Effect of a honey and arginine-glutamine-hydroxymethylbutyrate mixture on the healing of colon anastomosis in rats immunosuppressed with tacrolimus

• Published in: Biotechnic & Histochemistry
• Main Authors: Akbulut, S., Dogan, Z., Baskiran, A., Elbe, Hülya, Turkoz, Y.
• Other Authors: MÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
• Format: Article in Journal/Newspaper
• Language: English
• Published: Taylor & Francis Ltd 2019
• Subjects: Anastomosis; Arginine; Bursting Pressure; Colon; Glutamine; Honey; Hydroxymethylbutyrate; Tacrolimus; Tac; sami
• Online Access: https://hdl.handle.net/20.500.12809/1036; https://doi.org/10.1080/10520295.2019.1601257

Akbulut, Sami/0000-0002-6864-7711; Doğan, Z/0000-0001-7131-2317 WOS: 000466687000001 PubMed ID: 30983411 We compared the effect of honey and a mixture of arginine-glutamine-hydroxymethylbutyrate (AGHMB) on healing of a descending colon anastomosis in rats that were immunosuppressed with tacrolimus (Tac). Sprague-Dawley rats were divided into four groups: untreated control, Tac, Tac + honey and Tac + AGHMB. Colon resection and anastomosis were performed on day 14 and re-laparotomy was performed on the day 21 of the study. Anastomotic bursting pressure, macroscopic adhesion score, weekly body weight changes, histopathological features and immunohistochemical staining of TGF-beta 1 were determined for all groups. We found no significant difference in anastomotic bursting pressure among the experimental groups. We found significant weekly increases in body weight for the Tac + honey group. We found no significant difference in the weekly body weight measurements for the Tac + AGHMB group. We found significant increases in TGF-beta 1 expression in the Tac + honey group compared to the control and Tac groups. No significant differences in inflammatory cell infiltration, fibroblast proliferation or collagen deposition were found between the Tac + honey and Tac + AGHMB groups; however, a significant difference in neovascularization between these groups was found. Neovascularization in the Tac + honey group was significantly greater than for the Tac + AGHMB group. We found that both honey and the AGHMB mixture were beneficial for anastomotic wound healing in rats that were immunosuppressed using Tac.