New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach
International audience Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France ca...
Published in: | Marine Biology |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2014
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Online Access: | https://hal.univ-brest.fr/hal-01020946 https://doi.org/10.1007/s00227-014-2470-2 |
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ftmuseumnhn:oai:HAL:hal-01020946v1 |
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openpolar |
institution |
Open Polar |
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Muséum National d'Histoire Naturelle (MNHM): HAL |
op_collection_id |
ftmuseumnhn |
language |
English |
topic |
IDENTIFICATION SUMMER MORTALITY INFECTION HALIOTIS-TUBERCULATA SURVIVAL OYSTER CRASSOSTREA-GIGAS ACL SUSCEPTIBILITY HARVEYI LINKAGE MAP IMMUNE-RESPONSE [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
IDENTIFICATION SUMMER MORTALITY INFECTION HALIOTIS-TUBERCULATA SURVIVAL OYSTER CRASSOSTREA-GIGAS ACL SUSCEPTIBILITY HARVEYI LINKAGE MAP IMMUNE-RESPONSE [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology Meistertzheim, Anne-Leila Calves, Isabelle Roussel, Véronique, V. van Wormhoudt, Alain Laroche, Jean Huchette, Sylvain Paillard, Christine New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach |
topic_facet |
IDENTIFICATION SUMMER MORTALITY INFECTION HALIOTIS-TUBERCULATA SURVIVAL OYSTER CRASSOSTREA-GIGAS ACL SUSCEPTIBILITY HARVEYI LINKAGE MAP IMMUNE-RESPONSE [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France caused by a complex interaction between the host, pathogen and environmental factors. Here, we evaluated the potential of high-resolution melting (HRM) analysis for mutation genotyping and development of genetic markers for resistance to vibriosis in the gastropod species H. tuberculata. Small amplicon assays were developed and revealed genetic polymorphism between surviving and susceptible abalone obtained after two successive infections of aquaculture families in controlled conditions. Together with specific COI haplotypes, we identified particular genotypes in nascent polypeptide-associated complex subunit alpha and ferritin genes linked to the susceptibility or resistance of abalone to vibriosis. Selection of genitors based on these genes may increase the proportion in offspring of resistant individuals of more than 76 %. Finally, HRM assays constitute a very efficient genotyping tool to validate the genetic markers on a representative number of individuals of wild populations and thus identify future resistant genitors for aquaculture or conservation purposes |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre de Formation et de Recherche sur les Environnements Méditérranéens (CEFREM) Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Scea France Haliotis ANR-08-VULN-0004,EVOLFISH,Effects of global warming and contamination on the evolutionnary response of two coastal fishes(2008) European Project: 222156,EC:FP7:SME,FP7-SME-2007-1,SUDEVAB(2008) |
format |
Article in Journal/Newspaper |
author |
Meistertzheim, Anne-Leila Calves, Isabelle Roussel, Véronique, V. van Wormhoudt, Alain Laroche, Jean Huchette, Sylvain Paillard, Christine |
author_facet |
Meistertzheim, Anne-Leila Calves, Isabelle Roussel, Véronique, V. van Wormhoudt, Alain Laroche, Jean Huchette, Sylvain Paillard, Christine |
author_sort |
Meistertzheim, Anne-Leila |
title |
New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach |
title_short |
New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach |
title_full |
New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach |
title_fullStr |
New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach |
title_full_unstemmed |
New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach |
title_sort |
new genetic markers to identify european resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.univ-brest.fr/hal-01020946 https://doi.org/10.1007/s00227-014-2470-2 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://hal.univ-brest.fr/hal-01020946 Marine Biology, 2014, 161 (8), pp.1883-1893. ⟨10.1007/s00227-014-2470-2⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-014-2470-2 info:eu-repo/grantAgreement/EC/FP7/222156/EU/Sustainable Development of European SMEs engaged in Abalone Aquaculture/SUDEVAB hal-01020946 https://hal.univ-brest.fr/hal-01020946 doi:10.1007/s00227-014-2470-2 |
op_doi |
https://doi.org/10.1007/s00227-014-2470-2 |
container_title |
Marine Biology |
container_volume |
161 |
container_issue |
8 |
container_start_page |
1883 |
op_container_end_page |
1893 |
_version_ |
1796308383245008896 |
spelling |
ftmuseumnhn:oai:HAL:hal-01020946v1 2024-04-14T08:10:44+00:00 New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach Meistertzheim, Anne-Leila Calves, Isabelle Roussel, Véronique, V. van Wormhoudt, Alain Laroche, Jean Huchette, Sylvain Paillard, Christine Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Centre de Formation et de Recherche sur les Environnements Méditérranéens (CEFREM) Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Scea France Haliotis ANR-08-VULN-0004,EVOLFISH,Effects of global warming and contamination on the evolutionnary response of two coastal fishes(2008) European Project: 222156,EC:FP7:SME,FP7-SME-2007-1,SUDEVAB(2008) 2014-08-15 https://hal.univ-brest.fr/hal-01020946 https://doi.org/10.1007/s00227-014-2470-2 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-014-2470-2 info:eu-repo/grantAgreement/EC/FP7/222156/EU/Sustainable Development of European SMEs engaged in Abalone Aquaculture/SUDEVAB hal-01020946 https://hal.univ-brest.fr/hal-01020946 doi:10.1007/s00227-014-2470-2 ISSN: 0025-3162 EISSN: 1432-1793 Marine Biology https://hal.univ-brest.fr/hal-01020946 Marine Biology, 2014, 161 (8), pp.1883-1893. ⟨10.1007/s00227-014-2470-2⟩ IDENTIFICATION SUMMER MORTALITY INFECTION HALIOTIS-TUBERCULATA SURVIVAL OYSTER CRASSOSTREA-GIGAS ACL SUSCEPTIBILITY HARVEYI LINKAGE MAP IMMUNE-RESPONSE [SDV]Life Sciences [q-bio] [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2014 ftmuseumnhn https://doi.org/10.1007/s00227-014-2470-2 2024-03-21T16:39:43Z International audience Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France caused by a complex interaction between the host, pathogen and environmental factors. Here, we evaluated the potential of high-resolution melting (HRM) analysis for mutation genotyping and development of genetic markers for resistance to vibriosis in the gastropod species H. tuberculata. Small amplicon assays were developed and revealed genetic polymorphism between surviving and susceptible abalone obtained after two successive infections of aquaculture families in controlled conditions. Together with specific COI haplotypes, we identified particular genotypes in nascent polypeptide-associated complex subunit alpha and ferritin genes linked to the susceptibility or resistance of abalone to vibriosis. Selection of genitors based on these genes may increase the proportion in offspring of resistant individuals of more than 76 %. Finally, HRM assays constitute a very efficient genotyping tool to validate the genetic markers on a representative number of individuals of wild populations and thus identify future resistant genitors for aquaculture or conservation purposes Article in Journal/Newspaper Crassostrea gigas Muséum National d'Histoire Naturelle (MNHM): HAL Marine Biology 161 8 1883 1893 |