Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon
2013 Fall. Includes bibliographical references. Aquaculture is a rapidly growing industry that significantly contributes to the world food supply. Sustainable practices in aquaculture are of increasing importance as an increasing proportion of fish in the global market come from aquaculture instead...
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ftmountainschol:oai:mountainscholar.org:10217/81048 2023-06-11T04:10:13+02:00 Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon Orahood, Darcy Sonya Salman, M. D. Eckery, Douglas C. Miller, Lowell A. Myrick, Christopher A. Rhyan, Jack C. 2007-01-03T06:11:32Z born digital masters theses application/pdf http://hdl.handle.net/10217/81048 English eng eng Colorado State University. Libraries 2000-2019 - CSU Theses and Dissertations Orahood_colostate_0053N_12117.pdf ETDF2013500404CLIS http://hdl.handle.net/10217/81048 Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. vaccine reproduction immunocontraception Atlantic salmon Text 2007 ftmountainschol 2023-05-06T17:46:56Z 2013 Fall. Includes bibliographical references. Aquaculture is a rapidly growing industry that significantly contributes to the world food supply. Sustainable practices in aquaculture are of increasing importance as an increasing proportion of fish in the global market come from aquaculture instead of wild catch. Maximizing aquaculture yields and minimizing the ecological impacts of these operations are two important goals towards sustainability. One approach to addressing these objectives is immunocontraception of fish which would increase the fish meat quality and yield in aquaculture production and prevent escaped farmed fish from undesirably altering wild fish population genetics through breeding. The research presented here was conducted with the aim of proof of concept for contraceptive vaccine use in Atlantic salmon. Nine vaccine formulations, including a negative control vaccine, were formulated at the National Wildlife Research Center in Fort Collins, Colorado and injected into farmed Atlantic salmon in Sunndalsøra, Norway. Production of antibodies against three immunogenic components in each vaccine formulation was evaluated over the course of the 12-week study. Weight and length of each fish were also tracked over time to determine whether growth was affected by vaccination. The study results indicate that Atlantic salmon will produce antibodies against BSA and KLH used as carrier proteins but that KLH is a stronger immunogen. Importantly, it was also determined that Atlantic salmon will produce antibodies against a small endogenous peptide (hapten) conjugated to the carrier protein, but to a lesser extent than the levels of antibody production against the carrier itself. Approximately 96% of samples from fish vaccinated against KLH, 76% of samples from fish vaccinated against BSA, and 36% of samples from fish vaccinated against the hapten were identified as positive. Response rates for all three antigens were highest 12 weeks post-vaccination. Significant differences in antibody levels were also ... Text Atlantic salmon Mountain Scholar (Digital Collections of Colorado and Wyoming) Norway Sunndalsøra ENVELOPE(8.563,8.563,62.675,62.675) |
institution |
Open Polar |
collection |
Mountain Scholar (Digital Collections of Colorado and Wyoming) |
op_collection_id |
ftmountainschol |
language |
English |
topic |
vaccine reproduction immunocontraception Atlantic salmon |
spellingShingle |
vaccine reproduction immunocontraception Atlantic salmon Orahood, Darcy Sonya Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon |
topic_facet |
vaccine reproduction immunocontraception Atlantic salmon |
description |
2013 Fall. Includes bibliographical references. Aquaculture is a rapidly growing industry that significantly contributes to the world food supply. Sustainable practices in aquaculture are of increasing importance as an increasing proportion of fish in the global market come from aquaculture instead of wild catch. Maximizing aquaculture yields and minimizing the ecological impacts of these operations are two important goals towards sustainability. One approach to addressing these objectives is immunocontraception of fish which would increase the fish meat quality and yield in aquaculture production and prevent escaped farmed fish from undesirably altering wild fish population genetics through breeding. The research presented here was conducted with the aim of proof of concept for contraceptive vaccine use in Atlantic salmon. Nine vaccine formulations, including a negative control vaccine, were formulated at the National Wildlife Research Center in Fort Collins, Colorado and injected into farmed Atlantic salmon in Sunndalsøra, Norway. Production of antibodies against three immunogenic components in each vaccine formulation was evaluated over the course of the 12-week study. Weight and length of each fish were also tracked over time to determine whether growth was affected by vaccination. The study results indicate that Atlantic salmon will produce antibodies against BSA and KLH used as carrier proteins but that KLH is a stronger immunogen. Importantly, it was also determined that Atlantic salmon will produce antibodies against a small endogenous peptide (hapten) conjugated to the carrier protein, but to a lesser extent than the levels of antibody production against the carrier itself. Approximately 96% of samples from fish vaccinated against KLH, 76% of samples from fish vaccinated against BSA, and 36% of samples from fish vaccinated against the hapten were identified as positive. Response rates for all three antigens were highest 12 weeks post-vaccination. Significant differences in antibody levels were also ... |
author2 |
Salman, M. D. Eckery, Douglas C. Miller, Lowell A. Myrick, Christopher A. Rhyan, Jack C. |
format |
Text |
author |
Orahood, Darcy Sonya |
author_facet |
Orahood, Darcy Sonya |
author_sort |
Orahood, Darcy Sonya |
title |
Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon |
title_short |
Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon |
title_full |
Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon |
title_fullStr |
Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon |
title_full_unstemmed |
Evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed Atlantic salmon |
title_sort |
evaluation of an adjuvanted hapten-protein vaccine approach to preventing sexual maturation of farmed atlantic salmon |
publisher |
Colorado State University. Libraries |
publishDate |
2007 |
url |
http://hdl.handle.net/10217/81048 |
long_lat |
ENVELOPE(8.563,8.563,62.675,62.675) |
geographic |
Norway Sunndalsøra |
geographic_facet |
Norway Sunndalsøra |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
2000-2019 - CSU Theses and Dissertations Orahood_colostate_0053N_12117.pdf ETDF2013500404CLIS http://hdl.handle.net/10217/81048 |
op_rights |
Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. |
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1768384501643214848 |