Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil
Arctic ecosystems are under pressure from climate change and atmospheric nitrogen (N) deposition. However, knowledge of the ecology of microbial communities and their responses to such challenges in Arctic tundra soil remain limited, despite the central role these organisms play for ecosystem functi...
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Online Access: | https://doi.org/10.1007/s00300-015-1661-8 https://napier-repository.worktribe.com/file/1348576/1/Variation%20in%20bacterial,%20archaeal%20and%20fungal%20community%20structure%20and%20abundance%20in%20High%20Arctic%20tundra%20soil http://researchrepository.napier.ac.uk/Output/1348576 |
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ftnapieruniv:oai:repository@napier.ac.uk:1348576 2024-04-28T08:07:29+00:00 Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil Blaud, Aimeric Phoenix, Gareth K. Osborn, A. Mark 2015-02-22 https://doi.org/10.1007/s00300-015-1661-8 https://napier-repository.worktribe.com/file/1348576/1/Variation%20in%20bacterial,%20archaeal%20and%20fungal%20community%20structure%20and%20abundance%20in%20High%20Arctic%20tundra%20soil http://researchrepository.napier.ac.uk/Output/1348576 unknown BMC http://researchrepository.napier.ac.uk/Output/1348576 doi:https://doi.org/10.1007/s00300-015-1661-8 https://napier-repository.worktribe.com/file/1348576/1/Variation%20in%20bacterial,%20archaeal%20and%20fungal%20community%20structure%20and%20abundance%20in%20High%20Arctic%20tundra%20soil 0722-4060 10.1007/s00300-015-1661-8 openAccess Svalbard Soil horizons Environmental drivers Atmospheric nitrogen deposition T-RFLP ARISA Q-PCR Journal Article acceptedVersion publishedVersion 2015 ftnapieruniv https://doi.org/10.1007/s00300-015-1661-8 2024-04-09T23:45:06Z Arctic ecosystems are under pressure from climate change and atmospheric nitrogen (N) deposition. However, knowledge of the ecology of microbial communities and their responses to such challenges in Arctic tundra soil remain limited, despite the central role these organisms play for ecosystem functioning. We utilised a plot-scale experiment in High Arctic tundra on Svalbard to investigate short-term variation (9 days), following simulation of a N deposition event (4 kg N ha−1 yr−1), in the structure and abundance of bacterial, archaeal and fungal communities between organic and mineral soil horizons. T-RFLP analysis showed significant differences between horizons in bacterial and archaeal community structure. Q-PCR analysis showed that fungal abundance did not differ significantly between soil horizons, whilst bacterial and archaeal abundance was significantly higher in mineral than in organic horizons, despite soil water and total C and N contents being significantly greater in the organic horizon. In the organic horizon, bacterial community structure and fungal abundance varied significantly over time. In the mineral horizon, there was significant variation over time in bacterial abundance, in archaeal community structure and in both fungal community structure and abundance. In contrast, N deposition did not lead to significant variation in either the structure or the abundance of microbial communities. This research demonstrates that microbial community structure and abundance can change rapidly (over only a few days) in Arctic tundra soils and also differently between soil horizons in response to different environmental drivers. Moreover, this variability in microbial community structure and abundance is soil horizon- and taxonomic domain-specific, highlighting the importance of investigating microbial communities across all soil horizons and over short periods of time. Article in Journal/Newspaper Arctic Climate change Svalbard Tundra Edinburgh Napier Repository (Napier University Edinburgh) Polar Biology 38 7 1009 1024 |
institution |
Open Polar |
collection |
Edinburgh Napier Repository (Napier University Edinburgh) |
op_collection_id |
ftnapieruniv |
language |
unknown |
topic |
Svalbard Soil horizons Environmental drivers Atmospheric nitrogen deposition T-RFLP ARISA Q-PCR |
spellingShingle |
Svalbard Soil horizons Environmental drivers Atmospheric nitrogen deposition T-RFLP ARISA Q-PCR Blaud, Aimeric Phoenix, Gareth K. Osborn, A. Mark Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil |
topic_facet |
Svalbard Soil horizons Environmental drivers Atmospheric nitrogen deposition T-RFLP ARISA Q-PCR |
description |
Arctic ecosystems are under pressure from climate change and atmospheric nitrogen (N) deposition. However, knowledge of the ecology of microbial communities and their responses to such challenges in Arctic tundra soil remain limited, despite the central role these organisms play for ecosystem functioning. We utilised a plot-scale experiment in High Arctic tundra on Svalbard to investigate short-term variation (9 days), following simulation of a N deposition event (4 kg N ha−1 yr−1), in the structure and abundance of bacterial, archaeal and fungal communities between organic and mineral soil horizons. T-RFLP analysis showed significant differences between horizons in bacterial and archaeal community structure. Q-PCR analysis showed that fungal abundance did not differ significantly between soil horizons, whilst bacterial and archaeal abundance was significantly higher in mineral than in organic horizons, despite soil water and total C and N contents being significantly greater in the organic horizon. In the organic horizon, bacterial community structure and fungal abundance varied significantly over time. In the mineral horizon, there was significant variation over time in bacterial abundance, in archaeal community structure and in both fungal community structure and abundance. In contrast, N deposition did not lead to significant variation in either the structure or the abundance of microbial communities. This research demonstrates that microbial community structure and abundance can change rapidly (over only a few days) in Arctic tundra soils and also differently between soil horizons in response to different environmental drivers. Moreover, this variability in microbial community structure and abundance is soil horizon- and taxonomic domain-specific, highlighting the importance of investigating microbial communities across all soil horizons and over short periods of time. |
format |
Article in Journal/Newspaper |
author |
Blaud, Aimeric Phoenix, Gareth K. Osborn, A. Mark |
author_facet |
Blaud, Aimeric Phoenix, Gareth K. Osborn, A. Mark |
author_sort |
Blaud, Aimeric |
title |
Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil |
title_short |
Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil |
title_full |
Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil |
title_fullStr |
Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil |
title_full_unstemmed |
Variation in bacterial, archaeal and fungal community structure and abundance in High Arctic tundra soil |
title_sort |
variation in bacterial, archaeal and fungal community structure and abundance in high arctic tundra soil |
publisher |
BMC |
publishDate |
2015 |
url |
https://doi.org/10.1007/s00300-015-1661-8 https://napier-repository.worktribe.com/file/1348576/1/Variation%20in%20bacterial,%20archaeal%20and%20fungal%20community%20structure%20and%20abundance%20in%20High%20Arctic%20tundra%20soil http://researchrepository.napier.ac.uk/Output/1348576 |
genre |
Arctic Climate change Svalbard Tundra |
genre_facet |
Arctic Climate change Svalbard Tundra |
op_relation |
http://researchrepository.napier.ac.uk/Output/1348576 doi:https://doi.org/10.1007/s00300-015-1661-8 https://napier-repository.worktribe.com/file/1348576/1/Variation%20in%20bacterial,%20archaeal%20and%20fungal%20community%20structure%20and%20abundance%20in%20High%20Arctic%20tundra%20soil 0722-4060 10.1007/s00300-015-1661-8 |
op_rights |
openAccess |
op_doi |
https://doi.org/10.1007/s00300-015-1661-8 |
container_title |
Polar Biology |
container_volume |
38 |
container_issue |
7 |
container_start_page |
1009 |
op_container_end_page |
1024 |
_version_ |
1797576585639886848 |