Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species
The Southern Ocean surrounding Antarctica harbours some of the most pristine marine environments remaining, but is increasingly vulnerable to anthropogenic pressures, climate change, and invasion by non-native species. Monitoring biotic responses to cumulative impacts requires temporal and spatial b...
Published in: | Science of The Total Environment |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier B.V
2024
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Online Access: | https://doi.org/10.1016/j.scitotenv.2024.175657 https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Long-distance-Southern-Ocean-environmental-DNA-eDNA/991005695568207891 https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12166786820007891/13166786810007891 |
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ftmurdochunivall:oai:alma.61MUN_INST:11166786830007891 2024-09-15T17:46:59+00:00 Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species Nester, Georgia M. Suter, Leonie Kitchener, John A. Bunce, Michael Polanowski, Andrea M. Wasserman, Johan Deagle, Bruce 2024 pdf https://doi.org/10.1016/j.scitotenv.2024.175657 https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Long-distance-Southern-Ocean-environmental-DNA-eDNA/991005695568207891 https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12166786820007891/13166786810007891 eng eng Elsevier B.V ispartof: The Science of the total environment vol 951 doi:10.1016/j.scitotenv.2024.175657 0048-9697 1879-1026 991005695568207891 https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Long-distance-Southern-Ocean-environmental-DNA-eDNA/991005695568207891 https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12166786820007891/13166786810007891 alma:61MUN_INST/bibs/991005695568207891 Crown Copyright © 2024 Open CC BY V4.0 Antarctic Biodiversity Biomonitoring Biosecurity Continuous plankton recorder Metabarcoding text Article 2024 ftmurdochunivall https://doi.org/10.1016/j.scitotenv.2024.175657 2024-09-04T23:31:24Z The Southern Ocean surrounding Antarctica harbours some of the most pristine marine environments remaining, but is increasingly vulnerable to anthropogenic pressures, climate change, and invasion by non-native species. Monitoring biotic responses to cumulative impacts requires temporal and spatial baselines and ongoing monitoring - traditionally, this has been obtained by continuous plankton recorder (CPR) surveys. Here, we conduct one of the longest environmental DNA (eDNA) transects yet, spanning over 3000 nautical miles from Hobart (Australia) to Davis Station (Antarctica). We evaluate eDNA sampling strategies for long-term open ocean biomonitoring by comparing two water volume and filter pore size combinations: large (12 L with 20 μm) and small (2 L with 0.45 μm). Employing a broad COI metabarcoding assay, we found the large sample/pore combination was better suited to open-ocean monitoring, detecting more target DNA and rare or low abundance species. Comparisons with four simultaneously conducted CPR transects revealed that eDNA detections were more diverse than CPR, with 7 (4 unique) and 4 (1 unique) phyla detections respectively. While both methods effectively delineated biodiversity patterns across the Southern Ocean, eDNA enables surveys in the presence of sea-ice where CPR cannot be conducted. Accordingly, 16 species of concern were detected along the transect using eDNA, notably in the Antarctic region (south of 60°S). These were largely attributed to hull biofouling, a recognized pathway for marine introductions into Antarctica. Given the vulnerability of Antarctic environments to potential introductions in a warming Southern Ocean, this work underscores the importance of continued biosecurity vigilance. We advocate integrating eDNA metabarcoding with long-term CPR surveys in the Southern Ocean, emphasising the urgency of its implementation. We anticipate temporal and spatial interweaving of CPR, eDNA, and biophysical data will generate a more nuanced picture of Southern Ocean ecosystems, with ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Sea ice Southern Ocean Murdoch University Research Portal Science of The Total Environment 951 175657 |
institution |
Open Polar |
collection |
Murdoch University Research Portal |
op_collection_id |
ftmurdochunivall |
language |
English |
topic |
Antarctic Biodiversity Biomonitoring Biosecurity Continuous plankton recorder Metabarcoding |
spellingShingle |
Antarctic Biodiversity Biomonitoring Biosecurity Continuous plankton recorder Metabarcoding Nester, Georgia M. Suter, Leonie Kitchener, John A. Bunce, Michael Polanowski, Andrea M. Wasserman, Johan Deagle, Bruce Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species |
topic_facet |
Antarctic Biodiversity Biomonitoring Biosecurity Continuous plankton recorder Metabarcoding |
description |
The Southern Ocean surrounding Antarctica harbours some of the most pristine marine environments remaining, but is increasingly vulnerable to anthropogenic pressures, climate change, and invasion by non-native species. Monitoring biotic responses to cumulative impacts requires temporal and spatial baselines and ongoing monitoring - traditionally, this has been obtained by continuous plankton recorder (CPR) surveys. Here, we conduct one of the longest environmental DNA (eDNA) transects yet, spanning over 3000 nautical miles from Hobart (Australia) to Davis Station (Antarctica). We evaluate eDNA sampling strategies for long-term open ocean biomonitoring by comparing two water volume and filter pore size combinations: large (12 L with 20 μm) and small (2 L with 0.45 μm). Employing a broad COI metabarcoding assay, we found the large sample/pore combination was better suited to open-ocean monitoring, detecting more target DNA and rare or low abundance species. Comparisons with four simultaneously conducted CPR transects revealed that eDNA detections were more diverse than CPR, with 7 (4 unique) and 4 (1 unique) phyla detections respectively. While both methods effectively delineated biodiversity patterns across the Southern Ocean, eDNA enables surveys in the presence of sea-ice where CPR cannot be conducted. Accordingly, 16 species of concern were detected along the transect using eDNA, notably in the Antarctic region (south of 60°S). These were largely attributed to hull biofouling, a recognized pathway for marine introductions into Antarctica. Given the vulnerability of Antarctic environments to potential introductions in a warming Southern Ocean, this work underscores the importance of continued biosecurity vigilance. We advocate integrating eDNA metabarcoding with long-term CPR surveys in the Southern Ocean, emphasising the urgency of its implementation. We anticipate temporal and spatial interweaving of CPR, eDNA, and biophysical data will generate a more nuanced picture of Southern Ocean ecosystems, with ... |
format |
Article in Journal/Newspaper |
author |
Nester, Georgia M. Suter, Leonie Kitchener, John A. Bunce, Michael Polanowski, Andrea M. Wasserman, Johan Deagle, Bruce |
author_facet |
Nester, Georgia M. Suter, Leonie Kitchener, John A. Bunce, Michael Polanowski, Andrea M. Wasserman, Johan Deagle, Bruce |
author_sort |
Nester, Georgia M. |
title |
Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species |
title_short |
Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species |
title_full |
Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species |
title_fullStr |
Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species |
title_full_unstemmed |
Long-distance Southern Ocean environmental DNA (eDNA) transect provides insights into spatial marine biota and invasion pathways for non-native species |
title_sort |
long-distance southern ocean environmental dna (edna) transect provides insights into spatial marine biota and invasion pathways for non-native species |
publisher |
Elsevier B.V |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.scitotenv.2024.175657 https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Long-distance-Southern-Ocean-environmental-DNA-eDNA/991005695568207891 https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12166786820007891/13166786810007891 |
genre |
Antarc* Antarctic Antarctica Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Sea ice Southern Ocean |
op_relation |
ispartof: The Science of the total environment vol 951 doi:10.1016/j.scitotenv.2024.175657 0048-9697 1879-1026 991005695568207891 https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Long-distance-Southern-Ocean-environmental-DNA-eDNA/991005695568207891 https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12166786820007891/13166786810007891 alma:61MUN_INST/bibs/991005695568207891 |
op_rights |
Crown Copyright © 2024 Open CC BY V4.0 |
op_doi |
https://doi.org/10.1016/j.scitotenv.2024.175657 |
container_title |
Science of The Total Environment |
container_volume |
951 |
container_start_page |
175657 |
_version_ |
1810495454805229568 |