Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway)
A large proportion of reactive nitrogen loss from polar sediments is mediated by denitrification, but microorganisms mediating denitrification in polar environments remain poorly characterized. A combined approach of most-probable-number (MPN) enumeration, cultivation and physiological characterizat...
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ftnasantrs:oai:casi.ntrs.nasa.gov:20140008608 2023-05-15T14:58:46+02:00 Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway) Jahnke, Linda Kostka, Joel E. Kuypers, Marcel M. M. Green, Stefan J. Canion, Andy Prakash, Om Unclassified, Unlimited, Publicly available May 2013 application/pdf http://hdl.handle.net/2060/20140008608 unknown Document ID: 20140008608 http://hdl.handle.net/2060/20140008608 Copyright, Distribution as joint owner in the copyright CASI Life Sciences (General) Oceanography ARC-E-DAA-TN13900 2013 ftnasantrs 2019-07-21T06:14:43Z A large proportion of reactive nitrogen loss from polar sediments is mediated by denitrification, but microorganisms mediating denitrification in polar environments remain poorly characterized. A combined approach of most-probable-number (MPN) enumeration, cultivation and physiological characterization was used to describe psychrophilic denitrifying bacterial communities in sediments of three Arctic fjords in Svalbard (Norway). A MPN assay showed the presence of 10(sup 3)10(sup 6) cells of psychrophilic nitrate-respiring bacteria g(sup 1) of sediment. Fifteen strains within the Proteobacteria were isolated using a systematic enrichment approach with organic acids as electron donors and nitrate as an electron acceptor. Isolates belonged to five genera, including Shewanella, Pseudomonas, Psychromonas (Gammaproteobacteria), Arcobacter (Epsilonproteobacteria) and Herminiimonas (Betaproteobacteria). All isolates were denitrifiers, except Shewanella, which exhibited the capacity for dissimilatory nitrate reduction to ammonium (DNRA). Growth from 0 to 40 degC demonstrated that all genera except Shewanella were psychrophiles with optimal growth below 15 degC, and adaptation to low temperature was demonstrated as a shift from primarily C16:0 saturated fatty acids to C16:1 monounsaturated fatty acids at lower temperatures. This study provides the first targeted enrichment and characterization of psychrophilic denitrifying bacteria from polar sediments, and two genera, Arcobacter and Herminiimonas, are isolated for the first time from permanently cold marine sediments. Other/Unknown Material Arctic Svalbard NASA Technical Reports Server (NTRS) Arctic Svalbard Norway |
institution |
Open Polar |
collection |
NASA Technical Reports Server (NTRS) |
op_collection_id |
ftnasantrs |
language |
unknown |
topic |
Life Sciences (General) Oceanography |
spellingShingle |
Life Sciences (General) Oceanography Jahnke, Linda Kostka, Joel E. Kuypers, Marcel M. M. Green, Stefan J. Canion, Andy Prakash, Om Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway) |
topic_facet |
Life Sciences (General) Oceanography |
description |
A large proportion of reactive nitrogen loss from polar sediments is mediated by denitrification, but microorganisms mediating denitrification in polar environments remain poorly characterized. A combined approach of most-probable-number (MPN) enumeration, cultivation and physiological characterization was used to describe psychrophilic denitrifying bacterial communities in sediments of three Arctic fjords in Svalbard (Norway). A MPN assay showed the presence of 10(sup 3)10(sup 6) cells of psychrophilic nitrate-respiring bacteria g(sup 1) of sediment. Fifteen strains within the Proteobacteria were isolated using a systematic enrichment approach with organic acids as electron donors and nitrate as an electron acceptor. Isolates belonged to five genera, including Shewanella, Pseudomonas, Psychromonas (Gammaproteobacteria), Arcobacter (Epsilonproteobacteria) and Herminiimonas (Betaproteobacteria). All isolates were denitrifiers, except Shewanella, which exhibited the capacity for dissimilatory nitrate reduction to ammonium (DNRA). Growth from 0 to 40 degC demonstrated that all genera except Shewanella were psychrophiles with optimal growth below 15 degC, and adaptation to low temperature was demonstrated as a shift from primarily C16:0 saturated fatty acids to C16:1 monounsaturated fatty acids at lower temperatures. This study provides the first targeted enrichment and characterization of psychrophilic denitrifying bacteria from polar sediments, and two genera, Arcobacter and Herminiimonas, are isolated for the first time from permanently cold marine sediments. |
format |
Other/Unknown Material |
author |
Jahnke, Linda Kostka, Joel E. Kuypers, Marcel M. M. Green, Stefan J. Canion, Andy Prakash, Om |
author_facet |
Jahnke, Linda Kostka, Joel E. Kuypers, Marcel M. M. Green, Stefan J. Canion, Andy Prakash, Om |
author_sort |
Jahnke, Linda |
title |
Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway) |
title_short |
Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway) |
title_full |
Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway) |
title_fullStr |
Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway) |
title_full_unstemmed |
Isolation and Physiological Characterization of Psychrophilic Denitrifying Bacteria from Permanently Cold Arctic Fjord Sediments (Svalbard, Norway) |
title_sort |
isolation and physiological characterization of psychrophilic denitrifying bacteria from permanently cold arctic fjord sediments (svalbard, norway) |
publishDate |
2013 |
url |
http://hdl.handle.net/2060/20140008608 |
op_coverage |
Unclassified, Unlimited, Publicly available |
geographic |
Arctic Svalbard Norway |
geographic_facet |
Arctic Svalbard Norway |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_source |
CASI |
op_relation |
Document ID: 20140008608 http://hdl.handle.net/2060/20140008608 |
op_rights |
Copyright, Distribution as joint owner in the copyright |
_version_ |
1766330899623837696 |