Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia)
Place: Edinburgh Publisher: Churchill Livingstone WOS:000522853900012 International audience Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were...
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Online Access: | https://hal.umontpellier.fr/hal-03411067 https://hal.umontpellier.fr/hal-03411067/document https://hal.umontpellier.fr/hal-03411067/file/S0040816619305075.pdf https://doi.org/10.1016/j.tice.2020.101340 |
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ftmuseumnhn:oai:HAL:hal-03411067v1 2024-04-14T08:12:48+00:00 Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) Sellami, Imene Charmantier, Guy Naceur, Hachem B. Kacem, Adnane Lorin-Nebel, Catherine MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Institut Supérieur de Biotechnologie de Monastir (ISBM) Université de Monastir - University of Monastir (UM) Université de Montpellier (UM) 2020 https://hal.umontpellier.fr/hal-03411067 https://hal.umontpellier.fr/hal-03411067/document https://hal.umontpellier.fr/hal-03411067/file/S0040816619305075.pdf https://doi.org/10.1016/j.tice.2020.101340 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tice.2020.101340 hal-03411067 https://hal.umontpellier.fr/hal-03411067 https://hal.umontpellier.fr/hal-03411067/document https://hal.umontpellier.fr/hal-03411067/file/S0040816619305075.pdf doi:10.1016/j.tice.2020.101340 PII: S0040-8166(19)30507-5 http://creativecommons.org/licenses/by-nc/ info:eu-repo/semantics/OpenAccess ISSN: 0040-8166 Tissue and Cell https://hal.umontpellier.fr/hal-03411067 Tissue and Cell, 2020, 63, pp.UNSP 101340. ⟨10.1016/j.tice.2020.101340⟩ ontogeny fresh-water alpha-subunit Artemia salina brine shrimp artemia homarus-gammarus crustacea Hypo-osmoregulation Immunolocalization ionic regulation Maxillary glands Metepipodites na+ k+-atpase Na+/K+ -ATPase osmotic regulation potential difference salt-gland [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes info:eu-repo/semantics/article Journal articles 2020 ftmuseumnhn https://doi.org/10.1016/j.tice.2020.101340 2024-03-21T16:33:33Z Place: Edinburgh Publisher: Churchill Livingstone WOS:000522853900012 International audience Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was observed in the epithelium of the ten pairs of metepipodites. A positive immunoreactivity for Na+/K+-ATPase was also detected in the maxillary glands, in the epithelium of the efferent tubule and of the excretory canal, as well as in the anterior digestive tract. This study confirms the strong hypo-osmotic capacity of this species and affords an overview of the different organs involved in osmoregulation in A. salina adults. Article in Journal/Newspaper Homarus gammarus Muséum National d'Histoire Naturelle (MNHM): HAL Hani ENVELOPE(-20.331,-20.331,63.441,63.441) Livingstone ENVELOPE(-134.337,-134.337,61.333,61.333) Tissue and Cell 63 101340 |
institution |
Open Polar |
collection |
Muséum National d'Histoire Naturelle (MNHM): HAL |
op_collection_id |
ftmuseumnhn |
language |
English |
topic |
ontogeny fresh-water alpha-subunit Artemia salina brine shrimp artemia homarus-gammarus crustacea Hypo-osmoregulation Immunolocalization ionic regulation Maxillary glands Metepipodites na+ k+-atpase Na+/K+ -ATPase osmotic regulation potential difference salt-gland [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes |
spellingShingle |
ontogeny fresh-water alpha-subunit Artemia salina brine shrimp artemia homarus-gammarus crustacea Hypo-osmoregulation Immunolocalization ionic regulation Maxillary glands Metepipodites na+ k+-atpase Na+/K+ -ATPase osmotic regulation potential difference salt-gland [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes Sellami, Imene Charmantier, Guy Naceur, Hachem B. Kacem, Adnane Lorin-Nebel, Catherine Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) |
topic_facet |
ontogeny fresh-water alpha-subunit Artemia salina brine shrimp artemia homarus-gammarus crustacea Hypo-osmoregulation Immunolocalization ionic regulation Maxillary glands Metepipodites na+ k+-atpase Na+/K+ -ATPase osmotic regulation potential difference salt-gland [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes |
description |
Place: Edinburgh Publisher: Churchill Livingstone WOS:000522853900012 International audience Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was observed in the epithelium of the ten pairs of metepipodites. A positive immunoreactivity for Na+/K+-ATPase was also detected in the maxillary glands, in the epithelium of the efferent tubule and of the excretory canal, as well as in the anterior digestive tract. This study confirms the strong hypo-osmotic capacity of this species and affords an overview of the different organs involved in osmoregulation in A. salina adults. |
author2 |
MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Institut Supérieur de Biotechnologie de Monastir (ISBM) Université de Monastir - University of Monastir (UM) Université de Montpellier (UM) |
format |
Article in Journal/Newspaper |
author |
Sellami, Imene Charmantier, Guy Naceur, Hachem B. Kacem, Adnane Lorin-Nebel, Catherine |
author_facet |
Sellami, Imene Charmantier, Guy Naceur, Hachem B. Kacem, Adnane Lorin-Nebel, Catherine |
author_sort |
Sellami, Imene |
title |
Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) |
title_short |
Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) |
title_full |
Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) |
title_fullStr |
Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) |
title_full_unstemmed |
Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia) |
title_sort |
osmoregulatory performance and immunolocalization of na+/k+-atpase in the branchiopod artemia salina from the sebkha of sidi el hani (tunisia) |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.umontpellier.fr/hal-03411067 https://hal.umontpellier.fr/hal-03411067/document https://hal.umontpellier.fr/hal-03411067/file/S0040816619305075.pdf https://doi.org/10.1016/j.tice.2020.101340 |
long_lat |
ENVELOPE(-20.331,-20.331,63.441,63.441) ENVELOPE(-134.337,-134.337,61.333,61.333) |
geographic |
Hani Livingstone |
geographic_facet |
Hani Livingstone |
genre |
Homarus gammarus |
genre_facet |
Homarus gammarus |
op_source |
ISSN: 0040-8166 Tissue and Cell https://hal.umontpellier.fr/hal-03411067 Tissue and Cell, 2020, 63, pp.UNSP 101340. ⟨10.1016/j.tice.2020.101340⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tice.2020.101340 hal-03411067 https://hal.umontpellier.fr/hal-03411067 https://hal.umontpellier.fr/hal-03411067/document https://hal.umontpellier.fr/hal-03411067/file/S0040816619305075.pdf doi:10.1016/j.tice.2020.101340 PII: S0040-8166(19)30507-5 |
op_rights |
http://creativecommons.org/licenses/by-nc/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.tice.2020.101340 |
container_title |
Tissue and Cell |
container_volume |
63 |
container_start_page |
101340 |
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1796310655103401984 |