Ocean acidification modulates expression of genes and physiological performance of a marine diatom
Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic...
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Online Access: | https://research.monash.edu/en/publications/7c330368-34a5-407a-8434-4a4470c5c7b0 https://doi.org/10.1371/journal.pone.0170970 https://researchmgt.monash.edu/ws/files/26156132/25253515_oa.pdf http://www.scopus.com/inward/record.url?scp=85012190882&partnerID=8YFLogxK |
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ftmonashunicris:oai:monash.edu:publications/7c330368-34a5-407a-8434-4a4470c5c7b0 2024-06-23T07:55:50+00:00 Ocean acidification modulates expression of genes and physiological performance of a marine diatom Li, Yahe Zhuang, Shufang Wu, Yaping Ren, Honglin Chen, Fangyi Lin, Xin Wang, Kejian Beardall, John Gao, Kunshan 2017-02-01 application/pdf https://research.monash.edu/en/publications/7c330368-34a5-407a-8434-4a4470c5c7b0 https://doi.org/10.1371/journal.pone.0170970 https://researchmgt.monash.edu/ws/files/26156132/25253515_oa.pdf http://www.scopus.com/inward/record.url?scp=85012190882&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Li , Y , Zhuang , S , Wu , Y , Ren , H , Chen , F , Lin , X , Wang , K , Beardall , J & Gao , K 2017 , ' Ocean acidification modulates expression of genes and physiological performance of a marine diatom ' , PLoS ONE , vol. 12 , no. 2 , e0170970 . https://doi.org/10.1371/journal.pone.0170970 article 2017 ftmonashunicris https://doi.org/10.1371/journal.pone.0170970 2024-06-06T00:05:37Z Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic light harvesting as well as those encoding Rubisco, carbonic anhydrase, NADH dehydrogenase and nitrite reductase, are modulated by OA (1000 μatm, pH nbs 7.83). Growth and photosynthetic carbon fixation were enhanced by elevated CO 2 . OA treatment decreased the expression of β-carbonic anhydrase (β-ca), which functions in balancing intracellular carbonate chemistry and the CO 2 concentrating mechanism (CCM). The expression of the genes encoding fucoxanthin chlorophyll a/c protein (lhcf type (fcp)), mitochondrial ATP synthase (mtATP), ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene (rbcl) and NADH dehydrogenase subunit 2(ndh2), were down-regulated during the first four days (< 8 generations) after the cells were transferred from LC (cells grown under ambient air condition; 390 μatm; pH nbs 8.19) to OA conditions, with no significant difference between LC and HC treatments with the time elapsed. The expression of nitrite reductase (nir) was up-regulated by the OA treatment. Additionally, the genes for these proteins (NiR, FCP, mtATP synthase, β-CA) showed diel expression patterns. It appeared that the enhanced photosynthetic and growth rates under OA could be attributed to stimulated nitrogen assimilation, increased CO 2 availability or saved energy from downregulation of the CCM and consequently lowered cost of protein synthesis versus that of non-nitrogenous cell components. Article in Journal/Newspaper Ocean acidification Monash University Research Portal PLOS ONE 12 2 e0170970 |
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Open Polar |
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Monash University Research Portal |
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ftmonashunicris |
language |
English |
description |
Ocean Acidification (OA) is known to affect various aspects of physiological performances of diatoms, but little is known about the underlining molecular mechanisms involved. Here, we show that in the model diatom Phaeodactylum tricornutum, the expression of key genes associated with photosynthetic light harvesting as well as those encoding Rubisco, carbonic anhydrase, NADH dehydrogenase and nitrite reductase, are modulated by OA (1000 μatm, pH nbs 7.83). Growth and photosynthetic carbon fixation were enhanced by elevated CO 2 . OA treatment decreased the expression of β-carbonic anhydrase (β-ca), which functions in balancing intracellular carbonate chemistry and the CO 2 concentrating mechanism (CCM). The expression of the genes encoding fucoxanthin chlorophyll a/c protein (lhcf type (fcp)), mitochondrial ATP synthase (mtATP), ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene (rbcl) and NADH dehydrogenase subunit 2(ndh2), were down-regulated during the first four days (< 8 generations) after the cells were transferred from LC (cells grown under ambient air condition; 390 μatm; pH nbs 8.19) to OA conditions, with no significant difference between LC and HC treatments with the time elapsed. The expression of nitrite reductase (nir) was up-regulated by the OA treatment. Additionally, the genes for these proteins (NiR, FCP, mtATP synthase, β-CA) showed diel expression patterns. It appeared that the enhanced photosynthetic and growth rates under OA could be attributed to stimulated nitrogen assimilation, increased CO 2 availability or saved energy from downregulation of the CCM and consequently lowered cost of protein synthesis versus that of non-nitrogenous cell components. |
format |
Article in Journal/Newspaper |
author |
Li, Yahe Zhuang, Shufang Wu, Yaping Ren, Honglin Chen, Fangyi Lin, Xin Wang, Kejian Beardall, John Gao, Kunshan |
spellingShingle |
Li, Yahe Zhuang, Shufang Wu, Yaping Ren, Honglin Chen, Fangyi Lin, Xin Wang, Kejian Beardall, John Gao, Kunshan Ocean acidification modulates expression of genes and physiological performance of a marine diatom |
author_facet |
Li, Yahe Zhuang, Shufang Wu, Yaping Ren, Honglin Chen, Fangyi Lin, Xin Wang, Kejian Beardall, John Gao, Kunshan |
author_sort |
Li, Yahe |
title |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom |
title_short |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom |
title_full |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom |
title_fullStr |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom |
title_full_unstemmed |
Ocean acidification modulates expression of genes and physiological performance of a marine diatom |
title_sort |
ocean acidification modulates expression of genes and physiological performance of a marine diatom |
publishDate |
2017 |
url |
https://research.monash.edu/en/publications/7c330368-34a5-407a-8434-4a4470c5c7b0 https://doi.org/10.1371/journal.pone.0170970 https://researchmgt.monash.edu/ws/files/26156132/25253515_oa.pdf http://www.scopus.com/inward/record.url?scp=85012190882&partnerID=8YFLogxK |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Li , Y , Zhuang , S , Wu , Y , Ren , H , Chen , F , Lin , X , Wang , K , Beardall , J & Gao , K 2017 , ' Ocean acidification modulates expression of genes and physiological performance of a marine diatom ' , PLoS ONE , vol. 12 , no. 2 , e0170970 . https://doi.org/10.1371/journal.pone.0170970 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0170970 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
2 |
container_start_page |
e0170970 |
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