Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol
Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris–sucrose–KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris–NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris–sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented wit...
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ftmtak:oai:real.mtak.hu:5070 2024-06-09T07:49:31+00:00 Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol Glogowski, J. Kolman, R. Szczepkowski, M. Horváth, Ákos Urbányi, Béla Sieczyński, P. Rzemieniecki, A. Domagała, J. Demianowicz, W. Kowalski, R. Ciereszko, A. 2002 text https://real.mtak.hu/5070/ https://real.mtak.hu/5070/1/1134201.pdf https://doi.org/10.1016/S0044-8486(02)00003-0 en eng Elsevier https://real.mtak.hu/5070/1/1134201.pdf Glogowski, J. and Kolman, R. and Szczepkowski, M. and Horváth, Ákos and Urbányi, Béla and Sieczyński, P. and Rzemieniecki, A. and Domagała, J. and Demianowicz, W. and Kowalski, R. and Ciereszko, A. (2002) Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture, 211 (1-4). pp. 367-373. ISSN 0044-8486 SF Animal culture / állattenyésztés SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás halászat sporthorgászat Article PeerReviewed info:eu-repo/semantics/article 2002 ftmtak https://doi.org/10.1016/S0044-8486(02)00003-0 2024-05-15T13:44:04Z Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris–sucrose–KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris–NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris–sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris–sucrose–KCl, Tris–NaCl, and Tris–sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris–sucrose–KCl and Tris–NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen. Article in Journal/Newspaper Siberian sturgeon MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences Aquaculture 211 1-4 367 373 |
institution |
Open Polar |
collection |
MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences |
op_collection_id |
ftmtak |
language |
English |
topic |
SF Animal culture / állattenyésztés SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás halászat sporthorgászat |
spellingShingle |
SF Animal culture / állattenyésztés SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás halászat sporthorgászat Glogowski, J. Kolman, R. Szczepkowski, M. Horváth, Ákos Urbányi, Béla Sieczyński, P. Rzemieniecki, A. Domagała, J. Demianowicz, W. Kowalski, R. Ciereszko, A. Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol |
topic_facet |
SF Animal culture / állattenyésztés SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás halászat sporthorgászat |
description |
Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris–sucrose–KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris–NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris–sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris–sucrose–KCl, Tris–NaCl, and Tris–sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris–sucrose–KCl and Tris–NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen. |
format |
Article in Journal/Newspaper |
author |
Glogowski, J. Kolman, R. Szczepkowski, M. Horváth, Ákos Urbányi, Béla Sieczyński, P. Rzemieniecki, A. Domagała, J. Demianowicz, W. Kowalski, R. Ciereszko, A. |
author_facet |
Glogowski, J. Kolman, R. Szczepkowski, M. Horváth, Ákos Urbányi, Béla Sieczyński, P. Rzemieniecki, A. Domagała, J. Demianowicz, W. Kowalski, R. Ciereszko, A. |
author_sort |
Glogowski, J. |
title |
Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol |
title_short |
Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol |
title_full |
Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol |
title_fullStr |
Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol |
title_full_unstemmed |
Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol |
title_sort |
fertilization rate of siberian sturgeon (acipenser baeri, brandt) milt cryopreserved with methanol |
publisher |
Elsevier |
publishDate |
2002 |
url |
https://real.mtak.hu/5070/ https://real.mtak.hu/5070/1/1134201.pdf https://doi.org/10.1016/S0044-8486(02)00003-0 |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_relation |
https://real.mtak.hu/5070/1/1134201.pdf Glogowski, J. and Kolman, R. and Szczepkowski, M. and Horváth, Ákos and Urbányi, Béla and Sieczyński, P. and Rzemieniecki, A. and Domagała, J. and Demianowicz, W. and Kowalski, R. and Ciereszko, A. (2002) Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture, 211 (1-4). pp. 367-373. ISSN 0044-8486 |
op_doi |
https://doi.org/10.1016/S0044-8486(02)00003-0 |
container_title |
Aquaculture |
container_volume |
211 |
container_issue |
1-4 |
container_start_page |
367 |
op_container_end_page |
373 |
_version_ |
1801382166320381952 |