Telomere length measurement by qPCR in birds is affected by storage method of blood samples
Given the potential role of telomeres as biomarkers of individual health and ageing, there is an increasing interest in studying telomere dynamics in a wider range of taxa in the fields of ecology and evolutionary biology. Measuring telomere length across the lifespan in wild animal systems is essen...
Published in: | Oecologia |
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2017
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Online Access: | https://doi.org/10.1007/s00442-017-3887-3 https://napier-repository.worktribe.com/file/2810017/1/Telomere%20Length%20Measurement%20By%20QPCR%20In%20Birds%20Is%20Affected%20By%20Storage%20Method%20Of%20Blood%20Samples http://researchrepository.napier.ac.uk/Output/2810017 |
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ftnapieruniv:oai:repository@napier.ac.uk:2810017 2023-05-15T18:43:03+02:00 Telomere length measurement by qPCR in birds is affected by storage method of blood samples Reichert, Sophie Froy, Hannah Boner, Winnie Burg, Theresa M. Daunt, Francis Gillespie, Robert Griffiths, Kate Lewis, Sue Phillips, Richard A. Nussey, Dan H. Monaghan, Pat 2017-05-25 https://doi.org/10.1007/s00442-017-3887-3 https://napier-repository.worktribe.com/file/2810017/1/Telomere%20Length%20Measurement%20By%20QPCR%20In%20Birds%20Is%20Affected%20By%20Storage%20Method%20Of%20Blood%20Samples http://researchrepository.napier.ac.uk/Output/2810017 unknown Springer http://researchrepository.napier.ac.uk/Output/2810017 doi:https://doi.org/10.1007/s00442-017-3887-3 https://napier-repository.worktribe.com/file/2810017/1/Telomere%20Length%20Measurement%20By%20QPCR%20In%20Birds%20Is%20Affected%20By%20Storage%20Method%20Of%20Blood%20Samples 0029-8549 10.1007/s00442-017-3887-3 openAccess http://creativecommons.org/licenses/by/4.0/ CC-BY Wandering albatross Zebra finch FTA cards DNA Telomeres Ageing Long-term field study Journal Article publishedVersion 2017 ftnapieruniv https://doi.org/10.1007/s00442-017-3887-3 2022-10-13T22:43:24Z Given the potential role of telomeres as biomarkers of individual health and ageing, there is an increasing interest in studying telomere dynamics in a wider range of taxa in the fields of ecology and evolutionary biology. Measuring telomere length across the lifespan in wild animal systems is essential for testing these hypotheses, and may be aided by archived blood samples collected as part of longitudinal field studies. However, sample collection, storage, and DNA extraction methods may influence telomere length measurement, and it may, therefore, be difficult to balance consistency in sampling protocol with making the most of available samples. We used two complementary approaches to examine the impacts of sample storage method on measurements of relative telomere length (RTL) by qPCR, particularly focusing on FTA (Flinders Technology Associates) cards as a long-term storage solution. We used blood samples from wandering albatrosses collected over 14 years and stored in three different ways (n = 179), and also blood samples from captive zebra finches (n = 30) that were each stored using three different methods. Sample storage method influenced RTL in both studies, and samples on FTA cards had significantly shorter RTL measurements. There was no significant correlation between RTL measured in zebra finch blood on FTA cards and the same samples stored either as frozen whole blood or as extracted DNA. These results highlight the importance of consistency of sampling protocol, particularly in the context of long-term field studies, and suggest that FTA cards should not be used as a long-term storage solution to measure RTL without validation. Article in Journal/Newspaper Wandering Albatross Edinburgh Napier Repository (Napier University Edinburgh) Finch ENVELOPE(167.383,167.383,-72.567,-72.567) Flinders ENVELOPE(-66.667,-66.667,-69.267,-69.267) Oecologia 184 2 341 350 |
institution |
Open Polar |
collection |
Edinburgh Napier Repository (Napier University Edinburgh) |
op_collection_id |
ftnapieruniv |
language |
unknown |
topic |
Wandering albatross Zebra finch FTA cards DNA Telomeres Ageing Long-term field study |
spellingShingle |
Wandering albatross Zebra finch FTA cards DNA Telomeres Ageing Long-term field study Reichert, Sophie Froy, Hannah Boner, Winnie Burg, Theresa M. Daunt, Francis Gillespie, Robert Griffiths, Kate Lewis, Sue Phillips, Richard A. Nussey, Dan H. Monaghan, Pat Telomere length measurement by qPCR in birds is affected by storage method of blood samples |
topic_facet |
Wandering albatross Zebra finch FTA cards DNA Telomeres Ageing Long-term field study |
description |
Given the potential role of telomeres as biomarkers of individual health and ageing, there is an increasing interest in studying telomere dynamics in a wider range of taxa in the fields of ecology and evolutionary biology. Measuring telomere length across the lifespan in wild animal systems is essential for testing these hypotheses, and may be aided by archived blood samples collected as part of longitudinal field studies. However, sample collection, storage, and DNA extraction methods may influence telomere length measurement, and it may, therefore, be difficult to balance consistency in sampling protocol with making the most of available samples. We used two complementary approaches to examine the impacts of sample storage method on measurements of relative telomere length (RTL) by qPCR, particularly focusing on FTA (Flinders Technology Associates) cards as a long-term storage solution. We used blood samples from wandering albatrosses collected over 14 years and stored in three different ways (n = 179), and also blood samples from captive zebra finches (n = 30) that were each stored using three different methods. Sample storage method influenced RTL in both studies, and samples on FTA cards had significantly shorter RTL measurements. There was no significant correlation between RTL measured in zebra finch blood on FTA cards and the same samples stored either as frozen whole blood or as extracted DNA. These results highlight the importance of consistency of sampling protocol, particularly in the context of long-term field studies, and suggest that FTA cards should not be used as a long-term storage solution to measure RTL without validation. |
format |
Article in Journal/Newspaper |
author |
Reichert, Sophie Froy, Hannah Boner, Winnie Burg, Theresa M. Daunt, Francis Gillespie, Robert Griffiths, Kate Lewis, Sue Phillips, Richard A. Nussey, Dan H. Monaghan, Pat |
author_facet |
Reichert, Sophie Froy, Hannah Boner, Winnie Burg, Theresa M. Daunt, Francis Gillespie, Robert Griffiths, Kate Lewis, Sue Phillips, Richard A. Nussey, Dan H. Monaghan, Pat |
author_sort |
Reichert, Sophie |
title |
Telomere length measurement by qPCR in birds is affected by storage method of blood samples |
title_short |
Telomere length measurement by qPCR in birds is affected by storage method of blood samples |
title_full |
Telomere length measurement by qPCR in birds is affected by storage method of blood samples |
title_fullStr |
Telomere length measurement by qPCR in birds is affected by storage method of blood samples |
title_full_unstemmed |
Telomere length measurement by qPCR in birds is affected by storage method of blood samples |
title_sort |
telomere length measurement by qpcr in birds is affected by storage method of blood samples |
publisher |
Springer |
publishDate |
2017 |
url |
https://doi.org/10.1007/s00442-017-3887-3 https://napier-repository.worktribe.com/file/2810017/1/Telomere%20Length%20Measurement%20By%20QPCR%20In%20Birds%20Is%20Affected%20By%20Storage%20Method%20Of%20Blood%20Samples http://researchrepository.napier.ac.uk/Output/2810017 |
long_lat |
ENVELOPE(167.383,167.383,-72.567,-72.567) ENVELOPE(-66.667,-66.667,-69.267,-69.267) |
geographic |
Finch Flinders |
geographic_facet |
Finch Flinders |
genre |
Wandering Albatross |
genre_facet |
Wandering Albatross |
op_relation |
http://researchrepository.napier.ac.uk/Output/2810017 doi:https://doi.org/10.1007/s00442-017-3887-3 https://napier-repository.worktribe.com/file/2810017/1/Telomere%20Length%20Measurement%20By%20QPCR%20In%20Birds%20Is%20Affected%20By%20Storage%20Method%20Of%20Blood%20Samples 0029-8549 10.1007/s00442-017-3887-3 |
op_rights |
openAccess http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00442-017-3887-3 |
container_title |
Oecologia |
container_volume |
184 |
container_issue |
2 |
container_start_page |
341 |
op_container_end_page |
350 |
_version_ |
1766232838987841536 |