Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases
BACKGROUND: Crude palm oil (CPO) is rich with phytonutrients such as carotenoids and tocols which possesses many health benefits. The aim of this research was to develop a methanol-free process to produce palm phytonutrients via enzymatic hydrolysis. In this work, triacylglycerol was hydrolyzed into...
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Online Access: | https://research.monash.edu/en/publications/9636f443-1ab4-4cc9-90cc-68f241088cfc https://doi.org/10.1002/jsfa.12053 https://researchmgt.monash.edu/ws/files/415774175/383501938_oa.pdf http://www.scopus.com/inward/record.url?scp=85132384993&partnerID=8YFLogxK |
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ftmonashunicris:oai:monash.edu:publications/9636f443-1ab4-4cc9-90cc-68f241088cfc 2023-05-15T14:15:27+02:00 Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases Adiiba, Siti Hanifah Chan, Eng-Seng Lee, Yee Ying Amelia, Chang, Mun Yuen Song, Cher Pin 2022-12 application/pdf https://research.monash.edu/en/publications/9636f443-1ab4-4cc9-90cc-68f241088cfc https://doi.org/10.1002/jsfa.12053 https://researchmgt.monash.edu/ws/files/415774175/383501938_oa.pdf http://www.scopus.com/inward/record.url?scp=85132384993&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Adiiba , S H , Chan , E-S , Lee , Y Y , Amelia , Chang , M Y & Song , C P 2022 , ' Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases ' , Journal of the Science of Food and Agriculture , vol. 102 , no. 15 , pp. 6921-6929 . https://doi.org/10.1002/jsfa.12053 carotene crude palm oil enzymatic hydrolysis lipases phytonutrients tocols article 2022 ftmonashunicris https://doi.org/10.1002/jsfa.12053 2023-02-05T06:41:21Z BACKGROUND: Crude palm oil (CPO) is rich with phytonutrients such as carotenoids and tocols which possesses many health benefits. The aim of this research was to develop a methanol-free process to produce palm phytonutrients via enzymatic hydrolysis. In this work, triacylglycerol was hydrolyzed into free fatty acids (FFAs) using three different types of liquid lipases derived from Aspergillus oryzae (ET 2.0), Aspergillus niger (Habio) and Candida antartica (CALB). RESULTS: ET 2.0 was found to be the best enzyme for hydrolysis. Under the optimum condition, the FFA content achievable was 790 g kg −1 after 24 h of reaction with 1:1 water-to-oil mass ratio at 50 °C and stirring speed of 9 × g. Furthermore, with the addition of 2 g kg −1 ascorbic acid, it was found that 98% of carotenoids and 96% of tocols could be retained after hydrolysis. CONCLUSION: This work shows that enzymatic hydrolysis, which is inherently safer, cleaner and sustainable is feasible to replace the conventional methanolysis for the production of palm phytonutrients. Article in Journal/Newspaper antartic* Monash University Research Portal Journal of the Science of Food and Agriculture 102 15 6921 6929 |
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Open Polar |
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Monash University Research Portal |
op_collection_id |
ftmonashunicris |
language |
English |
topic |
carotene crude palm oil enzymatic hydrolysis lipases phytonutrients tocols |
spellingShingle |
carotene crude palm oil enzymatic hydrolysis lipases phytonutrients tocols Adiiba, Siti Hanifah Chan, Eng-Seng Lee, Yee Ying Amelia, Chang, Mun Yuen Song, Cher Pin Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases |
topic_facet |
carotene crude palm oil enzymatic hydrolysis lipases phytonutrients tocols |
description |
BACKGROUND: Crude palm oil (CPO) is rich with phytonutrients such as carotenoids and tocols which possesses many health benefits. The aim of this research was to develop a methanol-free process to produce palm phytonutrients via enzymatic hydrolysis. In this work, triacylglycerol was hydrolyzed into free fatty acids (FFAs) using three different types of liquid lipases derived from Aspergillus oryzae (ET 2.0), Aspergillus niger (Habio) and Candida antartica (CALB). RESULTS: ET 2.0 was found to be the best enzyme for hydrolysis. Under the optimum condition, the FFA content achievable was 790 g kg −1 after 24 h of reaction with 1:1 water-to-oil mass ratio at 50 °C and stirring speed of 9 × g. Furthermore, with the addition of 2 g kg −1 ascorbic acid, it was found that 98% of carotenoids and 96% of tocols could be retained after hydrolysis. CONCLUSION: This work shows that enzymatic hydrolysis, which is inherently safer, cleaner and sustainable is feasible to replace the conventional methanolysis for the production of palm phytonutrients. |
format |
Article in Journal/Newspaper |
author |
Adiiba, Siti Hanifah Chan, Eng-Seng Lee, Yee Ying Amelia, Chang, Mun Yuen Song, Cher Pin |
author_facet |
Adiiba, Siti Hanifah Chan, Eng-Seng Lee, Yee Ying Amelia, Chang, Mun Yuen Song, Cher Pin |
author_sort |
Adiiba, Siti Hanifah |
title |
Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases |
title_short |
Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases |
title_full |
Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases |
title_fullStr |
Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases |
title_full_unstemmed |
Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases |
title_sort |
towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases |
publishDate |
2022 |
url |
https://research.monash.edu/en/publications/9636f443-1ab4-4cc9-90cc-68f241088cfc https://doi.org/10.1002/jsfa.12053 https://researchmgt.monash.edu/ws/files/415774175/383501938_oa.pdf http://www.scopus.com/inward/record.url?scp=85132384993&partnerID=8YFLogxK |
genre |
antartic* |
genre_facet |
antartic* |
op_source |
Adiiba , S H , Chan , E-S , Lee , Y Y , Amelia , Chang , M Y & Song , C P 2022 , ' Towards an alcohol-free process for the production of palm phytonutrients via enzymatic hydrolysis of crude palm oil using liquid lipases ' , Journal of the Science of Food and Agriculture , vol. 102 , no. 15 , pp. 6921-6929 . https://doi.org/10.1002/jsfa.12053 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/jsfa.12053 |
container_title |
Journal of the Science of Food and Agriculture |
container_volume |
102 |
container_issue |
15 |
container_start_page |
6921 |
op_container_end_page |
6929 |
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1766287831507927040 |