Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment
*_Background:_* The metabolic transformation that changes Weddell seal pups born on land into aquatic animals is not only interesting for the study of general biology, but it also provides a model for the acquired and congenital muscle disorders which are associated with oxygen metabolism in skeleta...
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ftnature:oai:nature.com:10101/npre.2009.3380.1 2023-05-15T13:46:06+02:00 Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment Andrey Ptitsyn Shane Kanatous 2009-06-28T22:50:37Z http://precedings.nature.com/documents/3380/version/1 http://hdl.handle.net/10101/npre.2009.3380.1 unknown Creative Commons Attribution 3.0 License CC-BY Nature Precedings Developmental Biology Ecology Bioinformatics Manuscript 2009 ftnature 2015-11-19T12:55:14Z *_Background:_* The metabolic transformation that changes Weddell seal pups born on land into aquatic animals is not only interesting for the study of general biology, but it also provides a model for the acquired and congenital muscle disorders which are associated with oxygen metabolism in skeletal muscle. However, the analysis of gene expression in seals is hampered by the lack of specific microarrays and the very limited annotation of known Weddell seal (_Leptonychotes weddellii_) genes.

*_Results:_* Muscle samples from newborn, juvenile, and adult Weddell seals were collected during an Antarctic expedition. Extracted RNA was hybridized on Affymetrix Human Expression chips. Preliminary studies showed a detectable signal from at least 7000 probe sets present in all samples and replicates. Relative expression levels for these genes was used for further analysis of the biological pathways implicated in the metabolism transformation which occurs in the transition from newborn, to juvenile, to adult seals. Cytoskeletal remodeling, WNT signaling, FAK signaling, hypoxia-induced HIF1 activation, and insulin regulation were identified as being among the most important biological pathways involved in transformation. 

*_Conclusion:_* In spite of certain losses in specificity and sensitivity, the cross-species application of gene expression microarrays is capable of solving challenging puzzles in biology. A Systems Biology approach based on gene interaction patterns can compensate adequately for the lack of species-specific genomics information.
 Manuscript Antarc* Antarctic Weddell Seal Weddell Seals Nature Precedings Antarctic Weddell |
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topic |
Developmental Biology Ecology Bioinformatics |
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Developmental Biology Ecology Bioinformatics Andrey Ptitsyn Shane Kanatous Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
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Developmental Biology Ecology Bioinformatics |
description |
*_Background:_* The metabolic transformation that changes Weddell seal pups born on land into aquatic animals is not only interesting for the study of general biology, but it also provides a model for the acquired and congenital muscle disorders which are associated with oxygen metabolism in skeletal muscle. However, the analysis of gene expression in seals is hampered by the lack of specific microarrays and the very limited annotation of known Weddell seal (_Leptonychotes weddellii_) genes.

*_Results:_* Muscle samples from newborn, juvenile, and adult Weddell seals were collected during an Antarctic expedition. Extracted RNA was hybridized on Affymetrix Human Expression chips. Preliminary studies showed a detectable signal from at least 7000 probe sets present in all samples and replicates. Relative expression levels for these genes was used for further analysis of the biological pathways implicated in the metabolism transformation which occurs in the transition from newborn, to juvenile, to adult seals. Cytoskeletal remodeling, WNT signaling, FAK signaling, hypoxia-induced HIF1 activation, and insulin regulation were identified as being among the most important biological pathways involved in transformation. 

*_Conclusion:_* In spite of certain losses in specificity and sensitivity, the cross-species application of gene expression microarrays is capable of solving challenging puzzles in biology. A Systems Biology approach based on gene interaction patterns can compensate adequately for the lack of species-specific genomics information.
 |
format |
Manuscript |
author |
Andrey Ptitsyn Shane Kanatous |
author_facet |
Andrey Ptitsyn Shane Kanatous |
author_sort |
Andrey Ptitsyn |
title |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_short |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_full |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_fullStr |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_full_unstemmed |
Transformation of metabolism with age and lifestyle in Antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
title_sort |
transformation of metabolism with age and lifestyle in antarctic seals: a case study of systems biology approach to cross-species microarray experiment |
publishDate |
2009 |
url |
http://precedings.nature.com/documents/3380/version/1 http://hdl.handle.net/10101/npre.2009.3380.1 |
geographic |
Antarctic Weddell |
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Antarctic Weddell |
genre |
Antarc* Antarctic Weddell Seal Weddell Seals |
genre_facet |
Antarc* Antarctic Weddell Seal Weddell Seals |
op_source |
Nature Precedings |
op_rights |
Creative Commons Attribution 3.0 License |
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CC-BY |
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1766237017751945216 |