Dryas as a Model for Studying the Root Symbioses of the Rosaceae
The nitrogen-fixing root nodule symbiosis is restricted to four plant orders: Fabales (legumes), Fagales, Cucurbitales and Rosales (Elaeagnaceae, Rhamnaceae, and Rosaceae). Interestingly all of the Rosaceae genera confirmed to contain nodulating species (i.e., Cercocarpus, Chamaebatia, Dryas, and Pu...
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Ludwig-Maximilians-Universität München
2019
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ftmuenchenepub:oai:epub.ub.uni-muenchen.de:83796 2023-05-15T16:02:44+02:00 Dryas as a Model for Studying the Root Symbioses of the Rosaceae Billault-Penneteau, Benjamin Sandre, Aline Folgmann, Jessica Parniske, Martin Pawlowski, Katharina 2019-06-04 application/pdf https://epub.ub.uni-muenchen.de/83796/1/fpls-10-00661.pdf https://epub.ub.uni-muenchen.de/83796/ http://nbn-resolving.de/urn:nbn:de:bvb:19-epub-83796-0 https://doi.org/10.3389/fpls.2019.00661 eng eng Ludwig-Maximilians-Universität München Billault-Penneteau, Benjamin; Sandre, Aline; Folgmann, Jessica; Parniske, Martin; Pawlowski, Katharina (4. Juni 2019): Dryas as a Model for Studying the Root Symbioses of the Rosaceae. In: Frontiers in Plant Science, Vol. 10, 661: S. 1-13 [PDF, 6MB] https://epub.ub.uni-muenchen.de/83796/1/fpls-10-00661.pdf http://nbn-resolving.de/urn:nbn:de:bvb:19-epub-83796-0 https://epub.ub.uni-muenchen.de/83796/ doi:10.3389/fpls.2019.00661 Frontiers in Plant Science Department Biologie I ddc:570 doc-type:article Zeitschriftenartikel PeerReviewed 2019 ftmuenchenepub https://doi.org/10.3389/fpls.2019.00661 2022-05-01T23:24:36Z The nitrogen-fixing root nodule symbiosis is restricted to four plant orders: Fabales (legumes), Fagales, Cucurbitales and Rosales (Elaeagnaceae, Rhamnaceae, and Rosaceae). Interestingly all of the Rosaceae genera confirmed to contain nodulating species (i.e., Cercocarpus, Chamaebatia, Dryas, and Purshia) belong to a single subfamily, the Dryadoideae. The Dryas genus is particularly interesting from an evolutionary perspective because it contains closely related nodulating (Dryas drummondii) and non-nodulating species (Dryas octopetala). The close phylogenetic relationship between these two species makes Dryas an ideal model genus to study the genetic basis of nodulation by whole genome comparison and classical genetics. Therefore, we established methods for plant cultivation, transformation and DNA extraction for these species. We optimized seed surface sterilization and germination methods and tested growth protocols ranging from pots and Petri dishes to a hydroponic system. Transgenic hairy roots were obtained by adapting Agrobacterium rhizogenes-based transformation protocols for Dryas species. We compared several DNA extraction protocols for their suitability for subsequent molecular biological analysis. Using CTAB extraction, reproducible PCRs could be performed, but CsCl gradient purification was essential to obtain DNA in sufficient purity for high quality de novo genome sequencing of both Dryas species. Altogether, we established a basic toolkit for the culture, transient transformation and genetic analysis of Dryas sp. Article in Journal/Newspaper Dryas octopetala Open Access LMU (Ludwig-Maximilians-University Munich) Rosales ENVELOPE(-59.867,-59.867,-62.600,-62.600) Frontiers in Plant Science 10 |
institution |
Open Polar |
collection |
Open Access LMU (Ludwig-Maximilians-University Munich) |
op_collection_id |
ftmuenchenepub |
language |
English |
topic |
Department Biologie I ddc:570 |
spellingShingle |
Department Biologie I ddc:570 Billault-Penneteau, Benjamin Sandre, Aline Folgmann, Jessica Parniske, Martin Pawlowski, Katharina Dryas as a Model for Studying the Root Symbioses of the Rosaceae |
topic_facet |
Department Biologie I ddc:570 |
description |
The nitrogen-fixing root nodule symbiosis is restricted to four plant orders: Fabales (legumes), Fagales, Cucurbitales and Rosales (Elaeagnaceae, Rhamnaceae, and Rosaceae). Interestingly all of the Rosaceae genera confirmed to contain nodulating species (i.e., Cercocarpus, Chamaebatia, Dryas, and Purshia) belong to a single subfamily, the Dryadoideae. The Dryas genus is particularly interesting from an evolutionary perspective because it contains closely related nodulating (Dryas drummondii) and non-nodulating species (Dryas octopetala). The close phylogenetic relationship between these two species makes Dryas an ideal model genus to study the genetic basis of nodulation by whole genome comparison and classical genetics. Therefore, we established methods for plant cultivation, transformation and DNA extraction for these species. We optimized seed surface sterilization and germination methods and tested growth protocols ranging from pots and Petri dishes to a hydroponic system. Transgenic hairy roots were obtained by adapting Agrobacterium rhizogenes-based transformation protocols for Dryas species. We compared several DNA extraction protocols for their suitability for subsequent molecular biological analysis. Using CTAB extraction, reproducible PCRs could be performed, but CsCl gradient purification was essential to obtain DNA in sufficient purity for high quality de novo genome sequencing of both Dryas species. Altogether, we established a basic toolkit for the culture, transient transformation and genetic analysis of Dryas sp. |
format |
Article in Journal/Newspaper |
author |
Billault-Penneteau, Benjamin Sandre, Aline Folgmann, Jessica Parniske, Martin Pawlowski, Katharina |
author_facet |
Billault-Penneteau, Benjamin Sandre, Aline Folgmann, Jessica Parniske, Martin Pawlowski, Katharina |
author_sort |
Billault-Penneteau, Benjamin |
title |
Dryas as a Model for Studying the Root Symbioses of the Rosaceae |
title_short |
Dryas as a Model for Studying the Root Symbioses of the Rosaceae |
title_full |
Dryas as a Model for Studying the Root Symbioses of the Rosaceae |
title_fullStr |
Dryas as a Model for Studying the Root Symbioses of the Rosaceae |
title_full_unstemmed |
Dryas as a Model for Studying the Root Symbioses of the Rosaceae |
title_sort |
dryas as a model for studying the root symbioses of the rosaceae |
publisher |
Ludwig-Maximilians-Universität München |
publishDate |
2019 |
url |
https://epub.ub.uni-muenchen.de/83796/1/fpls-10-00661.pdf https://epub.ub.uni-muenchen.de/83796/ http://nbn-resolving.de/urn:nbn:de:bvb:19-epub-83796-0 https://doi.org/10.3389/fpls.2019.00661 |
long_lat |
ENVELOPE(-59.867,-59.867,-62.600,-62.600) |
geographic |
Rosales |
geographic_facet |
Rosales |
genre |
Dryas octopetala |
genre_facet |
Dryas octopetala |
op_source |
Frontiers in Plant Science |
op_relation |
Billault-Penneteau, Benjamin; Sandre, Aline; Folgmann, Jessica; Parniske, Martin; Pawlowski, Katharina (4. Juni 2019): Dryas as a Model for Studying the Root Symbioses of the Rosaceae. In: Frontiers in Plant Science, Vol. 10, 661: S. 1-13 [PDF, 6MB] https://epub.ub.uni-muenchen.de/83796/1/fpls-10-00661.pdf http://nbn-resolving.de/urn:nbn:de:bvb:19-epub-83796-0 https://epub.ub.uni-muenchen.de/83796/ doi:10.3389/fpls.2019.00661 |
op_doi |
https://doi.org/10.3389/fpls.2019.00661 |
container_title |
Frontiers in Plant Science |
container_volume |
10 |
_version_ |
1766398420434550784 |