Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts
Immobilization of lipases from Pseudomonas fluorescens (Lipase AK), Burkholderia (Pseudomonas) cepacia (Lipase PS) and lipase B from Pseudozyma (Candida) antarctica (CaLB) was investigated by entrapment in electrospun poly(vinyl alcohol) (PVA) nanofibers. The activity and selectivity of the lipases...
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ftmtak:oai:real.mtak.hu:40135 2023-05-15T13:56:24+02:00 Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts Weiser, Diána Sóti, Péter Lajos Bánóczi, Gergely Bódai, Viktória Kiss, Bálint Gellért, Ákos Nagy, Zsombor Kristóf Koczka, Béla Szilágyi, András Ferenc Marosi, György Poppe, László 2016 text http://real.mtak.hu/40135/ http://real.mtak.hu/40135/1/Weiser_2016_Bioimprinted_lipases_in_PVA_nanofibers_as_efficient_immobilized_biocatalysts_u.pdf https://doi.org/10.1016/j.tet.2016.06.027 en eng http://real.mtak.hu/40135/1/Weiser_2016_Bioimprinted_lipases_in_PVA_nanofibers_as_efficient_immobilized_biocatalysts_u.pdf Weiser, Diána and Sóti, Péter Lajos and Bánóczi, Gergely and Bódai, Viktória and Kiss, Bálint and Gellért, Ákos and Nagy, Zsombor Kristóf and Koczka, Béla and Szilágyi, András Ferenc and Marosi, György and Poppe, László (2016) Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts. TETRAHEDRON. ISSN 0040-4020 (In Press) QD Chemistry / kémia Article PeerReviewed 2016 ftmtak https://doi.org/10.1016/j.tet.2016.06.027 2018-01-03T23:59:39Z Immobilization of lipases from Pseudomonas fluorescens (Lipase AK), Burkholderia (Pseudomonas) cepacia (Lipase PS) and lipase B from Pseudozyma (Candida) antarctica (CaLB) was investigated by entrapment in electrospun poly(vinyl alcohol) (PVA) nanofibers. The activity and selectivity of the lipases entrapped in PVA nanofibers were characterized in kinetic resolution of racemic secondary alcohols using acylation in organic media. Potential bioimprinting effect of eight substrate mimicking additives [polyethylene glycols (PEGs), non-ionic detergents (NIDs) and various organosilanes] was tested with the fiber-entrapped lipases. The nanofibrous lipase biocatalyst entrapped in the presence of the additives were also characterized by rheology, differential scanning calorimetry and scanning electron microscopy. In addition to the known lipase-bioimprinting agents (PEGs, NIDs), phenyl- and octyltriethoxysilane also enhanced substantially the biocatalytic properties of lipases in their electrospun PVA fiber-entrapped forms. The reasons of bioimprinting effect of several additives were rationalized by docking studies in the open and closed form of CaLB. Article in Journal/Newspaper Antarc* Antarctica MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences Tetrahedron 72 46 7335 7342 |
institution |
Open Polar |
collection |
MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences |
op_collection_id |
ftmtak |
language |
English |
topic |
QD Chemistry / kémia |
spellingShingle |
QD Chemistry / kémia Weiser, Diána Sóti, Péter Lajos Bánóczi, Gergely Bódai, Viktória Kiss, Bálint Gellért, Ákos Nagy, Zsombor Kristóf Koczka, Béla Szilágyi, András Ferenc Marosi, György Poppe, László Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts |
topic_facet |
QD Chemistry / kémia |
description |
Immobilization of lipases from Pseudomonas fluorescens (Lipase AK), Burkholderia (Pseudomonas) cepacia (Lipase PS) and lipase B from Pseudozyma (Candida) antarctica (CaLB) was investigated by entrapment in electrospun poly(vinyl alcohol) (PVA) nanofibers. The activity and selectivity of the lipases entrapped in PVA nanofibers were characterized in kinetic resolution of racemic secondary alcohols using acylation in organic media. Potential bioimprinting effect of eight substrate mimicking additives [polyethylene glycols (PEGs), non-ionic detergents (NIDs) and various organosilanes] was tested with the fiber-entrapped lipases. The nanofibrous lipase biocatalyst entrapped in the presence of the additives were also characterized by rheology, differential scanning calorimetry and scanning electron microscopy. In addition to the known lipase-bioimprinting agents (PEGs, NIDs), phenyl- and octyltriethoxysilane also enhanced substantially the biocatalytic properties of lipases in their electrospun PVA fiber-entrapped forms. The reasons of bioimprinting effect of several additives were rationalized by docking studies in the open and closed form of CaLB. |
format |
Article in Journal/Newspaper |
author |
Weiser, Diána Sóti, Péter Lajos Bánóczi, Gergely Bódai, Viktória Kiss, Bálint Gellért, Ákos Nagy, Zsombor Kristóf Koczka, Béla Szilágyi, András Ferenc Marosi, György Poppe, László |
author_facet |
Weiser, Diána Sóti, Péter Lajos Bánóczi, Gergely Bódai, Viktória Kiss, Bálint Gellért, Ákos Nagy, Zsombor Kristóf Koczka, Béla Szilágyi, András Ferenc Marosi, György Poppe, László |
author_sort |
Weiser, Diána |
title |
Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts |
title_short |
Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts |
title_full |
Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts |
title_fullStr |
Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts |
title_full_unstemmed |
Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts |
title_sort |
bioimprinted lipases in pva nanofibers as efficient immobilized biocatalysts |
publishDate |
2016 |
url |
http://real.mtak.hu/40135/ http://real.mtak.hu/40135/1/Weiser_2016_Bioimprinted_lipases_in_PVA_nanofibers_as_efficient_immobilized_biocatalysts_u.pdf https://doi.org/10.1016/j.tet.2016.06.027 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://real.mtak.hu/40135/1/Weiser_2016_Bioimprinted_lipases_in_PVA_nanofibers_as_efficient_immobilized_biocatalysts_u.pdf Weiser, Diána and Sóti, Péter Lajos and Bánóczi, Gergely and Bódai, Viktória and Kiss, Bálint and Gellért, Ákos and Nagy, Zsombor Kristóf and Koczka, Béla and Szilágyi, András Ferenc and Marosi, György and Poppe, László (2016) Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts. TETRAHEDRON. ISSN 0040-4020 (In Press) |
op_doi |
https://doi.org/10.1016/j.tet.2016.06.027 |
container_title |
Tetrahedron |
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72 |
container_issue |
46 |
container_start_page |
7335 |
op_container_end_page |
7342 |
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1766263906835103744 |