Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases
Electrospinning was applied to create easy-to-handle and high- surface-area membranes from continuous nanofibers of polyvinyl alcohol (PVA) or polylactic acid (PLA). Lipase PS from Burkholderia cepacia and Lipase B from Candida antarctica (CaLB) could be immobilized effectively by adsorption onto th...
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ftmtak:oai:real.mtak.hu:40137 2023-05-15T13:56:11+02:00 Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases Sóti, Péter Lajos Weiser, Diána Vigh, Tamás Nagy, Zsombor Kristóf Poppe, László Marosi, György 2016 text http://real.mtak.hu/40137/ http://real.mtak.hu/40137/1/Soti_Electrospun_polylactic_acid_and_polyvinyl_alcohol_fibers_as_efficient_and_stable_nanomaterials_for_immobilization_of_lipases10.1007s00449_015_1528_y_u.pdf https://doi.org/10.1007/s00449-015-1528-y en eng Springer http://real.mtak.hu/40137/1/Soti_Electrospun_polylactic_acid_and_polyvinyl_alcohol_fibers_as_efficient_and_stable_nanomaterials_for_immobilization_of_lipases10.1007s00449_015_1528_y_u.pdf Sóti, Péter Lajos and Weiser, Diána and Vigh, Tamás and Nagy, Zsombor Kristóf and Poppe, László and Marosi, György (2016) Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 39 (3). pp. 449-459. ISSN 1615-7591 QD Chemistry / kémia Article PeerReviewed 2016 ftmtak https://doi.org/10.1007/s00449-015-1528-y 2017-09-20T22:55:13Z Electrospinning was applied to create easy-to-handle and high- surface-area membranes from continuous nanofibers of polyvinyl alcohol (PVA) or polylactic acid (PLA). Lipase PS from Burkholderia cepacia and Lipase B from Candida antarctica (CaLB) could be immobilized effectively by adsorption onto the fibrous material as well as by entrapment within the electrospun nanofibers. The biocatalytic performance of the resulting membrane biocatalysts was evaluated in the kinetic resolution of racemic 1-phenylethanol (rac-1) and 1-phenylethyl acetate (rac- 2). Fine dispersion of the enzymes in the polymer matrix and large surface area of the nanofibers resulted in an enormous increase in the activity of the membrane biocatalyst compared to the non-immobilized crude powder forms of the lipases. PLA as fiber-forming polymer for lipase immobilization performed better than PVA in all aspects. Recycling studies with the various forms of electrospun membrane biocatalysts in ten cycles of the acylation and hydrolysis reactions indicated excellent stability of this forms of immobilized lipases. PLA-entrapped lipases could preserve lipase activity and enantiomer selectivity much better than the PVA-entrapped forms. The electrospun membrane forms of CaLB showed high mechanical stability in the repeated acylations and hydrolyses than commercial forms of CaLB immobilized on polyacrylamide beads (Novozyme 435 and IMMCALB- T2-150). © 2016 Springer-Verlag Berlin Heidelberg Article in Journal/Newspaper Antarc* Antarctica MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences Bioprocess and Biosystems Engineering 39 3 449 459 |
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MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences |
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ftmtak |
language |
English |
topic |
QD Chemistry / kémia |
spellingShingle |
QD Chemistry / kémia Sóti, Péter Lajos Weiser, Diána Vigh, Tamás Nagy, Zsombor Kristóf Poppe, László Marosi, György Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases |
topic_facet |
QD Chemistry / kémia |
description |
Electrospinning was applied to create easy-to-handle and high- surface-area membranes from continuous nanofibers of polyvinyl alcohol (PVA) or polylactic acid (PLA). Lipase PS from Burkholderia cepacia and Lipase B from Candida antarctica (CaLB) could be immobilized effectively by adsorption onto the fibrous material as well as by entrapment within the electrospun nanofibers. The biocatalytic performance of the resulting membrane biocatalysts was evaluated in the kinetic resolution of racemic 1-phenylethanol (rac-1) and 1-phenylethyl acetate (rac- 2). Fine dispersion of the enzymes in the polymer matrix and large surface area of the nanofibers resulted in an enormous increase in the activity of the membrane biocatalyst compared to the non-immobilized crude powder forms of the lipases. PLA as fiber-forming polymer for lipase immobilization performed better than PVA in all aspects. Recycling studies with the various forms of electrospun membrane biocatalysts in ten cycles of the acylation and hydrolysis reactions indicated excellent stability of this forms of immobilized lipases. PLA-entrapped lipases could preserve lipase activity and enantiomer selectivity much better than the PVA-entrapped forms. The electrospun membrane forms of CaLB showed high mechanical stability in the repeated acylations and hydrolyses than commercial forms of CaLB immobilized on polyacrylamide beads (Novozyme 435 and IMMCALB- T2-150). © 2016 Springer-Verlag Berlin Heidelberg |
format |
Article in Journal/Newspaper |
author |
Sóti, Péter Lajos Weiser, Diána Vigh, Tamás Nagy, Zsombor Kristóf Poppe, László Marosi, György |
author_facet |
Sóti, Péter Lajos Weiser, Diána Vigh, Tamás Nagy, Zsombor Kristóf Poppe, László Marosi, György |
author_sort |
Sóti, Péter Lajos |
title |
Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases |
title_short |
Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases |
title_full |
Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases |
title_fullStr |
Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases |
title_full_unstemmed |
Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases |
title_sort |
electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases |
publisher |
Springer |
publishDate |
2016 |
url |
http://real.mtak.hu/40137/ http://real.mtak.hu/40137/1/Soti_Electrospun_polylactic_acid_and_polyvinyl_alcohol_fibers_as_efficient_and_stable_nanomaterials_for_immobilization_of_lipases10.1007s00449_015_1528_y_u.pdf https://doi.org/10.1007/s00449-015-1528-y |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://real.mtak.hu/40137/1/Soti_Electrospun_polylactic_acid_and_polyvinyl_alcohol_fibers_as_efficient_and_stable_nanomaterials_for_immobilization_of_lipases10.1007s00449_015_1528_y_u.pdf Sóti, Péter Lajos and Weiser, Diána and Vigh, Tamás and Nagy, Zsombor Kristóf and Poppe, László and Marosi, György (2016) Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 39 (3). pp. 449-459. ISSN 1615-7591 |
op_doi |
https://doi.org/10.1007/s00449-015-1528-y |
container_title |
Bioprocess and Biosystems Engineering |
container_volume |
39 |
container_issue |
3 |
container_start_page |
449 |
op_container_end_page |
459 |
_version_ |
1766263542489546752 |