Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay
2012 Summer. Includes bibliographical references. A zoonotic disease that has killed millions over the course of at least three pandemics, plague remains a threat in regions where the etiologic agent, Yersinia pestis, persists in natural cycles involving small mammals and their fleas. Numerous flea...
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ftmountainschol:oai:mountainscholar.org:10217/67878 2023-06-11T04:16:16+02:00 Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay Graham, Christine B. Black, William C. Eisen, Rebecca J. Karkhoff-Schweizer, RoxAnn R. Huyvaert, Kathryn P. 2007-01-03T08:10:22Z born digital masters theses application/pdf http://hdl.handle.net/10217/67878 English eng eng Colorado State University. Libraries 2000-2019 - CSU Theses and Dissertations Graham_colostate_0053N_11208.pdf ETDF2012500158MIPA http://hdl.handle.net/10217/67878 Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. blood meal assay Uganda plague flea Text 2007 ftmountainschol 2023-04-29T17:46:26Z 2012 Summer. Includes bibliographical references. A zoonotic disease that has killed millions over the course of at least three pandemics, plague remains a threat in regions where the etiologic agent, Yersinia pestis, persists in natural cycles involving small mammals and their fleas. Numerous flea species have been implicated as Y. pestis vectors, and some provide a "bridge" from zoonotic hosts to humans, particularly during the epizootics that decimate susceptible small mammal populations. In order to serve as a bridging vector, a flea species must be able to transmit Y. pestis, it must feed on infectious zoonotic hosts, and it must feed on humans. Identifying bridging vector species in plague-endemic regions can aid in the development of vector-control activities aimed at reducing the incidence of human plague. The West Nile region is an established plague focus in northwest Uganda. Since 1999, more than 2400 suspect human plague cases have been reported from Vurra and Okoro counties. The most likely source of infection for humans in this region is the black rat, Rattus rattus, which commonly infests human habitations and is highly susceptible to Y. pestis infection. Other potential zoonotic hosts include other rodent and shrew species that predominate in the peridomestic environment and occasionally enter huts. Two rat flea species, Xenopsylla cheopis and X. brasiliensis, both among the most efficient flea vectors of Y. pestis, are very likely to serve as bridging vectors to humans in Vurra and Okoro counties. Recent investigations, however, have found that the cat flea, Ctenocephalides felis, comprises more than 88% of host-seeking (off-host) fleas captured in huts in this region. Though an inefficient vector, this species is capable of transmitting Y. pestis. Given its dominance in human habitations and its catholic feeding habits in other regions, we hypothesized that C. felis might serve as a secondary bridging vector in Vurra and Okoro counties. In order to address this hypothesis, we sought to ... Text Rattus rattus Mountain Scholar (Digital Collections of Colorado and Wyoming) |
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blood meal assay Uganda plague flea |
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blood meal assay Uganda plague flea Graham, Christine B. Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay |
topic_facet |
blood meal assay Uganda plague flea |
description |
2012 Summer. Includes bibliographical references. A zoonotic disease that has killed millions over the course of at least three pandemics, plague remains a threat in regions where the etiologic agent, Yersinia pestis, persists in natural cycles involving small mammals and their fleas. Numerous flea species have been implicated as Y. pestis vectors, and some provide a "bridge" from zoonotic hosts to humans, particularly during the epizootics that decimate susceptible small mammal populations. In order to serve as a bridging vector, a flea species must be able to transmit Y. pestis, it must feed on infectious zoonotic hosts, and it must feed on humans. Identifying bridging vector species in plague-endemic regions can aid in the development of vector-control activities aimed at reducing the incidence of human plague. The West Nile region is an established plague focus in northwest Uganda. Since 1999, more than 2400 suspect human plague cases have been reported from Vurra and Okoro counties. The most likely source of infection for humans in this region is the black rat, Rattus rattus, which commonly infests human habitations and is highly susceptible to Y. pestis infection. Other potential zoonotic hosts include other rodent and shrew species that predominate in the peridomestic environment and occasionally enter huts. Two rat flea species, Xenopsylla cheopis and X. brasiliensis, both among the most efficient flea vectors of Y. pestis, are very likely to serve as bridging vectors to humans in Vurra and Okoro counties. Recent investigations, however, have found that the cat flea, Ctenocephalides felis, comprises more than 88% of host-seeking (off-host) fleas captured in huts in this region. Though an inefficient vector, this species is capable of transmitting Y. pestis. Given its dominance in human habitations and its catholic feeding habits in other regions, we hypothesized that C. felis might serve as a secondary bridging vector in Vurra and Okoro counties. In order to address this hypothesis, we sought to ... |
author2 |
Black, William C. Eisen, Rebecca J. Karkhoff-Schweizer, RoxAnn R. Huyvaert, Kathryn P. |
format |
Text |
author |
Graham, Christine B. |
author_facet |
Graham, Christine B. |
author_sort |
Graham, Christine B. |
title |
Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay |
title_short |
Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay |
title_full |
Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay |
title_fullStr |
Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay |
title_full_unstemmed |
Identifying blood meals in cat fleas (Ctenocephalides felis) from a plague-endemic region of Uganda using a SYBR Green real-time polymerase chain reaction-based assay |
title_sort |
identifying blood meals in cat fleas (ctenocephalides felis) from a plague-endemic region of uganda using a sybr green real-time polymerase chain reaction-based assay |
publisher |
Colorado State University. Libraries |
publishDate |
2007 |
url |
http://hdl.handle.net/10217/67878 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
2000-2019 - CSU Theses and Dissertations Graham_colostate_0053N_11208.pdf ETDF2012500158MIPA http://hdl.handle.net/10217/67878 |
op_rights |
Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. |
_version_ |
1768373857567113216 |