Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder
Streptococcus parauberis is a pathogen of streptococcosis in turbot Scophthalmus maximus and the Japanese flounder Paralichthys olivaceus. S. parauberis isolates from diseased Japanese flounder have been classified into several serological phenotypes. In this study we analyzed the DNA sequence of th...
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日本魚病学会
2015
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ftnagasakiuniv:oai:nagasaki-u.repo.nii.ac.jp:00003510 2024-09-15T18:34:01+00:00 Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder Tu, Chuandeng Suga, Koushirou Kanai, Kinya 2015-12-29 application/pdf https://nagasaki-u.repo.nii.ac.jp/record/3510/files/JSFP50_192.pdf eng eng 日本魚病学会 10.3147/jsfp.50.192 Fish Pathology 4 50 192 199 0388788X 18817335 https://nagasaki-u.repo.nii.ac.jp/record/3510/files/JSFP50_192.pdf c 2015 The Japanese Society of Fish Pathology Streptococcus parauberis streptococcosis serotype capsular polysaccharide biosynthesis cps locus structure Paralichthys olivaceus VoR 2015 ftnagasakiuniv 2024-08-23T06:19:41Z Streptococcus parauberis is a pathogen of streptococcosis in turbot Scophthalmus maximus and the Japanese flounder Paralichthys olivaceus. S. parauberis isolates from diseased Japanese flounder have been classified into several serological phenotypes. In this study we analyzed the DNA sequence of the genetic locus for capsular polysaccharide (CPS) biosynthesis of S. parauberis KRS02083 (subserotype Ia), NUF1003 (subserotype Ib), NUF1071 (subserotype Ic), NUF1032 (serotype II), 2007-1 (nontypeable/PFGE cluster I) and NUF1095 (nontypeable/PFGE cluster III) to elucidate the genetic basis for serological diversity. As a result, three kinds of cps locus were revealed among serotypes and subserotypes, namely the loci for subserotype Ia, subserotypes Ib/Ic and serotype II. The genetic structure of cps loci suggests that the capsules of S. parauberis are synthesized through the Wzy-dependent pathway. Subserotypes Ib and Ic possessed the same genetic structure, although single-base substitution at several regions or insertion of an IS (insertion sequence) element was found in subserotype Ic. The nontypeable strains, which agglutinated with both serotypes I and II antisera, possessed the same genetic structure as subserotype Ib/Ic or serotype II with single-base substitution at several regions. Fish Pathology, 50(4), pp.192-199; 2015 journal article Other/Unknown Material Scophthalmus maximus Turbot NAOSITE: Nagasaki University Academic Output SITE |
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Open Polar |
collection |
NAOSITE: Nagasaki University Academic Output SITE |
op_collection_id |
ftnagasakiuniv |
language |
English |
topic |
Streptococcus parauberis streptococcosis serotype capsular polysaccharide biosynthesis cps locus structure Paralichthys olivaceus |
spellingShingle |
Streptococcus parauberis streptococcosis serotype capsular polysaccharide biosynthesis cps locus structure Paralichthys olivaceus Tu, Chuandeng Suga, Koushirou Kanai, Kinya Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder |
topic_facet |
Streptococcus parauberis streptococcosis serotype capsular polysaccharide biosynthesis cps locus structure Paralichthys olivaceus |
description |
Streptococcus parauberis is a pathogen of streptococcosis in turbot Scophthalmus maximus and the Japanese flounder Paralichthys olivaceus. S. parauberis isolates from diseased Japanese flounder have been classified into several serological phenotypes. In this study we analyzed the DNA sequence of the genetic locus for capsular polysaccharide (CPS) biosynthesis of S. parauberis KRS02083 (subserotype Ia), NUF1003 (subserotype Ib), NUF1071 (subserotype Ic), NUF1032 (serotype II), 2007-1 (nontypeable/PFGE cluster I) and NUF1095 (nontypeable/PFGE cluster III) to elucidate the genetic basis for serological diversity. As a result, three kinds of cps locus were revealed among serotypes and subserotypes, namely the loci for subserotype Ia, subserotypes Ib/Ic and serotype II. The genetic structure of cps loci suggests that the capsules of S. parauberis are synthesized through the Wzy-dependent pathway. Subserotypes Ib and Ic possessed the same genetic structure, although single-base substitution at several regions or insertion of an IS (insertion sequence) element was found in subserotype Ic. The nontypeable strains, which agglutinated with both serotypes I and II antisera, possessed the same genetic structure as subserotype Ib/Ic or serotype II with single-base substitution at several regions. Fish Pathology, 50(4), pp.192-199; 2015 journal article |
format |
Other/Unknown Material |
author |
Tu, Chuandeng Suga, Koushirou Kanai, Kinya |
author_facet |
Tu, Chuandeng Suga, Koushirou Kanai, Kinya |
author_sort |
Tu, Chuandeng |
title |
Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder |
title_short |
Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder |
title_full |
Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder |
title_fullStr |
Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder |
title_full_unstemmed |
Structure of Genetic Loci for Capsular Polysaccharide Biosynthesis in Streptococcus parauberis isolated from Japanese flounder |
title_sort |
structure of genetic loci for capsular polysaccharide biosynthesis in streptococcus parauberis isolated from japanese flounder |
publisher |
日本魚病学会 |
publishDate |
2015 |
url |
https://nagasaki-u.repo.nii.ac.jp/record/3510/files/JSFP50_192.pdf |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
10.3147/jsfp.50.192 Fish Pathology 4 50 192 199 0388788X 18817335 https://nagasaki-u.repo.nii.ac.jp/record/3510/files/JSFP50_192.pdf |
op_rights |
c 2015 The Japanese Society of Fish Pathology |
_version_ |
1810475747847962624 |