日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について
Nagasaki University (長崎大学) 博士(医学) Due to the increase in the number of azole-resistant Aspergillus fumigatus, there is an urgent need of data to predict future trends and prevent further spreading. The intercountry transfer of resistant A. fumigatus on plant bulbs have been reported. We investigated...
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ftnagasakiuniv:oai:nagasaki-u.repo.nii.ac.jp:00027898 2024-09-15T18:38:44+00:00 日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について Characteristics of azole-resistant Aspergillus fumigatus attached to agricultural products imported to Japan 中野, 裕一郎 2022-12-07 application/pdf https://nagasaki-u.repo.nii.ac.jp/record/27898/files/ISYK1478_Nakano.pdf eng eng Elsevier Ltd. 10.1016/j.jiac.2020.05.008 http://hdl.handle.net/10069/00041985 Journal of Infection and Chemotherapy 10 26 1021 1025 甲医歯薬第1478号 1341-321X https://nagasaki-u.repo.nii.ac.jp/record/27898/files/ISYK1478_Nakano.pdf © 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved. open access Aspergillus fumigatus Azole resistance Agricultural imports Japan VoR 2022 ftnagasakiuniv 2024-08-23T06:19:41Z Nagasaki University (長崎大学) 博士(医学) Due to the increase in the number of azole-resistant Aspergillus fumigatus, there is an urgent need of data to predict future trends and prevent further spreading. The intercountry transfer of resistant A. fumigatus on plant bulbs have been reported. We investigated existence and characteristics of resistant isolates attached to agricultural products imported to Japan. We purchased 292 samples in Japan. All samples were screened for the existence of azole-resistant A. fumigatus. For positive isolates, minimum inhibitory concentrations of the drugs were determined. We also analyzed Cyp51A, Hmg1, and Erg6 mutations of these isolates and conducted microsatellite genotyping. Fourteen azole-resistant isolates were detected, of which 13 were cultured from flower bulbs imported from the Netherlands. Among them 5 were from 11 bulbs of Hippeastrum (45.5%), 5 were from 24 bulbs of Gladiolus (20.8%), 2 were from 4 bulbs of Ixia (50.0%), and 1 was from 22 bulbs of Tulipa (4.5%). Only 1 resistant isolate was cultured from the 10 bulbs of Narcissus (10.0%) originating in Japan. Various novel mutations including Y121F/T289A in Cyp51A with no tandem repeat in promoter region were discovered from imported strains. Our study provides important data showing that agricultural imports provide a possible route for their intercontinental spread and raises the concern that strains harboring highly diverse Cyp51A mutations might increase in clinical settings in the future. 長崎大学学位論文 学位記番号:博(医歯薬)甲第1478号 学位授与年月日:令和4年12月7日 Author: Yuichiro Nakano, Masato Tashiro, Ryo Urano, Minori Kikuchi, Naoki Ito, Eriko Moriya, Tomoyuki Shirahige, Maki Mishima, Takahiro Takazono, Taiga Miyazaki, Koichi Izumikawa Citation: Journal of Infection and Chemotherapy, 26(10), pp.1021-1025; 2020 Nagasaki University (長崎大学), 博士(医学) (2022-12-07) doctoral thesis Other/Unknown Material taiga NAOSITE: Nagasaki University Academic Output SITE |
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English |
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Aspergillus fumigatus Azole resistance Agricultural imports Japan |
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Aspergillus fumigatus Azole resistance Agricultural imports Japan 中野, 裕一郎 日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について |
topic_facet |
Aspergillus fumigatus Azole resistance Agricultural imports Japan |
description |
Nagasaki University (長崎大学) 博士(医学) Due to the increase in the number of azole-resistant Aspergillus fumigatus, there is an urgent need of data to predict future trends and prevent further spreading. The intercountry transfer of resistant A. fumigatus on plant bulbs have been reported. We investigated existence and characteristics of resistant isolates attached to agricultural products imported to Japan. We purchased 292 samples in Japan. All samples were screened for the existence of azole-resistant A. fumigatus. For positive isolates, minimum inhibitory concentrations of the drugs were determined. We also analyzed Cyp51A, Hmg1, and Erg6 mutations of these isolates and conducted microsatellite genotyping. Fourteen azole-resistant isolates were detected, of which 13 were cultured from flower bulbs imported from the Netherlands. Among them 5 were from 11 bulbs of Hippeastrum (45.5%), 5 were from 24 bulbs of Gladiolus (20.8%), 2 were from 4 bulbs of Ixia (50.0%), and 1 was from 22 bulbs of Tulipa (4.5%). Only 1 resistant isolate was cultured from the 10 bulbs of Narcissus (10.0%) originating in Japan. Various novel mutations including Y121F/T289A in Cyp51A with no tandem repeat in promoter region were discovered from imported strains. Our study provides important data showing that agricultural imports provide a possible route for their intercontinental spread and raises the concern that strains harboring highly diverse Cyp51A mutations might increase in clinical settings in the future. 長崎大学学位論文 学位記番号:博(医歯薬)甲第1478号 学位授与年月日:令和4年12月7日 Author: Yuichiro Nakano, Masato Tashiro, Ryo Urano, Minori Kikuchi, Naoki Ito, Eriko Moriya, Tomoyuki Shirahige, Maki Mishima, Takahiro Takazono, Taiga Miyazaki, Koichi Izumikawa Citation: Journal of Infection and Chemotherapy, 26(10), pp.1021-1025; 2020 Nagasaki University (長崎大学), 博士(医学) (2022-12-07) doctoral thesis |
format |
Other/Unknown Material |
author |
中野, 裕一郎 |
author_facet |
中野, 裕一郎 |
author_sort |
中野, 裕一郎 |
title |
日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について |
title_short |
日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について |
title_full |
日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について |
title_fullStr |
日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について |
title_full_unstemmed |
日本への輸入農産物に付着したアゾール耐性Aspergillus fumigatusの特徴について |
title_sort |
日本への輸入農産物に付着したアゾール耐性aspergillus fumigatusの特徴について |
publisher |
Elsevier Ltd. |
publishDate |
2022 |
url |
https://nagasaki-u.repo.nii.ac.jp/record/27898/files/ISYK1478_Nakano.pdf |
genre |
taiga |
genre_facet |
taiga |
op_relation |
10.1016/j.jiac.2020.05.008 http://hdl.handle.net/10069/00041985 Journal of Infection and Chemotherapy 10 26 1021 1025 甲医歯薬第1478号 1341-321X https://nagasaki-u.repo.nii.ac.jp/record/27898/files/ISYK1478_Nakano.pdf |
op_rights |
© 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved. open access |
_version_ |
1810483123554615296 |