.aquatox.2009.09.006 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.aquatox.2009.09.006 |
container_title |
Aquatic Toxicology |
container_volume |
95 |
container_issue |
3 |
container_start_page |
230 |
op_container_end_page |
238 |
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1766174559343476736 |