Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms
There is limited information on the cross-neutralisation of neurotoxic venoms with antivenoms. Cross-neutralisation of the in vitro neurotoxicity of four Asian and four Australian snake venoms, four post-synaptic neurotoxins (α-bungarotoxin, α-elapitoxin-Nk2a, α-elapitoxin-Ppr1 and α-scutoxin; 100 n...
Published in: | Toxins |
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2016
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Online Access: | https://research.monash.edu/en/publications/172bdf30-2823-4842-b6cb-93cfb1fedb81 https://doi.org/10.3390/toxins8100302 https://researchmgt.monash.edu/ws/files/53594393/15343134_oa.pdf http://www.scopus.com/inward/record.url?scp=84992029443&partnerID=8YFLogxK |
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ftmonashunicris:oai:monash.edu:publications/172bdf30-2823-4842-b6cb-93cfb1fedb81 2024-09-09T19:05:09+00:00 Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms Silva, Anjana Hodgson, Wayne C Isbister, Geoffrey K 2016-10-01 application/pdf https://research.monash.edu/en/publications/172bdf30-2823-4842-b6cb-93cfb1fedb81 https://doi.org/10.3390/toxins8100302 https://researchmgt.monash.edu/ws/files/53594393/15343134_oa.pdf http://www.scopus.com/inward/record.url?scp=84992029443&partnerID=8YFLogxK eng eng https://research.monash.edu/en/publications/172bdf30-2823-4842-b6cb-93cfb1fedb81 info:eu-repo/semantics/openAccess Silva , A , Hodgson , W C & Isbister , G K 2016 , ' Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms ' , Toxins , vol. 8 , no. 10 , 302 . https://doi.org/10.3390/toxins8100302 Antivenom Cross-neutralisation Neurotoxicity Snake Venom article 2016 ftmonashunicris https://doi.org/10.3390/toxins8100302 2024-08-21T23:55:40Z There is limited information on the cross-neutralisation of neurotoxic venoms with antivenoms. Cross-neutralisation of the in vitro neurotoxicity of four Asian and four Australian snake venoms, four post-synaptic neurotoxins (α-bungarotoxin, α-elapitoxin-Nk2a, α-elapitoxin-Ppr1 and α-scutoxin; 100 nM) and one pre-synaptic neurotoxin (taipoxin; 100 nM) was studied with five antivenoms: Thai cobra antivenom (TCAV), death adder antivenom (DAAV), Thai neuro polyvalent antivenom (TNPAV), Indian Polyvalent antivenom (IPAV) and Australian polyvalent antivenom (APAV). The chick biventer cervicis nerve-muscle preparation was used for this study. Antivenom was added to the organ bath 20 min prior to venom. Pre- and post-synaptic neurotoxicity of Bungarus caeruleus and Bungarus fasciatus venoms was neutralised by all antivenoms except TCAV, which did not neutralise pre-synaptic activity. Post-synaptic neurotoxicity of Ophiophagus hannah was neutralised by all antivenoms, and Naja kaouthia by all antivenoms except IPAV. Pre- and post-synaptic neurotoxicity of Notechis scutatus was neutralised by all antivenoms, except TCAV, which only partially neutralised pre-synaptic activity. Pre- and post-synaptic neurotoxicity of Oxyuranus scutellatus was neutralised by TNPAV and APAV, but TCAV and IPAV only neutralised post-synaptic neurotoxicity. Post-synaptic neurotoxicity of Acanthophis antarcticus was neutralised by all antivenoms except IPAV. Pseudonaja textillis post-synaptic neurotoxicity was only neutralised by APAV. The α-neurotoxins were neutralised by TNPAV and APAV, and taipoxin by all antivenoms except IPAV. Antivenoms raised against venoms with post-synaptic neurotoxic activity (TCAV) cross-neutralised the post-synaptic activity of multiple snake venoms. Antivenoms raised against pre- and post-synaptic neurotoxic venoms (TNPAV, IPAV, APAV) cross-neutralised both activities of Asian and Australian venoms. While acknowledging the limitations of adding antivenom prior to venom in an in vitro preparation, ... Article in Journal/Newspaper Antarc* antarcticus Monash University Research Portal Hannah ENVELOPE(-60.613,-60.613,-62.654,-62.654) Indian Toxins 8 10 302 |
institution |
Open Polar |
collection |
Monash University Research Portal |
op_collection_id |
ftmonashunicris |
language |
English |
topic |
Antivenom Cross-neutralisation Neurotoxicity Snake Venom |
spellingShingle |
Antivenom Cross-neutralisation Neurotoxicity Snake Venom Silva, Anjana Hodgson, Wayne C Isbister, Geoffrey K Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms |
topic_facet |
Antivenom Cross-neutralisation Neurotoxicity Snake Venom |
description |
There is limited information on the cross-neutralisation of neurotoxic venoms with antivenoms. Cross-neutralisation of the in vitro neurotoxicity of four Asian and four Australian snake venoms, four post-synaptic neurotoxins (α-bungarotoxin, α-elapitoxin-Nk2a, α-elapitoxin-Ppr1 and α-scutoxin; 100 nM) and one pre-synaptic neurotoxin (taipoxin; 100 nM) was studied with five antivenoms: Thai cobra antivenom (TCAV), death adder antivenom (DAAV), Thai neuro polyvalent antivenom (TNPAV), Indian Polyvalent antivenom (IPAV) and Australian polyvalent antivenom (APAV). The chick biventer cervicis nerve-muscle preparation was used for this study. Antivenom was added to the organ bath 20 min prior to venom. Pre- and post-synaptic neurotoxicity of Bungarus caeruleus and Bungarus fasciatus venoms was neutralised by all antivenoms except TCAV, which did not neutralise pre-synaptic activity. Post-synaptic neurotoxicity of Ophiophagus hannah was neutralised by all antivenoms, and Naja kaouthia by all antivenoms except IPAV. Pre- and post-synaptic neurotoxicity of Notechis scutatus was neutralised by all antivenoms, except TCAV, which only partially neutralised pre-synaptic activity. Pre- and post-synaptic neurotoxicity of Oxyuranus scutellatus was neutralised by TNPAV and APAV, but TCAV and IPAV only neutralised post-synaptic neurotoxicity. Post-synaptic neurotoxicity of Acanthophis antarcticus was neutralised by all antivenoms except IPAV. Pseudonaja textillis post-synaptic neurotoxicity was only neutralised by APAV. The α-neurotoxins were neutralised by TNPAV and APAV, and taipoxin by all antivenoms except IPAV. Antivenoms raised against venoms with post-synaptic neurotoxic activity (TCAV) cross-neutralised the post-synaptic activity of multiple snake venoms. Antivenoms raised against pre- and post-synaptic neurotoxic venoms (TNPAV, IPAV, APAV) cross-neutralised both activities of Asian and Australian venoms. While acknowledging the limitations of adding antivenom prior to venom in an in vitro preparation, ... |
format |
Article in Journal/Newspaper |
author |
Silva, Anjana Hodgson, Wayne C Isbister, Geoffrey K |
author_facet |
Silva, Anjana Hodgson, Wayne C Isbister, Geoffrey K |
author_sort |
Silva, Anjana |
title |
Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms |
title_short |
Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms |
title_full |
Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms |
title_fullStr |
Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms |
title_full_unstemmed |
Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms |
title_sort |
cross-neutralisation of in vitro neurotoxicity of asian and australian snake neurotoxins and venoms by different antivenoms |
publishDate |
2016 |
url |
https://research.monash.edu/en/publications/172bdf30-2823-4842-b6cb-93cfb1fedb81 https://doi.org/10.3390/toxins8100302 https://researchmgt.monash.edu/ws/files/53594393/15343134_oa.pdf http://www.scopus.com/inward/record.url?scp=84992029443&partnerID=8YFLogxK |
long_lat |
ENVELOPE(-60.613,-60.613,-62.654,-62.654) |
geographic |
Hannah Indian |
geographic_facet |
Hannah Indian |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_source |
Silva , A , Hodgson , W C & Isbister , G K 2016 , ' Cross-neutralisation of in vitro neurotoxicity of asian and Australian snake neurotoxins and venoms by different antivenoms ' , Toxins , vol. 8 , no. 10 , 302 . https://doi.org/10.3390/toxins8100302 |
op_relation |
https://research.monash.edu/en/publications/172bdf30-2823-4842-b6cb-93cfb1fedb81 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/toxins8100302 |
container_title |
Toxins |
container_volume |
8 |
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10 |
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302 |
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1809819115547787264 |