The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems
Microorganisms interact with their surroundings and each other. However, these interactions are complex and difficult to understand. This research presents the utilization of simplified environmental microbial systems from the extremes of life to gain insight into the roles of microbes in diverse pr...
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Montana State University - Bozeman, College of Letters & Science
2015
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ftmontanastateu:oai:scholarworks.montana.edu:1/10146 2023-05-15T13:44:28+02:00 The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems Tigges, Michelle Marie Chairperson, Graduate Committee: Brian Bothner Michelle Tigges and Heidi Smith were main authors, and Juliana D'Andrill, Al Parker, Brian Bothner and Christine Foreman were co-authors of the article, 'Lability of environmental dissolved organic matter drives dynamic microbial processing' submitted to the journal 'Proceedings of the National Academy of Sciences' which is contained within this thesis. 2015 application/pdf https://scholarworks.montana.edu/xmlui/handle/1/10146 en eng Montana State University - Bozeman, College of Letters & Science https://scholarworks.montana.edu/xmlui/handle/1/10146 Copyright 2015 by Michelle Marie Tigges Microorganisms Mass spectrometry Organic compounds Dissertation 2015 ftmontanastateu 2022-08-13T22:40:34Z Microorganisms interact with their surroundings and each other. However, these interactions are complex and difficult to understand. This research presents the utilization of simplified environmental microbial systems from the extremes of life to gain insight into the roles of microbes in diverse processes including biogeochemical cycling and viral infection. The complex mixture of organic compounds within aquatic systems, known as dissolved organic matter (DOM), is an integral component of the global carbon cycle. It is a carbon source for microbial activity and impacts biogeochemical and ecological processes. However, little is known about the release and bioconversion of these compounds. This thesis presents a liquid chromatography coupled mass spectrometry (LCMS) based exometabolomics approach to chemically characterize the interaction between DOM and the representative microbial species that transform it. This work illustrates for the first time the ability to measure the relative abundance of molecular constituents of DOM during heterotrophic bacterial processing. Processing was shown to be dynamic over time, even with only single organism interactions. A LCMS based proxy was established to predict the lability of DOM carbon sources, and the labile nature of the source was shown to be a significant factor in DOM processing by single organisms. Further, the temporal interaction of two ecologically relevant beta-Proteobacteria with DOM from the Cotton Glacier, Antarctica highlight the importance of understanding the diversity of single organism DOM interactions to interpret community level bacterial interactions. LCMS-based 'omics techniques can also be utilized to characterize the changes in protein expression associated with viral infection of hyperthermophilic archaea. Viral-host interactions in Sulfolobus archaeal systems are poorly understood, and exhibit a diversity of regulation patterns. LCMS-based shotgun proteomics was utilized to characterize the temporal response of Sulfolobus islandicus to ... Doctoral or Postdoctoral Thesis Antarc* Antarctica Montana State University (MSU): ScholarWorks Cotton Glacier ENVELOPE(161.667,161.667,-77.117,-77.117) |
institution |
Open Polar |
collection |
Montana State University (MSU): ScholarWorks |
op_collection_id |
ftmontanastateu |
language |
English |
topic |
Microorganisms Mass spectrometry Organic compounds |
spellingShingle |
Microorganisms Mass spectrometry Organic compounds Tigges, Michelle Marie The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems |
topic_facet |
Microorganisms Mass spectrometry Organic compounds |
description |
Microorganisms interact with their surroundings and each other. However, these interactions are complex and difficult to understand. This research presents the utilization of simplified environmental microbial systems from the extremes of life to gain insight into the roles of microbes in diverse processes including biogeochemical cycling and viral infection. The complex mixture of organic compounds within aquatic systems, known as dissolved organic matter (DOM), is an integral component of the global carbon cycle. It is a carbon source for microbial activity and impacts biogeochemical and ecological processes. However, little is known about the release and bioconversion of these compounds. This thesis presents a liquid chromatography coupled mass spectrometry (LCMS) based exometabolomics approach to chemically characterize the interaction between DOM and the representative microbial species that transform it. This work illustrates for the first time the ability to measure the relative abundance of molecular constituents of DOM during heterotrophic bacterial processing. Processing was shown to be dynamic over time, even with only single organism interactions. A LCMS based proxy was established to predict the lability of DOM carbon sources, and the labile nature of the source was shown to be a significant factor in DOM processing by single organisms. Further, the temporal interaction of two ecologically relevant beta-Proteobacteria with DOM from the Cotton Glacier, Antarctica highlight the importance of understanding the diversity of single organism DOM interactions to interpret community level bacterial interactions. LCMS-based 'omics techniques can also be utilized to characterize the changes in protein expression associated with viral infection of hyperthermophilic archaea. Viral-host interactions in Sulfolobus archaeal systems are poorly understood, and exhibit a diversity of regulation patterns. LCMS-based shotgun proteomics was utilized to characterize the temporal response of Sulfolobus islandicus to ... |
author2 |
Chairperson, Graduate Committee: Brian Bothner Michelle Tigges and Heidi Smith were main authors, and Juliana D'Andrill, Al Parker, Brian Bothner and Christine Foreman were co-authors of the article, 'Lability of environmental dissolved organic matter drives dynamic microbial processing' submitted to the journal 'Proceedings of the National Academy of Sciences' which is contained within this thesis. |
format |
Doctoral or Postdoctoral Thesis |
author |
Tigges, Michelle Marie |
author_facet |
Tigges, Michelle Marie |
author_sort |
Tigges, Michelle Marie |
title |
The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems |
title_short |
The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems |
title_full |
The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems |
title_fullStr |
The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems |
title_full_unstemmed |
The application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems |
title_sort |
application of mass spectrometry-based 'omics technologies to investigate environmental interactions of microbial systems |
publisher |
Montana State University - Bozeman, College of Letters & Science |
publishDate |
2015 |
url |
https://scholarworks.montana.edu/xmlui/handle/1/10146 |
long_lat |
ENVELOPE(161.667,161.667,-77.117,-77.117) |
geographic |
Cotton Glacier |
geographic_facet |
Cotton Glacier |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://scholarworks.montana.edu/xmlui/handle/1/10146 |
op_rights |
Copyright 2015 by Michelle Marie Tigges |
_version_ |
1766202018754461696 |