Pharmacokinetic and depletion studies of sarafloxacin after oral administration to eel (Anguilla anguilla)

• Published in: Journal of Veterinary Medical Science
• Main Authors: Ho, S.P., 何素鵬, Cheng, C.F., Wang, W.S.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 1999
• Subjects: eel; fish; HPLC; pharmacokinetics; sarafloxacin; salmon salmo-salar; atlantic salmon; rainbow-trout; aeromonas-salmonicida; scophthalmus-maximus; c-14 sarafloxacin; african; catfish; oxolinic acid; sea-water; Anguilla anguilla; Atlantic salmon; Salmo salar; Scophthalmus maximus
• Online Access: http://hdl.handle.net/11455/32695; https://doi.org/10.1292/jvms.61.459

The pharmacokinetics of sarafloxacin applied by oral gavage at a dose of 15 mg/kg b.w. was studied in eel (Anguilla anguilla) at water temperature of 24 degrees C. Sarafloxacin levels were determined using high performance liquid chromatography with a quantitation limit of 0.07 mu g/ml or gram. The time to peak plasma concentration, T-max, was 12 hr and peak concentration, C-max, was 2.64 mu g/ml. The absorption rate constant (k(a)) was 0.23 hr(-1) (r=0.996). The drug disposition curve after T-max was fitted to a two-compartment open model. The distribution rate constant (alpha) was 0.085 hr(-1) (r=0.972), and the half-life (t(1/2 alpha)) was 8.15 hr. The elimination rate constant (beta) was 0.023 hr(-1) (r=0.909), and the half-life (t(1/2 beta), was 30.13 hr. The estimated area under the curve, AUG, was 56.7 mu g.hr/ml. The peak concentrations of drug in liver, kidney, muscle, and skin were 13.39 (12 hr), 5.53 (12 hr), 1.82 (24 hr), and 0.78 mu g/g (40 hr), respectively. The time for sarafloxacin mean levels to fall below detectable limits in the plasma, muscle, and skin were 7 days but for the liver and kidney were 14 days.