Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations
Glycerol diglycidyl ether (GDE) is a convenient and inexpensive bis-epoxide cross-linker as demonstrated by the preparation of cross-linked enzyme aggregates (CLEAs) from two different enzyme classes. GDE cross-linked CLEAs of lipases from Pseudomonas fluorescens (AK), Burkholderia cepacia (PS) and...
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ftmtak:oai:real.mtak.hu:19300 2024-06-09T07:38:49+00:00 Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations Weiser, Diána Varga, A. Kovács, K. Nagy, F. Szilágyi, András Ferenc Vértessy G., Beáta Paizs, Cs. Poppe, László 2014 text https://real.mtak.hu/19300/ https://real.mtak.hu/19300/1/Weiser_ChemCatChem_2014.pdf http://onlinelibrary.wiley.com/doi/10.1002/cctc.201300806/abstract en eng Wiley https://real.mtak.hu/19300/1/Weiser_ChemCatChem_2014.pdf Weiser, Diána and Varga, A. and Kovács, K. and Nagy, F. and Szilágyi, András Ferenc and Vértessy G., Beáta and Paizs, Cs. and Poppe, László (2014) Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations. CHEMCATCHEM, 6 (5). pp. 1463-1469. ISSN 1867-3880 QD Chemistry / kémia Article PeerReviewed info:eu-repo/semantics/article 2014 ftmtak 2024-05-15T13:44:05Z Glycerol diglycidyl ether (GDE) is a convenient and inexpensive bis-epoxide cross-linker as demonstrated by the preparation of cross-linked enzyme aggregates (CLEAs) from two different enzyme classes. GDE cross-linked CLEAs of lipases from Pseudomonas fluorescens (AK), Burkholderia cepacia (PS) and lipase B from Candida antarctica (CaL B) and further of phenylalanine ammonia-lyase (PAL) from Petroselinum crispum showed improved properties as compared to their glutaraldehyde (GA) cross-linked counterparts. Ultrasonication studies indicated GDE cross-linked CLEAs of lipase PS and PAL as mechanically more stable than the GA-based forms. In the kinetic resolution of racemic 1-phenylethanol 1 catalytic activity of GDE-based lipase CLEAs (U= 69.6, 134.8 and 127.4 U g-1; for AK, CaL B and PS prepared at 22 °C, respectively) surpassed those of the corresponding GA-based lipase CLEAs (U= 24.4, 131.0 and 119.2 U g-1; for AK, CaL B and PS prepared at 22 °C, respectively). GDE-based PAL-bovine serum albumin co-CLEAs could be recycled at least three times when used for the stereoselective ammonia addition in 6M ammonia onto (E)-3-(thiophen-2-yl)acrylic acid 4 whereas recycling of conventional GA-based PAL CLEAs from this medium failed. Article in Journal/Newspaper Antarc* Antarctica MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences |
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MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences |
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ftmtak |
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English |
topic |
QD Chemistry / kémia |
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QD Chemistry / kémia Weiser, Diána Varga, A. Kovács, K. Nagy, F. Szilágyi, András Ferenc Vértessy G., Beáta Paizs, Cs. Poppe, László Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations |
topic_facet |
QD Chemistry / kémia |
description |
Glycerol diglycidyl ether (GDE) is a convenient and inexpensive bis-epoxide cross-linker as demonstrated by the preparation of cross-linked enzyme aggregates (CLEAs) from two different enzyme classes. GDE cross-linked CLEAs of lipases from Pseudomonas fluorescens (AK), Burkholderia cepacia (PS) and lipase B from Candida antarctica (CaL B) and further of phenylalanine ammonia-lyase (PAL) from Petroselinum crispum showed improved properties as compared to their glutaraldehyde (GA) cross-linked counterparts. Ultrasonication studies indicated GDE cross-linked CLEAs of lipase PS and PAL as mechanically more stable than the GA-based forms. In the kinetic resolution of racemic 1-phenylethanol 1 catalytic activity of GDE-based lipase CLEAs (U= 69.6, 134.8 and 127.4 U g-1; for AK, CaL B and PS prepared at 22 °C, respectively) surpassed those of the corresponding GA-based lipase CLEAs (U= 24.4, 131.0 and 119.2 U g-1; for AK, CaL B and PS prepared at 22 °C, respectively). GDE-based PAL-bovine serum albumin co-CLEAs could be recycled at least three times when used for the stereoselective ammonia addition in 6M ammonia onto (E)-3-(thiophen-2-yl)acrylic acid 4 whereas recycling of conventional GA-based PAL CLEAs from this medium failed. |
format |
Article in Journal/Newspaper |
author |
Weiser, Diána Varga, A. Kovács, K. Nagy, F. Szilágyi, András Ferenc Vértessy G., Beáta Paizs, Cs. Poppe, László |
author_facet |
Weiser, Diána Varga, A. Kovács, K. Nagy, F. Szilágyi, András Ferenc Vértessy G., Beáta Paizs, Cs. Poppe, László |
author_sort |
Weiser, Diána |
title |
Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations |
title_short |
Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations |
title_full |
Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations |
title_fullStr |
Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations |
title_full_unstemmed |
Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations |
title_sort |
bisepoxide cross-linked enzyme aggregates – new immobilized biocatalysts for selective biotransformations |
publisher |
Wiley |
publishDate |
2014 |
url |
https://real.mtak.hu/19300/ https://real.mtak.hu/19300/1/Weiser_ChemCatChem_2014.pdf http://onlinelibrary.wiley.com/doi/10.1002/cctc.201300806/abstract |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://real.mtak.hu/19300/1/Weiser_ChemCatChem_2014.pdf Weiser, Diána and Varga, A. and Kovács, K. and Nagy, F. and Szilágyi, András Ferenc and Vértessy G., Beáta and Paizs, Cs. and Poppe, László (2014) Bisepoxide Cross-Linked Enzyme Aggregates – New Immobilized Biocatalysts for Selective Biotransformations. CHEMCATCHEM, 6 (5). pp. 1463-1469. ISSN 1867-3880 |
_version_ |
1801375553021804544 |