A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester
BACKGROUND: The focus of this paper is the ultrasound-assisted synthesis of caffeic acid phenethyl ester (CAPE) from caffeic acid and phenyl ethanol in a continuous packed-bed bioreactor. Immobilized Novozym (R) 435 (from Candida antarctica) is used as the catalyst. A three-level-three-factor Box-Be...
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ftnchunghsing:oai:ir.lib.nchu.edu.tw:11455/41100 2023-05-15T13:32:29+02:00 A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester Chen, H.C. 陳建華 Kuo, C.H. Twu, Y.K. Chen, J.H. Chang, C.M.J. Liu, Y.C. Shieh, C.J. 劉永銓 謝淳仁 2011 http://hdl.handle.net/11455/41100 https://doi.org/10.1002/jctb.2661 en_US eng #PLACEHOLDER_PARENT_METADATA_VALUE# Journal of Chemical Technology and Biotechnology Journal of Chemical Technology and Biotechnology, Volume 86, Issue 10, Page(s) 1289-1294. http://dx.doi.org/10.1002/jctb.2661 0268-2575 http://hdl.handle.net/11455/41100 doi:10.1002/jctb.2661 none lipase packed-bed reactor phenolic acid response surface methodology ultrasonication enzymatic-synthesis reactor oil hydrolysis kinetics solvent energy cotton Journal Article 2011 ftnchunghsing https://doi.org/10.1002/jctb.2661 2021-06-26T20:25:19Z BACKGROUND: The focus of this paper is the ultrasound-assisted synthesis of caffeic acid phenethyl ester (CAPE) from caffeic acid and phenyl ethanol in a continuous packed-bed bioreactor. Immobilized Novozym (R) 435 (from Candida antarctica) is used as the catalyst. A three-level-three-factor Box-Behnken design and a response surface methodology (RSM) are employed to evaluate the effects of temperature, flow rate, and ultrasonic power on the percentage molar conversion of CAPE. RESULTS: Based on ridge max analysis, it is concluded that the optimum condition for synthesis is reaction temperature 72.66 degrees C, flow rate 0.046 mL min(-1), and ultrasonic power 1.64 W cm(-2). The expected molar conversion value is 97.84%. An experiment performed under these optimal conditions resulted in a molar conversion of 92.11 +/- 0.75%. The enzyme in the bioreactor was found to be stable for at least 6 days. CONCLUSIONS: The lipase-catalyzed synthesis of CAPE by an ultrasound-assisted packed-bed bioreactor uses mild reaction conditions. Enzymatic synthesis of CAPE is suitable for use in the nutraceutical and food production industries. (C) 2011 Society of Chemical Industry Article in Journal/Newspaper Antarc* Antarctica National Chung Hsing University Institutional Repository - NCHUIR Journal of Chemical Technology & Biotechnology 86 10 1289 1294 |
institution |
Open Polar |
collection |
National Chung Hsing University Institutional Repository - NCHUIR |
op_collection_id |
ftnchunghsing |
language |
English |
topic |
lipase packed-bed reactor phenolic acid response surface methodology ultrasonication enzymatic-synthesis reactor oil hydrolysis kinetics solvent energy cotton |
spellingShingle |
lipase packed-bed reactor phenolic acid response surface methodology ultrasonication enzymatic-synthesis reactor oil hydrolysis kinetics solvent energy cotton Chen, H.C. 陳建華 Kuo, C.H. Twu, Y.K. Chen, J.H. Chang, C.M.J. Liu, Y.C. Shieh, C.J. 劉永銓 謝淳仁 A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester |
topic_facet |
lipase packed-bed reactor phenolic acid response surface methodology ultrasonication enzymatic-synthesis reactor oil hydrolysis kinetics solvent energy cotton |
description |
BACKGROUND: The focus of this paper is the ultrasound-assisted synthesis of caffeic acid phenethyl ester (CAPE) from caffeic acid and phenyl ethanol in a continuous packed-bed bioreactor. Immobilized Novozym (R) 435 (from Candida antarctica) is used as the catalyst. A three-level-three-factor Box-Behnken design and a response surface methodology (RSM) are employed to evaluate the effects of temperature, flow rate, and ultrasonic power on the percentage molar conversion of CAPE. RESULTS: Based on ridge max analysis, it is concluded that the optimum condition for synthesis is reaction temperature 72.66 degrees C, flow rate 0.046 mL min(-1), and ultrasonic power 1.64 W cm(-2). The expected molar conversion value is 97.84%. An experiment performed under these optimal conditions resulted in a molar conversion of 92.11 +/- 0.75%. The enzyme in the bioreactor was found to be stable for at least 6 days. CONCLUSIONS: The lipase-catalyzed synthesis of CAPE by an ultrasound-assisted packed-bed bioreactor uses mild reaction conditions. Enzymatic synthesis of CAPE is suitable for use in the nutraceutical and food production industries. (C) 2011 Society of Chemical Industry |
format |
Article in Journal/Newspaper |
author |
Chen, H.C. 陳建華 Kuo, C.H. Twu, Y.K. Chen, J.H. Chang, C.M.J. Liu, Y.C. Shieh, C.J. 劉永銓 謝淳仁 |
author_facet |
Chen, H.C. 陳建華 Kuo, C.H. Twu, Y.K. Chen, J.H. Chang, C.M.J. Liu, Y.C. Shieh, C.J. 劉永銓 謝淳仁 |
author_sort |
Chen, H.C. |
title |
A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester |
title_short |
A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester |
title_full |
A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester |
title_fullStr |
A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester |
title_full_unstemmed |
A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester |
title_sort |
continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester |
publishDate |
2011 |
url |
http://hdl.handle.net/11455/41100 https://doi.org/10.1002/jctb.2661 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# Journal of Chemical Technology and Biotechnology Journal of Chemical Technology and Biotechnology, Volume 86, Issue 10, Page(s) 1289-1294. http://dx.doi.org/10.1002/jctb.2661 0268-2575 http://hdl.handle.net/11455/41100 doi:10.1002/jctb.2661 |
op_rights |
none |
op_doi |
https://doi.org/10.1002/jctb.2661 |
container_title |
Journal of Chemical Technology & Biotechnology |
container_volume |
86 |
container_issue |
10 |
container_start_page |
1289 |
op_container_end_page |
1294 |
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1766027401864675328 |