Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris–sucrose–KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris–NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris–sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented wit...

Full description

Bibliographic Details
Published in:Aquaculture
Main Authors: Glogowski, J., Kolman, R., Szczepkowski, M., Horváth, Ákos, Urbányi, Béla, Sieczyński, P., Rzemieniecki, A., Domagała, J., Demianowicz, W., Kowalski, R., Ciereszko, A.
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2002
Subjects:
SF Animal culture / állattenyésztés
SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás
halászat
sporthorgászat
Siberian sturgeon
Online Access:https://real.mtak.hu/5070/
https://real.mtak.hu/5070/1/1134201.pdf
https://doi.org/10.1016/S0044-8486(02)00003-0
Description
Summary:Milt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris–sucrose–KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris–NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris–sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris–sucrose–KCl, Tris–NaCl, and Tris–sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris–sucrose–KCl and Tris–NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen.

Similar Items