Immobilization engineering – How to design advanced sol-gel systems for biocatalysis?
An immobilization engineering approach using bioinformatics and experimental design tools was applied to improve the sol–gel enzyme entrapment methodology. This strategy was used for the immobilization of lipase B from Candida antarctica (CaLB), a versatile enzyme widely used even on the industrial...
Published in: | Green Chemistry |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Royal Society of Chemistry
2017
|
Subjects: | |
Online Access: | http://real.mtak.hu/101990/ http://real.mtak.hu/101990/2/c7gc00.pdf https://doi.org/10.1039/c7gc00896a |
id |
ftmtak:oai:real.mtak.hu:101990 |
---|---|
record_format |
openpolar |
spelling |
ftmtak:oai:real.mtak.hu:101990 2023-06-11T04:04:09+02:00 Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? Balogh Weiser, Diána Nagy, Flóra Bánóczi, Gergely Oláh, Márk Farkas, Attila Szilágyi, András Ferenc Nagyné László, Krisztina Gellért, Ákos Marosi, György Kemény, Sándor Poppe, László 2017 text http://real.mtak.hu/101990/ http://real.mtak.hu/101990/2/c7gc00.pdf https://doi.org/10.1039/c7gc00896a en eng Royal Society of Chemistry http://real.mtak.hu/101990/2/c7gc00.pdf Balogh Weiser, Diána and Nagy, Flóra and Bánóczi, Gergely and Oláh, Márk and Farkas, Attila and Szilágyi, András Ferenc and Nagyné László, Krisztina and Gellért, Ákos and Marosi, György and Kemény, Sándor and Poppe, László (2017) Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? Green Chemistry, 19 (16). pp. 3927-3937. ISSN 1463-9262, ESSN: 1463-9270 QD Chemistry / kémia Article PeerReviewed info:eu-repo/semantics/article 2017 ftmtak https://doi.org/10.1039/c7gc00896a 2023-04-19T23:29:10Z An immobilization engineering approach using bioinformatics and experimental design tools was applied to improve the sol–gel enzyme entrapment methodology. This strategy was used for the immobilization of lipase B from Candida antarctica (CaLB), a versatile enzyme widely used even on the industrial scale. The optimized entrapment of CaLB in sol–gel matrices is reported by the response-surface methodology enabling efficient process development. The immobilized CaLBs characterized by functional efficiency and enhanced recovery provided economical and green options for flow chemistry. Various ternary mixtures of sol–gel precursors allowed the creation of tailored entrapment matrices best suited for the enzyme and its targeted substrate. The sol–gel-entrapped forms of CaLB were excellent biocatalysts in the kinetic resolutions of secondary alcohols and secondary amines with aromatic or aliphatic substituents both in batch and continuous-flow biotransformations. Article in Journal/Newspaper Antarc* Antarctica MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences Green Chemistry 19 16 3927 3937 |
institution |
Open Polar |
collection |
MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences |
op_collection_id |
ftmtak |
language |
English |
topic |
QD Chemistry / kémia |
spellingShingle |
QD Chemistry / kémia Balogh Weiser, Diána Nagy, Flóra Bánóczi, Gergely Oláh, Márk Farkas, Attila Szilágyi, András Ferenc Nagyné László, Krisztina Gellért, Ákos Marosi, György Kemény, Sándor Poppe, László Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? |
topic_facet |
QD Chemistry / kémia |
description |
An immobilization engineering approach using bioinformatics and experimental design tools was applied to improve the sol–gel enzyme entrapment methodology. This strategy was used for the immobilization of lipase B from Candida antarctica (CaLB), a versatile enzyme widely used even on the industrial scale. The optimized entrapment of CaLB in sol–gel matrices is reported by the response-surface methodology enabling efficient process development. The immobilized CaLBs characterized by functional efficiency and enhanced recovery provided economical and green options for flow chemistry. Various ternary mixtures of sol–gel precursors allowed the creation of tailored entrapment matrices best suited for the enzyme and its targeted substrate. The sol–gel-entrapped forms of CaLB were excellent biocatalysts in the kinetic resolutions of secondary alcohols and secondary amines with aromatic or aliphatic substituents both in batch and continuous-flow biotransformations. |
format |
Article in Journal/Newspaper |
author |
Balogh Weiser, Diána Nagy, Flóra Bánóczi, Gergely Oláh, Márk Farkas, Attila Szilágyi, András Ferenc Nagyné László, Krisztina Gellért, Ákos Marosi, György Kemény, Sándor Poppe, László |
author_facet |
Balogh Weiser, Diána Nagy, Flóra Bánóczi, Gergely Oláh, Márk Farkas, Attila Szilágyi, András Ferenc Nagyné László, Krisztina Gellért, Ákos Marosi, György Kemény, Sándor Poppe, László |
author_sort |
Balogh Weiser, Diána |
title |
Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? |
title_short |
Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? |
title_full |
Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? |
title_fullStr |
Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? |
title_full_unstemmed |
Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? |
title_sort |
immobilization engineering – how to design advanced sol-gel systems for biocatalysis? |
publisher |
Royal Society of Chemistry |
publishDate |
2017 |
url |
http://real.mtak.hu/101990/ http://real.mtak.hu/101990/2/c7gc00.pdf https://doi.org/10.1039/c7gc00896a |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://real.mtak.hu/101990/2/c7gc00.pdf Balogh Weiser, Diána and Nagy, Flóra and Bánóczi, Gergely and Oláh, Márk and Farkas, Attila and Szilágyi, András Ferenc and Nagyné László, Krisztina and Gellért, Ákos and Marosi, György and Kemény, Sándor and Poppe, László (2017) Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? Green Chemistry, 19 (16). pp. 3927-3937. ISSN 1463-9262, ESSN: 1463-9270 |
op_doi |
https://doi.org/10.1039/c7gc00896a |
container_title |
Green Chemistry |
container_volume |
19 |
container_issue |
16 |
container_start_page |
3927 |
op_container_end_page |
3937 |
_version_ |
1768385760278347776 |